Salted egg white derived-peptides VF-4 and DR-8 improve DSS-induced colitis in mice by inhibiting NF-κB/MAPK/PI3K-AKT signaling pathways and modulating gut microbiota composition
Inflammation underlies many chronic diseases, and inflammatory bowel disease (IBD) is a condition characterized by long-term inflammation of the gut. Egg whites have been shown to contain many beneficial active substances. Therefore, we obtained 2 peptides from salted egg white: Val-Val-His-Phe (VF-...
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Tsinghua University Press
2025-06-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2024.9250136 |
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| author | Na Zhou Na Wu Yao Yao Shuping Chen Mingsheng Xu Zhongping Yin Yan Zhao Yonggang Tu |
| author_facet | Na Zhou Na Wu Yao Yao Shuping Chen Mingsheng Xu Zhongping Yin Yan Zhao Yonggang Tu |
| author_sort | Na Zhou |
| collection | DOAJ |
| description | Inflammation underlies many chronic diseases, and inflammatory bowel disease (IBD) is a condition characterized by long-term inflammation of the gut. Egg whites have been shown to contain many beneficial active substances. Therefore, we obtained 2 peptides from salted egg white: Val-Val-His-Phe (VF-4) and Asp-Thr-Gln-Ala-Met-Pro-Phe-Arg (DR-8). The sodium dextran sulfate (DSS)-induced mice colitis model was used to evaluate its regulatory effect on colitis in vivo. The results showed that VF-4 and DR-8 improved the clinical symptoms of DSS-induced colitis, attenuated colon tissue damage, inhibited the activation of nuclear factor kappa-B (NF-κB)/mitogen-activated protein kinase (MAPK)/phosphoinositide 3-kinase-Akt (PI3K-AKT) signaling pathways, and inhibited the expression of inflammatory cytokines. 16S rRNA gene sequencing showed that VF-4 and DR-8 administration increased the relative abundance of intestinal beneficial bacteria including Lactobacillus, Blautia, and down-regulated the relative abundance of inflammation-related bacteria including Acinetobacter, Lachnospiraceae_NK4A136_group, Klebsiella. Moreover, the degree of correlation between pro-inflammatory cytokines and microbiota was as follows: interleukin-6 (IL-6) > tumor necrosis factor-α (TNF-α) > interleukin-1β (IL-1β) > interferon-γ (IFN-γ). In conclusion, this study suggests that salted egg white peptides VF-4 and DR-8 have a significant anti-inflammatory effect in vivo. It also provides a strategy for the treatment of IBD and a new way for the high-value utilization of salted egg white. |
| format | Article |
| id | doaj-art-bc6dbeefecfd46ebab42a23f1c5d6ad6 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-bc6dbeefecfd46ebab42a23f1c5d6ad62025-08-20T03:59:44ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-06-01146925013610.26599/FSHW.2024.9250136Salted egg white derived-peptides VF-4 and DR-8 improve DSS-induced colitis in mice by inhibiting NF-κB/MAPK/PI3K-AKT signaling pathways and modulating gut microbiota compositionNa Zhou0Na Wu1Yao Yao2Shuping Chen3Mingsheng Xu4Zhongping Yin5Yan Zhao6Yonggang Tu7Jiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaInflammation underlies many chronic diseases, and inflammatory bowel disease (IBD) is a condition characterized by long-term inflammation of the gut. Egg whites have been shown to contain many beneficial active substances. Therefore, we obtained 2 peptides from salted egg white: Val-Val-His-Phe (VF-4) and Asp-Thr-Gln-Ala-Met-Pro-Phe-Arg (DR-8). The sodium dextran sulfate (DSS)-induced mice colitis model was used to evaluate its regulatory effect on colitis in vivo. The results showed that VF-4 and DR-8 improved the clinical symptoms of DSS-induced colitis, attenuated colon tissue damage, inhibited the activation of nuclear factor kappa-B (NF-κB)/mitogen-activated protein kinase (MAPK)/phosphoinositide 3-kinase-Akt (PI3K-AKT) signaling pathways, and inhibited the expression of inflammatory cytokines. 16S rRNA gene sequencing showed that VF-4 and DR-8 administration increased the relative abundance of intestinal beneficial bacteria including Lactobacillus, Blautia, and down-regulated the relative abundance of inflammation-related bacteria including Acinetobacter, Lachnospiraceae_NK4A136_group, Klebsiella. Moreover, the degree of correlation between pro-inflammatory cytokines and microbiota was as follows: interleukin-6 (IL-6) > tumor necrosis factor-α (TNF-α) > interleukin-1β (IL-1β) > interferon-γ (IFN-γ). In conclusion, this study suggests that salted egg white peptides VF-4 and DR-8 have a significant anti-inflammatory effect in vivo. It also provides a strategy for the treatment of IBD and a new way for the high-value utilization of salted egg white.https://www.sciopen.com/article/10.26599/FSHW.2024.9250136salted egg whitepeptidescolitisnf-κb/mapk/pi3k-akt pathwaysintestinal flora |
| spellingShingle | Na Zhou Na Wu Yao Yao Shuping Chen Mingsheng Xu Zhongping Yin Yan Zhao Yonggang Tu Salted egg white derived-peptides VF-4 and DR-8 improve DSS-induced colitis in mice by inhibiting NF-κB/MAPK/PI3K-AKT signaling pathways and modulating gut microbiota composition Food Science and Human Wellness salted egg white peptides colitis nf-κb/mapk/pi3k-akt pathways intestinal flora |
| title | Salted egg white derived-peptides VF-4 and DR-8 improve DSS-induced colitis in mice by inhibiting NF-κB/MAPK/PI3K-AKT signaling pathways and modulating gut microbiota composition |
| title_full | Salted egg white derived-peptides VF-4 and DR-8 improve DSS-induced colitis in mice by inhibiting NF-κB/MAPK/PI3K-AKT signaling pathways and modulating gut microbiota composition |
| title_fullStr | Salted egg white derived-peptides VF-4 and DR-8 improve DSS-induced colitis in mice by inhibiting NF-κB/MAPK/PI3K-AKT signaling pathways and modulating gut microbiota composition |
| title_full_unstemmed | Salted egg white derived-peptides VF-4 and DR-8 improve DSS-induced colitis in mice by inhibiting NF-κB/MAPK/PI3K-AKT signaling pathways and modulating gut microbiota composition |
| title_short | Salted egg white derived-peptides VF-4 and DR-8 improve DSS-induced colitis in mice by inhibiting NF-κB/MAPK/PI3K-AKT signaling pathways and modulating gut microbiota composition |
| title_sort | salted egg white derived peptides vf 4 and dr 8 improve dss induced colitis in mice by inhibiting nf κb mapk pi3k akt signaling pathways and modulating gut microbiota composition |
| topic | salted egg white peptides colitis nf-κb/mapk/pi3k-akt pathways intestinal flora |
| url | https://www.sciopen.com/article/10.26599/FSHW.2024.9250136 |
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