Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High‐Cryotolerant Rams or Extender
ABSTRACT Ram semen cryotolerance problem continues despite intensive research. This study aimed to evaluate the effect of replacing total seminal plasma (SP) with that of high‐cryotolerant rams or extender on semen quality parameters in low‐cryotolerant rams. Rams previously determined as low (n = 6...
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| Format: | Article |
| Language: | English |
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Wiley
2025-05-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70381 |
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| author | Cemal Dayanıklı Bülent Bülbül Şükrü Doğan Ebru Şengül Mesut Kırbaş Yavuz Kal Mehmet Bozkurt Ataman |
| author_facet | Cemal Dayanıklı Bülent Bülbül Şükrü Doğan Ebru Şengül Mesut Kırbaş Yavuz Kal Mehmet Bozkurt Ataman |
| author_sort | Cemal Dayanıklı |
| collection | DOAJ |
| description | ABSTRACT Ram semen cryotolerance problem continues despite intensive research. This study aimed to evaluate the effect of replacing total seminal plasma (SP) with that of high‐cryotolerant rams or extender on semen quality parameters in low‐cryotolerant rams. Rams previously determined as low (n = 6) and high‐cryotolerant (n = 5) were used in the breeding season. High‐cryotolerant rams were only included with their SP in the SP+ group. Each normospermic fresh ejaculate from low‐cryotolerant rams was split into three aliquots to form the groups. The control aliquot was diluted using a two‐step process. Centrifugation removed SP from the second aliquot (SP− group), and the sperm pellet was rediluted with a Tris‐based extender using the same amount of SP removed. The third aliquot's SP was removed as in the SP− group, but the remaining sperm pellet was rediluted with fresh SP harvested from high‐cryotolerant rams using the same amounts of removed SP in the SP+ group. Semen was rediluted with a Tris‐based extender in the SP− and SP+ groups using a two‐step process as in the control group. Sperm quality was similar between the groups after dilution and equilibration. However, the post‐thaw semen quality was higher in the SP− (one parameter) and SP+ (four parameters) groups than in the control group (p < 0.05). Additionally, these parameters were similar in the SP− and SP+ groups. This study showed that, although an adequate extender had positive effects, replacing the whole SP with that of high‐cryotolerant rams may better solve the low cryotolerance problem in ram semen. |
| format | Article |
| id | doaj-art-bc9e6f7449d74071aa195320f136baf9 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-bc9e6f7449d74071aa195320f136baf92025-08-20T03:47:58ZengWileyVeterinary Medicine and Science2053-10952025-05-01113n/an/a10.1002/vms3.70381Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High‐Cryotolerant Rams or ExtenderCemal Dayanıklı0Bülent Bülbül1Şükrü Doğan2Ebru Şengül3Mesut Kırbaş4Yavuz Kal5Mehmet Bozkurt Ataman6Department of Breeding Techniques Sheep Breeding Research Institute Balıkesir TürkiyeDepartment of Reproduction and Artificial Insemination Faculty of Veterinary Medicine Dokuz Eylül University İzmir TürkiyeDepartment of Animal Breeding Bahri Dağdaş International Agricultural Research Institute Konya TürkiyeDepartment of Breeding Techniques Sheep Breeding Research Institute Balıkesir TürkiyeDepartment of Animal Breeding Bahri Dağdaş International Agricultural Research Institute Konya TürkiyeDepartment of Animal Breeding Bahri Dağdaş International Agricultural Research Institute Konya TürkiyeDepartment of Reproduction and Artificial Insemination Faculty of Veterinary Medicine Selçuk University Konya TürkiyeABSTRACT Ram semen cryotolerance problem continues despite intensive research. This study aimed to evaluate the effect of replacing total seminal plasma (SP) with that of high‐cryotolerant rams or extender on semen quality parameters in low‐cryotolerant rams. Rams previously determined as low (n = 6) and high‐cryotolerant (n = 5) were used in the breeding season. High‐cryotolerant rams were only included with their SP in the SP+ group. Each normospermic fresh ejaculate from low‐cryotolerant rams was split into three aliquots to form the groups. The control aliquot was diluted using a two‐step process. Centrifugation removed SP from the second aliquot (SP− group), and the sperm pellet was rediluted with a Tris‐based extender using the same amount of SP removed. The third aliquot's SP was removed as in the SP− group, but the remaining sperm pellet was rediluted with fresh SP harvested from high‐cryotolerant rams using the same amounts of removed SP in the SP+ group. Semen was rediluted with a Tris‐based extender in the SP− and SP+ groups using a two‐step process as in the control group. Sperm quality was similar between the groups after dilution and equilibration. However, the post‐thaw semen quality was higher in the SP− (one parameter) and SP+ (four parameters) groups than in the control group (p < 0.05). Additionally, these parameters were similar in the SP− and SP+ groups. This study showed that, although an adequate extender had positive effects, replacing the whole SP with that of high‐cryotolerant rams may better solve the low cryotolerance problem in ram semen.https://doi.org/10.1002/vms3.70381cryotoleranceextenderramsemenseminal plasma |
| spellingShingle | Cemal Dayanıklı Bülent Bülbül Şükrü Doğan Ebru Şengül Mesut Kırbaş Yavuz Kal Mehmet Bozkurt Ataman Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High‐Cryotolerant Rams or Extender Veterinary Medicine and Science cryotolerance extender ram semen seminal plasma |
| title | Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High‐Cryotolerant Rams or Extender |
| title_full | Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High‐Cryotolerant Rams or Extender |
| title_fullStr | Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High‐Cryotolerant Rams or Extender |
| title_full_unstemmed | Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High‐Cryotolerant Rams or Extender |
| title_short | Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High‐Cryotolerant Rams or Extender |
| title_sort | improving ram semen low cryotolerance by replacing the seminal plasma with that of high cryotolerant rams or extender |
| topic | cryotolerance extender ram semen seminal plasma |
| url | https://doi.org/10.1002/vms3.70381 |
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