Analysis of CYP701A1 genes in gossypium species and functional characterization through gene silencing
Gibberellins (GA) are known to play crucial roles in various aspects of plant growth and development. The cytochrome P450 enzyme family is recognized for its significance in plant metabolic processes. Specifically, CYP701s, a subgroup of CYP71, encode ent-kaurene oxidase in the gibberellin synthesis...
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Elsevier
2025-02-01
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| Series: | Crop Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772899424000302 |
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| author | Zhao Liang Di Jiachun Guo Qi Xu Zhenzhen Zhao Jun Xu Peng Xu Jianwen Liu Jianguang Shen Xinlian Chen Xusheng |
| author_facet | Zhao Liang Di Jiachun Guo Qi Xu Zhenzhen Zhao Jun Xu Peng Xu Jianwen Liu Jianguang Shen Xinlian Chen Xusheng |
| author_sort | Zhao Liang |
| collection | DOAJ |
| description | Gibberellins (GA) are known to play crucial roles in various aspects of plant growth and development. The cytochrome P450 enzyme family is recognized for its significance in plant metabolic processes. Specifically, CYP701s, a subgroup of CYP71, encode ent-kaurene oxidase in the gibberellin synthesis pathway. In this study, we analyzed genomic data from 30 Gossypium species, including nine allotetraploid genomes (AD1-AD7, with two each for AD1 and AD2), 21 diploid genomes (A-G, K, with two A-genomes and 12 D-genomes), and Gossypioides kirkii genome as an outgroup for evolutionary analysis, totaling 31 genomes. Subsequently, 40 CYP701A1 genes were identified from various genomes and conducted a comprehensive analysis of their structure and evolution. Virus-induced gene silencing (VIGS) technology was utilized to knock out the GhCYP701A1 gene in Gossypium hirsutum ac TM-1. Subsequent analysis revealed changes in hormone content, with decreased gibberellin levels and notable increases in auxin, cytokinin, and jasmonic acid contents. Conversely, salicylic acid content decreased, while the precursor for ethylene synthesis, 1-aminocyclopropane-1-carboxylic acid (ACC), remained relatively stable. Transcriptome analysis of the gene silencing plants identified 15,962 differentially expressed genes, including 8376 upregulated and 7586 downregulated genes. Enrichment analysis through KEGG pathway highlighted ‘Plant hormone signal transduction’ as a prominent pathway with 234 differentially expressed genes. The study provided insights into the function and regulatory network of the gene. |
| format | Article |
| id | doaj-art-bc87706791f646e2a69cf0fc0d305506 |
| institution | Kabale University |
| issn | 2772-8994 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Crop Design |
| spelling | doaj-art-bc87706791f646e2a69cf0fc0d3055062025-01-26T05:05:23ZengElsevierCrop Design2772-89942025-02-0141100081Analysis of CYP701A1 genes in gossypium species and functional characterization through gene silencingZhao Liang0Di Jiachun1Guo Qi2Xu Zhenzhen3Zhao Jun4Xu Peng5Xu Jianwen6Liu Jianguang7Shen Xinlian8Chen Xusheng9Key Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaInstitute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China; Corresponding author.Gibberellins (GA) are known to play crucial roles in various aspects of plant growth and development. The cytochrome P450 enzyme family is recognized for its significance in plant metabolic processes. Specifically, CYP701s, a subgroup of CYP71, encode ent-kaurene oxidase in the gibberellin synthesis pathway. In this study, we analyzed genomic data from 30 Gossypium species, including nine allotetraploid genomes (AD1-AD7, with two each for AD1 and AD2), 21 diploid genomes (A-G, K, with two A-genomes and 12 D-genomes), and Gossypioides kirkii genome as an outgroup for evolutionary analysis, totaling 31 genomes. Subsequently, 40 CYP701A1 genes were identified from various genomes and conducted a comprehensive analysis of their structure and evolution. Virus-induced gene silencing (VIGS) technology was utilized to knock out the GhCYP701A1 gene in Gossypium hirsutum ac TM-1. Subsequent analysis revealed changes in hormone content, with decreased gibberellin levels and notable increases in auxin, cytokinin, and jasmonic acid contents. Conversely, salicylic acid content decreased, while the precursor for ethylene synthesis, 1-aminocyclopropane-1-carboxylic acid (ACC), remained relatively stable. Transcriptome analysis of the gene silencing plants identified 15,962 differentially expressed genes, including 8376 upregulated and 7586 downregulated genes. Enrichment analysis through KEGG pathway highlighted ‘Plant hormone signal transduction’ as a prominent pathway with 234 differentially expressed genes. The study provided insights into the function and regulatory network of the gene.http://www.sciencedirect.com/science/article/pii/S2772899424000302CottonCytochrome P450GibberellinsCYP701Regulatory network |
| spellingShingle | Zhao Liang Di Jiachun Guo Qi Xu Zhenzhen Zhao Jun Xu Peng Xu Jianwen Liu Jianguang Shen Xinlian Chen Xusheng Analysis of CYP701A1 genes in gossypium species and functional characterization through gene silencing Crop Design Cotton Cytochrome P450 Gibberellins CYP701 Regulatory network |
| title | Analysis of CYP701A1 genes in gossypium species and functional characterization through gene silencing |
| title_full | Analysis of CYP701A1 genes in gossypium species and functional characterization through gene silencing |
| title_fullStr | Analysis of CYP701A1 genes in gossypium species and functional characterization through gene silencing |
| title_full_unstemmed | Analysis of CYP701A1 genes in gossypium species and functional characterization through gene silencing |
| title_short | Analysis of CYP701A1 genes in gossypium species and functional characterization through gene silencing |
| title_sort | analysis of cyp701a1 genes in gossypium species and functional characterization through gene silencing |
| topic | Cotton Cytochrome P450 Gibberellins CYP701 Regulatory network |
| url | http://www.sciencedirect.com/science/article/pii/S2772899424000302 |
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