Maternal Diet Supplementation with n-6/n-3 Essential Fatty Acids in a 1.2 : 1.0 Ratio Attenuates Metabolic Dysfunction in MSG-Induced Obese Mice
Essential polyunsaturated fatty acids (PUFAs) prevent cardiometabolic diseases. We aimed to study whether a diet supplemented with a mixture of n-6/n-3 PUFAs, during perinatal life, attenuates outcomes of long-term metabolic dysfunction in prediabetic and obese mice. Seventy-day-old virgin female mi...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2016/9242319 |
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| author | Josiane Morais Martin Rosiane Aparecida Miranda Luiz Felipe Barella Kesia Palma-Rigo Vander Silva Alves Gabriel Sergio Fabricio Audrei Pavanello Claudinéia Conationi da Silva Franco Tatiane Aparecida Ribeiro Jesuí Vergílio Visentainer Elton Guntendeorfer Banafé Clayton Antunes Martin Paulo Cezar de Freitas Mathias Júlio Cezar de Oliveira |
| author_facet | Josiane Morais Martin Rosiane Aparecida Miranda Luiz Felipe Barella Kesia Palma-Rigo Vander Silva Alves Gabriel Sergio Fabricio Audrei Pavanello Claudinéia Conationi da Silva Franco Tatiane Aparecida Ribeiro Jesuí Vergílio Visentainer Elton Guntendeorfer Banafé Clayton Antunes Martin Paulo Cezar de Freitas Mathias Júlio Cezar de Oliveira |
| author_sort | Josiane Morais Martin |
| collection | DOAJ |
| description | Essential polyunsaturated fatty acids (PUFAs) prevent cardiometabolic diseases. We aimed to study whether a diet supplemented with a mixture of n-6/n-3 PUFAs, during perinatal life, attenuates outcomes of long-term metabolic dysfunction in prediabetic and obese mice. Seventy-day-old virgin female mice were mated. From the conception day, dams were fed a diet supplemented with sunflower oil and flaxseed powder (containing an n-6/n-3 PUFAs ratio of 1.2 : 1.0) throughout pregnancy and lactation, while control dams received a commercial diet. Newborn mice were treated with monosodium L-glutamate (MSG, 4 mg g−1 body weight per day) for the first 5 days of age. A batch of weaned pups was sacrificed to quantify the brain and pancreas total lipids; another batch were fed a commercial diet until 90 days of age, where glucose homeostasis and glucose-induced insulin secretion (GIIS) as well as retroperitoneal fat and Lee index were assessed. MSG-treated mice developed obesity, glucose intolerance, insulin resistance, pancreatic islet dysfunction, and higher fat stores. Maternal flaxseed diet-supplementation decreased n-6/n-3 PUFAs ratio in the brain and pancreas and blocked glucose intolerance, insulin resistance, GIIS impairment, and obesity development. The n-6/n-3 essential PUFAs in a ratio of 1.2 : 1.0 supplemented in maternal diet during pregnancy and lactation prevent metabolic dysfunction in MSG-obesity model. |
| format | Article |
| id | doaj-art-bc42161adc6941ef9ca578bdede15083 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-bc42161adc6941ef9ca578bdede150832025-02-03T01:11:27ZengWileyInternational Journal of Endocrinology1687-83371687-83452016-01-01201610.1155/2016/92423199242319Maternal Diet Supplementation with n-6/n-3 Essential Fatty Acids in a 1.2 : 1.0 Ratio Attenuates Metabolic Dysfunction in MSG-Induced Obese MiceJosiane Morais Martin0Rosiane Aparecida Miranda1Luiz Felipe Barella2Kesia Palma-Rigo3Vander Silva Alves4Gabriel Sergio Fabricio5Audrei Pavanello6Claudinéia Conationi da Silva Franco7Tatiane Aparecida Ribeiro8Jesuí Vergílio Visentainer9Elton Guntendeorfer Banafé10Clayton Antunes Martin11Paulo Cezar de Freitas Mathias12Júlio Cezar de Oliveira13Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Química, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Química, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilUniversidade Tecnológica Federal do Paraná, 85902-490 Toledo, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, PR, BrazilEssential polyunsaturated fatty acids (PUFAs) prevent cardiometabolic diseases. We aimed to study whether a diet supplemented with a mixture of n-6/n-3 PUFAs, during perinatal life, attenuates outcomes of long-term metabolic dysfunction in prediabetic and obese mice. Seventy-day-old virgin female mice were mated. From the conception day, dams were fed a diet supplemented with sunflower oil and flaxseed powder (containing an n-6/n-3 PUFAs ratio of 1.2 : 1.0) throughout pregnancy and lactation, while control dams received a commercial diet. Newborn mice were treated with monosodium L-glutamate (MSG, 4 mg g−1 body weight per day) for the first 5 days of age. A batch of weaned pups was sacrificed to quantify the brain and pancreas total lipids; another batch were fed a commercial diet until 90 days of age, where glucose homeostasis and glucose-induced insulin secretion (GIIS) as well as retroperitoneal fat and Lee index were assessed. MSG-treated mice developed obesity, glucose intolerance, insulin resistance, pancreatic islet dysfunction, and higher fat stores. Maternal flaxseed diet-supplementation decreased n-6/n-3 PUFAs ratio in the brain and pancreas and blocked glucose intolerance, insulin resistance, GIIS impairment, and obesity development. The n-6/n-3 essential PUFAs in a ratio of 1.2 : 1.0 supplemented in maternal diet during pregnancy and lactation prevent metabolic dysfunction in MSG-obesity model.http://dx.doi.org/10.1155/2016/9242319 |
| spellingShingle | Josiane Morais Martin Rosiane Aparecida Miranda Luiz Felipe Barella Kesia Palma-Rigo Vander Silva Alves Gabriel Sergio Fabricio Audrei Pavanello Claudinéia Conationi da Silva Franco Tatiane Aparecida Ribeiro Jesuí Vergílio Visentainer Elton Guntendeorfer Banafé Clayton Antunes Martin Paulo Cezar de Freitas Mathias Júlio Cezar de Oliveira Maternal Diet Supplementation with n-6/n-3 Essential Fatty Acids in a 1.2 : 1.0 Ratio Attenuates Metabolic Dysfunction in MSG-Induced Obese Mice International Journal of Endocrinology |
| title | Maternal Diet Supplementation with n-6/n-3 Essential Fatty Acids in a 1.2 : 1.0 Ratio Attenuates Metabolic Dysfunction in MSG-Induced Obese Mice |
| title_full | Maternal Diet Supplementation with n-6/n-3 Essential Fatty Acids in a 1.2 : 1.0 Ratio Attenuates Metabolic Dysfunction in MSG-Induced Obese Mice |
| title_fullStr | Maternal Diet Supplementation with n-6/n-3 Essential Fatty Acids in a 1.2 : 1.0 Ratio Attenuates Metabolic Dysfunction in MSG-Induced Obese Mice |
| title_full_unstemmed | Maternal Diet Supplementation with n-6/n-3 Essential Fatty Acids in a 1.2 : 1.0 Ratio Attenuates Metabolic Dysfunction in MSG-Induced Obese Mice |
| title_short | Maternal Diet Supplementation with n-6/n-3 Essential Fatty Acids in a 1.2 : 1.0 Ratio Attenuates Metabolic Dysfunction in MSG-Induced Obese Mice |
| title_sort | maternal diet supplementation with n 6 n 3 essential fatty acids in a 1 2 1 0 ratio attenuates metabolic dysfunction in msg induced obese mice |
| url | http://dx.doi.org/10.1155/2016/9242319 |
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