Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root–Propolis Extract on HEK293T Cells
<b>Background/Objectives:</b> The health benefits of honeybee products and herbs are well known, and their appropriate combination may enhance their biological efficacy. This study investigated the biological properties of a combined barberry root and propolis extract (PBE) in comparison...
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2024-12-01
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| author | Dana Marcinčáková Nikola Hudáková Michal Miłek Mária Kolesárová Małgorzata Dżugan Dasa Cizkova Jaroslav Legáth |
| author_facet | Dana Marcinčáková Nikola Hudáková Michal Miłek Mária Kolesárová Małgorzata Dżugan Dasa Cizkova Jaroslav Legáth |
| author_sort | Dana Marcinčáková |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> The health benefits of honeybee products and herbs are well known, and their appropriate combination may enhance their biological efficacy. This study investigated the biological properties of a combined barberry root and propolis extract (PBE) in comparison to a propolis extract (PE), a barberry root extract (BE), and pure berberine (BN). <b>Methods:</b> The antioxidant properties were evaluated using DPPH and FRAP methods and total phenolic contents (TPC) were assessed by the Folin–Ciocalteu method. HPTLC was used to quantify the BE in the tested samples. Their effect on HEK293T cells was monitored in real-time by using the xCELLigence system which recorded changes in the proliferative activity (PA). The metabolic activity (MA) was evaluated using an MTS test and cell migration was analyzed via a scratch assay. <b>Results:</b> The PE exhibited a higher TPC (198.67 mg/g) than the BE (119.3 mg/g). The PBE exhibited a comparable antioxidant effect to that of the PE. In the cell assays, the PE, the BE, and BN significantly reduced the proliferative activity at higher concentrations (<i>p</i> < 0.0001) while the PBE demonstrated a lower cytotoxicity and proved to be safer for the tested cells. The highest IC<sub>50</sub> value was determined for the PBE (130 µg/mL), suggesting that this combination has a reduced cytotoxicity. However, the scratch test did not confirm a significant supportive effect of the PBE on cell migration. <b>Conclusions:</b> Although the PBE did not show enhanced antioxidant properties, it may mitigate cytotoxicity and support proliferation at lower concentrations. This suggests that extraction of raw propolis with a previously prepared barberry extract results in a safer preparation, but its therapeutic potential requires further studies using biological models. |
| format | Article |
| id | doaj-art-bc36ccc6e2414bc7832f56604f0cf326 |
| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-bc36ccc6e2414bc7832f56604f0cf3262025-01-24T13:45:04ZengMDPI AGPharmaceuticals1424-82472024-12-011812710.3390/ph18010027Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root–Propolis Extract on HEK293T CellsDana Marcinčáková0Nikola Hudáková1Michal Miłek2Mária Kolesárová3Małgorzata Dżugan4Dasa Cizkova5Jaroslav Legáth6Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Kosice, Komenského 73, 041 81 Kosice, SlovakiaCentre of Experimental and Clinical Regenerative Medicine, Small Animal Clinic, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, SlovakiaDepartment of Chemistry and Food Toxicology, Institute of Food Technology and Nutrition, University of Rzeszow, Ćwiklińskiej 1a St., 35-601 Rzeszow, PolandDepartment of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Kosice, Komenského 73, 041 81 Kosice, SlovakiaDepartment of Chemistry and Food Toxicology, Institute of Food Technology and Nutrition, University of Rzeszow, Ćwiklińskiej 1a St., 35-601 Rzeszow, PolandCentre of Experimental and Clinical Regenerative Medicine, Small Animal Clinic, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, SlovakiaDepartment of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Kosice, Komenského 73, 041 81 Kosice, Slovakia<b>Background/Objectives:</b> The health benefits of honeybee products and herbs are well known, and their appropriate combination may enhance their biological efficacy. This study investigated the biological properties of a combined barberry root and propolis extract (PBE) in comparison to a propolis extract (PE), a barberry root extract (BE), and pure berberine (BN). <b>Methods:</b> The antioxidant properties were evaluated using DPPH and FRAP methods and total phenolic contents (TPC) were assessed by the Folin–Ciocalteu method. HPTLC was used to quantify the BE in the tested samples. Their effect on HEK293T cells was monitored in real-time by using the xCELLigence system which recorded changes in the proliferative activity (PA). The metabolic activity (MA) was evaluated using an MTS test and cell migration was analyzed via a scratch assay. <b>Results:</b> The PE exhibited a higher TPC (198.67 mg/g) than the BE (119.3 mg/g). The PBE exhibited a comparable antioxidant effect to that of the PE. In the cell assays, the PE, the BE, and BN significantly reduced the proliferative activity at higher concentrations (<i>p</i> < 0.0001) while the PBE demonstrated a lower cytotoxicity and proved to be safer for the tested cells. The highest IC<sub>50</sub> value was determined for the PBE (130 µg/mL), suggesting that this combination has a reduced cytotoxicity. However, the scratch test did not confirm a significant supportive effect of the PBE on cell migration. <b>Conclusions:</b> Although the PBE did not show enhanced antioxidant properties, it may mitigate cytotoxicity and support proliferation at lower concentrations. This suggests that extraction of raw propolis with a previously prepared barberry extract results in a safer preparation, but its therapeutic potential requires further studies using biological models.https://www.mdpi.com/1424-8247/18/1/27propolisbarberryberberineantioxidantHPTLCcell proliferation |
| spellingShingle | Dana Marcinčáková Nikola Hudáková Michal Miłek Mária Kolesárová Małgorzata Dżugan Dasa Cizkova Jaroslav Legáth Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root–Propolis Extract on HEK293T Cells Pharmaceuticals propolis barberry berberine antioxidant HPTLC cell proliferation |
| title | Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root–Propolis Extract on HEK293T Cells |
| title_full | Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root–Propolis Extract on HEK293T Cells |
| title_fullStr | Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root–Propolis Extract on HEK293T Cells |
| title_full_unstemmed | Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root–Propolis Extract on HEK293T Cells |
| title_short | Evaluation of the Antioxidant Properties and Biological Effects of a Novel Combined Barberry Root–Propolis Extract on HEK293T Cells |
| title_sort | evaluation of the antioxidant properties and biological effects of a novel combined barberry root propolis extract on hek293t cells |
| topic | propolis barberry berberine antioxidant HPTLC cell proliferation |
| url | https://www.mdpi.com/1424-8247/18/1/27 |
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