In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf
Absolute methanol extract leaf of Costus afer was comprehensively investigated for free radical scavenging activities and antioxidant activities using battery of testing: 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, hydroxyl radical scavenging, hydrogen peroxide radical scavenging, 2, 2′-azino-...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2018-12-01
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| Series: | Food Science and Human Wellness |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453017301945 |
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| author | T.G. Atere O.A. Akinloye R.N. Ugbaja D.A. Ojo G. Dealtry |
| author_facet | T.G. Atere O.A. Akinloye R.N. Ugbaja D.A. Ojo G. Dealtry |
| author_sort | T.G. Atere |
| collection | DOAJ |
| description | Absolute methanol extract leaf of Costus afer was comprehensively investigated for free radical scavenging activities and antioxidant activities using battery of testing: 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, hydroxyl radical scavenging, hydrogen peroxide radical scavenging, 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging, nitric oxide inhibition, anti-lipid peroxidation, ferrous chelating potential, reducing power potential, total antioxidant capacity, cupric reducing antioxidant capacity CUPRAC and ferric reducing antioxidant power (FRAP). The content of phenol, flavonoid, cupric, anthocyanin were also investigated. The results suggest that the antioxidant potentials of the extract may be responsible for its medicinal potentials. Cell viability assay revealed that up to12 μg/ml of the extract is safe for Chang liver cell and no sign of toxicity was observed after the extract treatment of 2000 mg/kg in albino rats. Keywords: Costus afer, Antioxidant, Toxicity |
| format | Article |
| id | doaj-art-bcb054876419416397b85c7c82eb0456 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-bcb054876419416397b85c7c82eb04562025-02-02T23:24:55ZengTsinghua University PressFood Science and Human Wellness2213-45302018-12-0174266272In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leafT.G. Atere0O.A. Akinloye1R.N. Ugbaja2D.A. Ojo3G. Dealtry4Department of Medical Biochemistry, College of Health Sciences, Osun State University, Osogbo, Nigeria; Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria; Corresponding author at: Department of Medical Biochemistry, College of Health Sciences, Osun State University, Osogbo, Nigeria.Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, NigeriaDepartment of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, NigeriaDepartment of Microbiology, College of Biosciences, Federal University of Agriculture, Abeokuta, NigeriaDepartment of Biochemistry and Microbiology, Nelson Mandela Metropolitan University, Port Elizabeth, 6031, South AfricaAbsolute methanol extract leaf of Costus afer was comprehensively investigated for free radical scavenging activities and antioxidant activities using battery of testing: 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, hydroxyl radical scavenging, hydrogen peroxide radical scavenging, 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging, nitric oxide inhibition, anti-lipid peroxidation, ferrous chelating potential, reducing power potential, total antioxidant capacity, cupric reducing antioxidant capacity CUPRAC and ferric reducing antioxidant power (FRAP). The content of phenol, flavonoid, cupric, anthocyanin were also investigated. The results suggest that the antioxidant potentials of the extract may be responsible for its medicinal potentials. Cell viability assay revealed that up to12 μg/ml of the extract is safe for Chang liver cell and no sign of toxicity was observed after the extract treatment of 2000 mg/kg in albino rats. Keywords: Costus afer, Antioxidant, Toxicityhttp://www.sciencedirect.com/science/article/pii/S2213453017301945 |
| spellingShingle | T.G. Atere O.A. Akinloye R.N. Ugbaja D.A. Ojo G. Dealtry In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf Food Science and Human Wellness |
| title | In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf |
| title_full | In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf |
| title_fullStr | In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf |
| title_full_unstemmed | In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf |
| title_short | In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf |
| title_sort | in vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of costus afer leaf |
| url | http://www.sciencedirect.com/science/article/pii/S2213453017301945 |
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