Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II
A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR® green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quali...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2000-07-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00291st01 |
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| _version_ | 1850151910585139200 |
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| author | C. Battaglia G. Salani C. Consolandi L. Rossi Bernardi G. De Bellis |
| author_facet | C. Battaglia G. Salani C. Consolandi L. Rossi Bernardi G. De Bellis |
| author_sort | C. Battaglia |
| collection | DOAJ |
| description | A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR® green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preliminary control, which may avoid further analytical steps that lead to the waste of precious biological samples, a fully reversible staining procedure is performed that produces an array ready for subsequent use. |
| format | Article |
| id | doaj-art-bc2bf0e67d864e30bcb09d4d8287e9f3 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2000-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-bc2bf0e67d864e30bcb09d4d8287e9f32025-08-20T02:26:06ZengTaylor & Francis GroupBioTechniques0736-62051940-98182000-07-01291788110.2144/00291st01Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green IIC. Battaglia0G. Salani1C. Consolandi2L. Rossi Bernardi3G. De Bellis41Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche Avanzate, Segrate, Italy1Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche Avanzate, Segrate, Italy1Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche Avanzate, Segrate, Italy1Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche Avanzate, Segrate, Italy1Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche Avanzate, Segrate, ItalyA simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR® green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preliminary control, which may avoid further analytical steps that lead to the waste of precious biological samples, a fully reversible staining procedure is performed that produces an array ready for subsequent use.https://www.future-science.com/doi/10.2144/00291st01 |
| spellingShingle | C. Battaglia G. Salani C. Consolandi L. Rossi Bernardi G. De Bellis Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II BioTechniques |
| title | Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II |
| title_full | Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II |
| title_fullStr | Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II |
| title_full_unstemmed | Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II |
| title_short | Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II |
| title_sort | analysis of dna microarrays by non destructive fluorescent staining using sybr r green ii |
| url | https://www.future-science.com/doi/10.2144/00291st01 |
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