Molecular detection by some virulence genes of Salmonella enterica subsp. enterica isolated from the stool of children with diarrhea
Diarrhea is a real disease in childhood which could cause death. Therefore, this study was conducted to isolate Salmonella from 350 stool samples taken from children under five years in age, suffering from diarrhea during the period from March 2019 to March 2020 in Tikrit city / Iraq. The results s...
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University of Baghdad, College of Science for Women
2023-10-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/6806 |
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| author | Mohammed Nadhir Maaroof Ahmed Hussein Dhayea |
| author_facet | Mohammed Nadhir Maaroof Ahmed Hussein Dhayea |
| author_sort | Mohammed Nadhir Maaroof |
| collection | DOAJ |
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Diarrhea is a real disease in childhood which could cause death. Therefore, this study was conducted to isolate Salmonella from 350 stool samples taken from children under five years in age, suffering from diarrhea during the period from March 2019 to March 2020 in Tikrit city / Iraq. The results showed the possibility to isolate ten isolates of Salmonella enterica subsp. Enterica, an infection rate, represents 2.875% of the total rate of patients who suffer from diarrhea. The virulence genes were investigated for ten isolates of S. enterica subsp. enterica, the result is that all isolates possessed the genes stn, invA, lpfA with an appearance percentage of 100%, while the percentage of appearance of pefA and fimH genes was 80% and 60%, respectively. All these isolates as Iraqi detection have been submitted to NCBI then accepted as ten Iraqi strains were in NCBI. All strains were concordance of 99-100% with the Salmonella enterica subsp. enterica Strains of Taiwan (CP085821.1), the United Kingdom (OU943338.1), China (CP085699.1), South Korea (CP077760.1), Pakistan (OK035700.1) and Hong Kong (CP0823381.1) in addition to four US states.
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| format | Article |
| id | doaj-art-bc9b060eeae84ce6953473e35f500ecb |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-bc9b060eeae84ce6953473e35f500ecb2025-08-20T03:39:26ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862023-10-0120510.21123/bsj.2023.6806Molecular detection by some virulence genes of Salmonella enterica subsp. enterica isolated from the stool of children with diarrheaMohammed Nadhir Maaroof0Ahmed Hussein Dhayea1Deptatment of Biology, College of Education for Pure Science, Tikrit University, Tikrit, Iraq.Salah Al-Din Education Directorate, Ministry of Education. Diarrhea is a real disease in childhood which could cause death. Therefore, this study was conducted to isolate Salmonella from 350 stool samples taken from children under five years in age, suffering from diarrhea during the period from March 2019 to March 2020 in Tikrit city / Iraq. The results showed the possibility to isolate ten isolates of Salmonella enterica subsp. Enterica, an infection rate, represents 2.875% of the total rate of patients who suffer from diarrhea. The virulence genes were investigated for ten isolates of S. enterica subsp. enterica, the result is that all isolates possessed the genes stn, invA, lpfA with an appearance percentage of 100%, while the percentage of appearance of pefA and fimH genes was 80% and 60%, respectively. All these isolates as Iraqi detection have been submitted to NCBI then accepted as ten Iraqi strains were in NCBI. All strains were concordance of 99-100% with the Salmonella enterica subsp. enterica Strains of Taiwan (CP085821.1), the United Kingdom (OU943338.1), China (CP085699.1), South Korea (CP077760.1), Pakistan (OK035700.1) and Hong Kong (CP0823381.1) in addition to four US states. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/6806Diarrhea, Salmonella enterica subsp enterica, Virulence factors genes, 16srRNA gene, Phylogenetic tree |
| spellingShingle | Mohammed Nadhir Maaroof Ahmed Hussein Dhayea Molecular detection by some virulence genes of Salmonella enterica subsp. enterica isolated from the stool of children with diarrhea مجلة بغداد للعلوم Diarrhea, Salmonella enterica subsp enterica, Virulence factors genes, 16srRNA gene, Phylogenetic tree |
| title | Molecular detection by some virulence genes of Salmonella enterica subsp. enterica isolated from the stool of children with diarrhea |
| title_full | Molecular detection by some virulence genes of Salmonella enterica subsp. enterica isolated from the stool of children with diarrhea |
| title_fullStr | Molecular detection by some virulence genes of Salmonella enterica subsp. enterica isolated from the stool of children with diarrhea |
| title_full_unstemmed | Molecular detection by some virulence genes of Salmonella enterica subsp. enterica isolated from the stool of children with diarrhea |
| title_short | Molecular detection by some virulence genes of Salmonella enterica subsp. enterica isolated from the stool of children with diarrhea |
| title_sort | molecular detection by some virulence genes of salmonella enterica subsp enterica isolated from the stool of children with diarrhea |
| topic | Diarrhea, Salmonella enterica subsp enterica, Virulence factors genes, 16srRNA gene, Phylogenetic tree |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/6806 |
| work_keys_str_mv | AT mohammednadhirmaaroof moleculardetectionbysomevirulencegenesofsalmonellaentericasubspentericaisolatedfromthestoolofchildrenwithdiarrhea AT ahmedhusseindhayea moleculardetectionbysomevirulencegenesofsalmonellaentericasubspentericaisolatedfromthestoolofchildrenwithdiarrhea |