A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability

MicroRNAs (miRNAs) are short (∼22 nucleotides), non-coding RNA molecules that post-transcriptionally regulate gene expression. As the miRNA field is still in its relative infancy, there is currently a lack of consensus regarding optimal methodologies for miRNA quantification, data analysis and data...

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Bibliographic Details
Main Authors: Nicholas Redshaw, Timothy Wilkes, Alexandra Whale, Simon Cowen, Jim Huggett, Carole A. Foy
Format: Article
Language:English
Published: Taylor & Francis Group 2013-03-01
Series:BioTechniques
Subjects:
MicroRNAs
RT-qPCR
short RNA enrichment
Online Access:https://www.future-science.com/doi/10.2144/000114002
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