Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells
The core proteomes of human and rat pancreatic beta cells were compared by label-free LC-MS/MS: this resulted in quantification of relative molar abundances of 707 proteins belonging to functional pathways of intermediary metabolism, protein synthesis, and cytoskeleton. Relative molar abundances wer...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2015/549818 |
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| author | G. A. Martens |
| author_facet | G. A. Martens |
| author_sort | G. A. Martens |
| collection | DOAJ |
| description | The core proteomes of human and rat pancreatic beta cells were compared by label-free LC-MS/MS: this resulted in quantification of relative molar abundances of 707 proteins belonging to functional pathways of intermediary metabolism, protein synthesis, and cytoskeleton. Relative molar abundances were conserved both within and between pathways enabling the selection of a housekeeping network for geometric normalization and the analysis of potentially relevant differential expressions. Human beta cells differed from rat beta cells in their lower level of enzymes involved in glucose sensing (MDH1, PC, and ACLY) and upregulation of lysosomal enzymes. Human cells also expressed more heat shock proteins and radical scavenging systems: apart from SOD2, they expressed high levels of H2O2-scavenger peroxiredoxin 3 (PRDX3), confirmed by microarray, Western blotting, and microscopy. Besides conferring lower susceptibility to oxidative stress to human cells PRDX3 might also play a role in physiological redox regulation as, in rat, its expression was restricted to a beta cell subset with higher metabolic glucose responsiveness. In conclusion, although their core proteomic architecture is conserved, human and rat beta cells differ in their molar expression of key enzymes involved in glucose sensing and redox control. |
| format | Article |
| id | doaj-art-bc9ceede8dfc4db1a0877adc0fa99771 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-bc9ceede8dfc4db1a0877adc0fa997712025-02-03T01:12:18ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/549818549818Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta CellsG. A. Martens0B-Probe, Diabetes Research Center, Brussels Free University (VUB), BelgiumThe core proteomes of human and rat pancreatic beta cells were compared by label-free LC-MS/MS: this resulted in quantification of relative molar abundances of 707 proteins belonging to functional pathways of intermediary metabolism, protein synthesis, and cytoskeleton. Relative molar abundances were conserved both within and between pathways enabling the selection of a housekeeping network for geometric normalization and the analysis of potentially relevant differential expressions. Human beta cells differed from rat beta cells in their lower level of enzymes involved in glucose sensing (MDH1, PC, and ACLY) and upregulation of lysosomal enzymes. Human cells also expressed more heat shock proteins and radical scavenging systems: apart from SOD2, they expressed high levels of H2O2-scavenger peroxiredoxin 3 (PRDX3), confirmed by microarray, Western blotting, and microscopy. Besides conferring lower susceptibility to oxidative stress to human cells PRDX3 might also play a role in physiological redox regulation as, in rat, its expression was restricted to a beta cell subset with higher metabolic glucose responsiveness. In conclusion, although their core proteomic architecture is conserved, human and rat beta cells differ in their molar expression of key enzymes involved in glucose sensing and redox control.http://dx.doi.org/10.1155/2015/549818 |
| spellingShingle | G. A. Martens Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells Journal of Diabetes Research |
| title | Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells |
| title_full | Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells |
| title_fullStr | Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells |
| title_full_unstemmed | Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells |
| title_short | Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells |
| title_sort | species related differences in the proteome of rat and human pancreatic beta cells |
| url | http://dx.doi.org/10.1155/2015/549818 |
| work_keys_str_mv | AT gamartens speciesrelateddifferencesintheproteomeofratandhumanpancreaticbetacells |