Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract
Agents of immunomodulation are compounds that have the ability to balance the immune system throughout the body. The neem plant is one of the herbal plants that is often used as traditional medicine. Neem leaves (Azadirachta indica A. Juss) contain antioxidants that have the potential to be antiinfl...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Indonesian |
| Published: |
Universitas Gadjah Mada
2024-12-01
|
| Series: | Jurnal Sain Veteriner |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/jsv/article/view/92377 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594235828731904 |
|---|---|
| author | Putri Lestari Ningtias Teguh Suprihatih Agung Janika Sitasiwi |
| author_facet | Putri Lestari Ningtias Teguh Suprihatih Agung Janika Sitasiwi |
| author_sort | Putri Lestari Ningtias |
| collection | DOAJ |
| description | Agents of immunomodulation are compounds that have the ability to balance the immune system throughout the body. The neem plant is one of the herbal plants that is often used as traditional medicine. Neem leaves (Azadirachta indica A. Juss) contain antioxidants that have the potential to be antiinflammatory, antiviral, and as an immunomodulatory agent. The constraint in giving medicine orally is the low bioavailability of drugs and the distribution of active compounds of herbal plants. This problem can be solved by packaging herbal plant extracts in nanochitosan. The purpose of this study was to analyze the effect of induced of a nanochitosan ethanol extract of neem leaves on the histological structure of the spleen tissue of female Wistar white rats. This study used a randomized design (CRD) which was divided into 3 treatment groups with 4 repetitions. Treatments include control (P0), an ethanol extract of neem leaves 2 ml/head/day (P1), and nanochitosan preparation of neem leaves 2 ml/head/day (P2). The parameters measured were the diameter of white pulp, the diameter of germinal centre, the area of white pulp, and the area of germinal centre in the spleen. Data on the diameter and the area of the white pulp and germinal centre were analyzed statistically parametrically with ANOVA with a 95% level of confidence, if the data were not normally distributed, the Kruskall Wallis nonparametric test was carried out. Data that were significantly different were carried out by Duncan’s further test. The results showed that the treatment of nanochitosan preparations of neem leaf ethanol extract had a significant difference in the diameter of the white pulp and germinal centre as well as the area of the white pulp and germinal centre (P<0,05). The conclusion of this study was that the nanochitosan preparation ethanol extract of neem leaves at a dose of 14 mg/kg BB can increase the diameter and the area of the white pulp and germinal centre. |
| format | Article |
| id | doaj-art-bc62d0cebcc642d2839e5a035a79353a |
| institution | Kabale University |
| issn | 2407-3733 |
| language | Indonesian |
| publishDate | 2024-12-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Sain Veteriner |
| spelling | doaj-art-bc62d0cebcc642d2839e5a035a79353a2025-01-20T02:11:56ZindUniversitas Gadjah MadaJurnal Sain Veteriner2407-37332024-12-0142344845610.22146/jsv.9237736908Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol ExtractPutri Lestari Ningtias0Teguh Suprihatih1Agung Janika Sitasiwi2Diponegoro UniversityDepartemen Biologi, Universitas Diponegoro, Semarang, Jawa Tengah, IndonesiaDepartemen Biologi, Universitas Diponegoro, Semarang, Jawa Tengah, IndonesiaAgents of immunomodulation are compounds that have the ability to balance the immune system throughout the body. The neem plant is one of the herbal plants that is often used as traditional medicine. Neem leaves (Azadirachta indica A. Juss) contain antioxidants that have the potential to be antiinflammatory, antiviral, and as an immunomodulatory agent. The constraint in giving medicine orally is the low bioavailability of drugs and the distribution of active compounds of herbal plants. This problem can be solved by packaging herbal plant extracts in nanochitosan. The purpose of this study was to analyze the effect of induced of a nanochitosan ethanol extract of neem leaves on the histological structure of the spleen tissue of female Wistar white rats. This study used a randomized design (CRD) which was divided into 3 treatment groups with 4 repetitions. Treatments include control (P0), an ethanol extract of neem leaves 2 ml/head/day (P1), and nanochitosan preparation of neem leaves 2 ml/head/day (P2). The parameters measured were the diameter of white pulp, the diameter of germinal centre, the area of white pulp, and the area of germinal centre in the spleen. Data on the diameter and the area of the white pulp and germinal centre were analyzed statistically parametrically with ANOVA with a 95% level of confidence, if the data were not normally distributed, the Kruskall Wallis nonparametric test was carried out. Data that were significantly different were carried out by Duncan’s further test. The results showed that the treatment of nanochitosan preparations of neem leaf ethanol extract had a significant difference in the diameter of the white pulp and germinal centre as well as the area of the white pulp and germinal centre (P<0,05). The conclusion of this study was that the nanochitosan preparation ethanol extract of neem leaves at a dose of 14 mg/kg BB can increase the diameter and the area of the white pulp and germinal centre.https://jurnal.ugm.ac.id/jsv/article/view/92377nanochitosanwhite pulp, germinal centre |
| spellingShingle | Putri Lestari Ningtias Teguh Suprihatih Agung Janika Sitasiwi Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract Jurnal Sain Veteriner nanochitosan white pulp, germinal centre |
| title | Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract |
| title_full | Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract |
| title_fullStr | Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract |
| title_full_unstemmed | Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract |
| title_short | Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract |
| title_sort | histological structure of spleen white rat rattus norvegicus l after induced of nanochitosan preparation of neem leaf azadirachta indica a juss ethanol extract |
| topic | nanochitosan white pulp, germinal centre |
| url | https://jurnal.ugm.ac.id/jsv/article/view/92377 |
| work_keys_str_mv | AT putrilestariningtias histologicalstructureofspleenwhiteratrattusnorvegicuslafterinducedofnanochitosanpreparationofneemleafazadirachtaindicaajussethanolextract AT teguhsuprihatih histologicalstructureofspleenwhiteratrattusnorvegicuslafterinducedofnanochitosanpreparationofneemleafazadirachtaindicaajussethanolextract AT agungjanikasitasiwi histologicalstructureofspleenwhiteratrattusnorvegicuslafterinducedofnanochitosanpreparationofneemleafazadirachtaindicaajussethanolextract |