Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia
High consumer demand for seafood has led to the need for large-scale, reliable supply through aquaculture farming. However, bacterial infections - which can spread rapidly among the dense farming area pose a major threat to this industry. The farmers therefore often resort to extensive use of antib...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
HH Publisher
2019-04-01
|
| Series: | Progress in Microbes and Molecular Biology |
| Online Access: | https://journals.hh-publisher.com/index.php/pmmb/article/view/49 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832541435494137856 |
|---|---|
| author | Vengadesh Letchumanan Nurul-Syakima Ab Mutalib Sunny Hei Wong Kok-Gan Chan Learn-Han Lee |
| author_facet | Vengadesh Letchumanan Nurul-Syakima Ab Mutalib Sunny Hei Wong Kok-Gan Chan Learn-Han Lee |
| author_sort | Vengadesh Letchumanan |
| collection | DOAJ |
| description |
High consumer demand for seafood has led to the need for large-scale, reliable supply through aquaculture farming. However, bacterial infections - which can spread rapidly among the dense farming area pose a major threat to this industry. The farmers therefore often resort to extensive use of antibiotics, both prophylactically and therapeutically, in order to protect their stocks. The extensive use of antibiotics in aquaculture has been postulated to represent a major contributing factor in the rising incidence of antimicrobial resistant pathogenic bacteria in seafood; which may then lead to the spread of antimicrobial resistant bacteria in the environment as well as posing a significant threat to human health. This study aimed to characterize antibiotic resistance of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia. The antibiotic susceptibility of 385 V. parahaemolyticus isolates was investigated against 14 antibiotics followed by plasmid profiling and plasmid curing to determine the antibiotic mediation. A large number of isolates showed resistance to ampicillin (85%), amikacin (66.8%), and kanamycin (50.1%). A notable resistance pattern was also observed to the third generation cephalosporins (cefotaxime 55.8% and ceftazidime 34%). Only 338 V. parahaemolyticus isolates had 1-7 different plasmids and could be categorized into 27 patterns based on the number and pattern of plasmid present. Interestingly, there was no correlation between the number of plasmids and antibiotic resistant patterns seen in the isolates. The antibiotic resistance was mediated by both chromosomal and plasmid mediation among the resistant isolates. In summary, our results demonstrate that incidence of pathogenic V. parahaemolyticus in seafood in Selangor remains in relatively assuring levels, however the identification of antibiotic resistance among the isolates does rises a public health concern and warrants for continuous surveillance.
|
| format | Article |
| id | doaj-art-bc9c3cd5881c4770a26f46b8eafcf01d |
| institution | Kabale University |
| issn | 2637-1049 |
| language | English |
| publishDate | 2019-04-01 |
| publisher | HH Publisher |
| record_format | Article |
| series | Progress in Microbes and Molecular Biology |
| spelling | doaj-art-bc9c3cd5881c4770a26f46b8eafcf01d2025-02-04T08:37:15ZengHH PublisherProgress in Microbes and Molecular Biology2637-10492019-04-012110.36877/pmmb.a0000019Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, MalaysiaVengadesh LetchumananNurul-Syakima Ab MutalibSunny Hei WongKok-Gan ChanLearn-Han Lee High consumer demand for seafood has led to the need for large-scale, reliable supply through aquaculture farming. However, bacterial infections - which can spread rapidly among the dense farming area pose a major threat to this industry. The farmers therefore often resort to extensive use of antibiotics, both prophylactically and therapeutically, in order to protect their stocks. The extensive use of antibiotics in aquaculture has been postulated to represent a major contributing factor in the rising incidence of antimicrobial resistant pathogenic bacteria in seafood; which may then lead to the spread of antimicrobial resistant bacteria in the environment as well as posing a significant threat to human health. This study aimed to characterize antibiotic resistance of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia. The antibiotic susceptibility of 385 V. parahaemolyticus isolates was investigated against 14 antibiotics followed by plasmid profiling and plasmid curing to determine the antibiotic mediation. A large number of isolates showed resistance to ampicillin (85%), amikacin (66.8%), and kanamycin (50.1%). A notable resistance pattern was also observed to the third generation cephalosporins (cefotaxime 55.8% and ceftazidime 34%). Only 338 V. parahaemolyticus isolates had 1-7 different plasmids and could be categorized into 27 patterns based on the number and pattern of plasmid present. Interestingly, there was no correlation between the number of plasmids and antibiotic resistant patterns seen in the isolates. The antibiotic resistance was mediated by both chromosomal and plasmid mediation among the resistant isolates. In summary, our results demonstrate that incidence of pathogenic V. parahaemolyticus in seafood in Selangor remains in relatively assuring levels, however the identification of antibiotic resistance among the isolates does rises a public health concern and warrants for continuous surveillance. https://journals.hh-publisher.com/index.php/pmmb/article/view/49 |
| spellingShingle | Vengadesh Letchumanan Nurul-Syakima Ab Mutalib Sunny Hei Wong Kok-Gan Chan Learn-Han Lee Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia Progress in Microbes and Molecular Biology |
| title | Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia |
| title_full | Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia |
| title_fullStr | Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia |
| title_full_unstemmed | Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia |
| title_short | Determination of antibiotic resistance patterns of Vibrio parahaemolyticus from shrimp and shellfish in Selangor, Malaysia |
| title_sort | determination of antibiotic resistance patterns of vibrio parahaemolyticus from shrimp and shellfish in selangor malaysia |
| url | https://journals.hh-publisher.com/index.php/pmmb/article/view/49 |
| work_keys_str_mv | AT vengadeshletchumanan determinationofantibioticresistancepatternsofvibrioparahaemolyticusfromshrimpandshellfishinselangormalaysia AT nurulsyakimaabmutalib determinationofantibioticresistancepatternsofvibrioparahaemolyticusfromshrimpandshellfishinselangormalaysia AT sunnyheiwong determinationofantibioticresistancepatternsofvibrioparahaemolyticusfromshrimpandshellfishinselangormalaysia AT kokganchan determinationofantibioticresistancepatternsofvibrioparahaemolyticusfromshrimpandshellfishinselangormalaysia AT learnhanlee determinationofantibioticresistancepatternsofvibrioparahaemolyticusfromshrimpandshellfishinselangormalaysia |