Targeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA
Abstract Cisplatin (DDP) is a common therapeutic option for non‐small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. Therefore, identifying novel biomarkers to improve the diagnosis and treatment of NSCLC is important. Ubiquitin‐specific protease (USP14)...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-04-01
|
| Series: | MedComm |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mco2.237 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589935834562560 |
|---|---|
| author | Xinmin Zhao Xianghua Wu Huijie Wang Songtao Lai Jialei Wang |
| author_facet | Xinmin Zhao Xianghua Wu Huijie Wang Songtao Lai Jialei Wang |
| author_sort | Xinmin Zhao |
| collection | DOAJ |
| description | Abstract Cisplatin (DDP) is a common therapeutic option for non‐small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. Therefore, identifying novel biomarkers to improve the diagnosis and treatment of NSCLC is important. Ubiquitin‐specific protease (USP14) is involved in various pathological conditions including cancer; however, the role of USP14 in NSCLC remains elusive. The SELEX technology was used to identify aptamers that specifically recognize DDP‐resistant lung cancer cells and couple them with nano‐zinc (zinc hydroxide, Zn(OH)2) carriers. USP14 levels were higher in DDP‐resistant lung cancer compared to DDP‐sensitive lung cancer. The survival rate of lung cancer patients with increased USP14 expression was significantly lower than the survival rate of patients with low USP14 expression. Silencing USP14 increased the tumor antagonistic action of DDP in A549 cisplatin‐resistant (A549/DDP) cells, while USP14 overexpression decreased the antagonist effects. Aptamer‐targeted nano‐zinc carriers were loaded with USP14 siRNA to target DDP‐resistant lung cancer cells. Aptamer‐targeted nano‐zinc carriers containing USP14 siRNA increased the antitumor effects of DDP in A549/DDP cells and mice bearing A549/DDP cells. These results indicate that aptamer‐guided nano‐zinc carriers may be a potent carrier for the precise treatment of drug‐resistant tumors. |
| format | Article |
| id | doaj-art-bc775a4328ff42739899a780b53d9925 |
| institution | Kabale University |
| issn | 2688-2663 |
| language | English |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | MedComm |
| spelling | doaj-art-bc775a4328ff42739899a780b53d99252025-01-24T05:36:29ZengWileyMedComm2688-26632023-04-0142n/an/a10.1002/mco2.237Targeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNAXinmin Zhao0Xianghua Wu1Huijie Wang2Songtao Lai3Jialei Wang4Department of Thoracic Medical Oncology Fudan University Shanghai Cancer Center Shanghai ChinaDepartment of Thoracic Medical Oncology Fudan University Shanghai Cancer Center Shanghai ChinaDepartment of Thoracic Medical Oncology Fudan University Shanghai Cancer Center Shanghai ChinaDepartment of Oncology Shanghai Medical College, Fudan University Shanghai ChinaDepartment of Thoracic Medical Oncology Fudan University Shanghai Cancer Center Shanghai ChinaAbstract Cisplatin (DDP) is a common therapeutic option for non‐small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. Therefore, identifying novel biomarkers to improve the diagnosis and treatment of NSCLC is important. Ubiquitin‐specific protease (USP14) is involved in various pathological conditions including cancer; however, the role of USP14 in NSCLC remains elusive. The SELEX technology was used to identify aptamers that specifically recognize DDP‐resistant lung cancer cells and couple them with nano‐zinc (zinc hydroxide, Zn(OH)2) carriers. USP14 levels were higher in DDP‐resistant lung cancer compared to DDP‐sensitive lung cancer. The survival rate of lung cancer patients with increased USP14 expression was significantly lower than the survival rate of patients with low USP14 expression. Silencing USP14 increased the tumor antagonistic action of DDP in A549 cisplatin‐resistant (A549/DDP) cells, while USP14 overexpression decreased the antagonist effects. Aptamer‐targeted nano‐zinc carriers were loaded with USP14 siRNA to target DDP‐resistant lung cancer cells. Aptamer‐targeted nano‐zinc carriers containing USP14 siRNA increased the antitumor effects of DDP in A549/DDP cells and mice bearing A549/DDP cells. These results indicate that aptamer‐guided nano‐zinc carriers may be a potent carrier for the precise treatment of drug‐resistant tumors.https://doi.org/10.1002/mco2.237cisplatin‐resistancenano‐zinc carriersnon‐small cell lung cancersmall interfering RNAtargeted therapy |
| spellingShingle | Xinmin Zhao Xianghua Wu Huijie Wang Songtao Lai Jialei Wang Targeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA MedComm cisplatin‐resistance nano‐zinc carriers non‐small cell lung cancer small interfering RNA targeted therapy |
| title | Targeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA |
| title_full | Targeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA |
| title_fullStr | Targeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA |
| title_full_unstemmed | Targeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA |
| title_short | Targeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA |
| title_sort | targeted therapy for cisplatin resistant lung cancer via aptamer guided nano zinc carriers containing usp14 sirna |
| topic | cisplatin‐resistance nano‐zinc carriers non‐small cell lung cancer small interfering RNA targeted therapy |
| url | https://doi.org/10.1002/mco2.237 |
| work_keys_str_mv | AT xinminzhao targetedtherapyforcisplatinresistantlungcancerviaaptamerguidednanozinccarrierscontainingusp14sirna AT xianghuawu targetedtherapyforcisplatinresistantlungcancerviaaptamerguidednanozinccarrierscontainingusp14sirna AT huijiewang targetedtherapyforcisplatinresistantlungcancerviaaptamerguidednanozinccarrierscontainingusp14sirna AT songtaolai targetedtherapyforcisplatinresistantlungcancerviaaptamerguidednanozinccarrierscontainingusp14sirna AT jialeiwang targetedtherapyforcisplatinresistantlungcancerviaaptamerguidednanozinccarrierscontainingusp14sirna |