Efficacy of Topical Mesenchymal Stem Cell Therapy in the Treatment of Experimental Dry Eye Syndrome Model
Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing eit...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2014/250230 |
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| author | Emrullah Beyazyıldız Ferda Alpaslan Pınarlı Özlem Beyazyıldız Emine Rümeysa Hekimoğlu Uğur Acar Muhammed Necati Demir Aynur Albayrak Figen Kaymaz Güngör Sobacı Tuncay Delibaşı |
| author_facet | Emrullah Beyazyıldız Ferda Alpaslan Pınarlı Özlem Beyazyıldız Emine Rümeysa Hekimoğlu Uğur Acar Muhammed Necati Demir Aynur Albayrak Figen Kaymaz Güngör Sobacı Tuncay Delibaşı |
| author_sort | Emrullah Beyazyıldız |
| collection | DOAJ |
| description | Purpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridine labeled MSCs (n=9) or phosphate buffer solution (n=7) were topically applied once daily for one week. Schirmer test, break-up time score, ocular surface evaluation tests, and corneal inflammatory index scoring tests were applied to all rats at baseline and after treatment. All rats were sacrificed after one week for histological and electron microscopic analysis. Results. Mean aqueous tear volume and tear film stability were significantly increased in rats treated with MSCs (P<0.05). Infiltration of bromodeoxyuridine labeled MSCs into the meibomian glands and conjunctival epithelium was observed in MSCs treated rats. Increased number of secretory granules and number of goblet cells were observed in MSCs treated rats. Conclusion. Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies. |
| format | Article |
| id | doaj-art-bc19cf4f78624fcdb5413de001139a30 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-bc19cf4f78624fcdb5413de001139a302025-02-03T06:42:25ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/250230250230Efficacy of Topical Mesenchymal Stem Cell Therapy in the Treatment of Experimental Dry Eye Syndrome ModelEmrullah Beyazyıldız0Ferda Alpaslan Pınarlı1Özlem Beyazyıldız2Emine Rümeysa Hekimoğlu3Uğur Acar4Muhammed Necati Demir5Aynur Albayrak6Figen Kaymaz7Güngör Sobacı8Tuncay Delibaşı9Department of Ophthalmology, Samsun Research and Training Hospital, Kıranköy Mevkii, 55100 Samsun, TurkeyCenter of Cell Research and Genetic Diagnosis, Dışkapı Yıldırım Beyazıt Research Hospital, Etlik, 06010 Ankara, TurkeyDepartment of Ophthalmology, Samsun Research and Training Hospital, Kıranköy Mevkii, 55100 Samsun, TurkeyDepartment of Histology and Embryology, Kastamonu Faculty of Medicine, Hacettepe University, 06230 Ankara, TurkeyDepartment of Ophthalmology, Kastamonu Faculty of Medicine, Hacettepe University, 06230 Ankara, TurkeyDepartment of Ophthalmology, Faculty of Medicine, Yıldırım Beyazıt University, Bilkent, 06800 Ankara, TurkeyDepartment of Pathology, Dışkapı Yıldırım Beyazıt Research and Training Hospital, Dışkapı, 06110 Ankara, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Hacettepe University, 06230 Ankara, TurkeyDepartment of Ophthalmology, Faculty of Medicine, Hacettepe University, 06230 Ankara, TurkeyDepartment of Endocrinology, Kastamonu Faculty of Medicine, Hacettepe University, 06230 Ankara, TurkeyPurpose. The current study was set out to address the therapeutic efficacy of topically applied mesenchymal stem cells (MSCs) on dry eye syndrome (DES) induced by benzalkonium chloride (BAC) in rats. Methods. Rats were divided into two groups just after establishment of DES. Eye drops containing either bromodeoxyuridine labeled MSCs (n=9) or phosphate buffer solution (n=7) were topically applied once daily for one week. Schirmer test, break-up time score, ocular surface evaluation tests, and corneal inflammatory index scoring tests were applied to all rats at baseline and after treatment. All rats were sacrificed after one week for histological and electron microscopic analysis. Results. Mean aqueous tear volume and tear film stability were significantly increased in rats treated with MSCs (P<0.05). Infiltration of bromodeoxyuridine labeled MSCs into the meibomian glands and conjunctival epithelium was observed in MSCs treated rats. Increased number of secretory granules and number of goblet cells were observed in MSCs treated rats. Conclusion. Topical application of MSCs could be a safe and effective method for the treatment of DES and could potentially be used for further clinical research studies.http://dx.doi.org/10.1155/2014/250230 |
| spellingShingle | Emrullah Beyazyıldız Ferda Alpaslan Pınarlı Özlem Beyazyıldız Emine Rümeysa Hekimoğlu Uğur Acar Muhammed Necati Demir Aynur Albayrak Figen Kaymaz Güngör Sobacı Tuncay Delibaşı Efficacy of Topical Mesenchymal Stem Cell Therapy in the Treatment of Experimental Dry Eye Syndrome Model Stem Cells International |
| title | Efficacy of Topical Mesenchymal Stem Cell Therapy in the Treatment of Experimental Dry Eye Syndrome Model |
| title_full | Efficacy of Topical Mesenchymal Stem Cell Therapy in the Treatment of Experimental Dry Eye Syndrome Model |
| title_fullStr | Efficacy of Topical Mesenchymal Stem Cell Therapy in the Treatment of Experimental Dry Eye Syndrome Model |
| title_full_unstemmed | Efficacy of Topical Mesenchymal Stem Cell Therapy in the Treatment of Experimental Dry Eye Syndrome Model |
| title_short | Efficacy of Topical Mesenchymal Stem Cell Therapy in the Treatment of Experimental Dry Eye Syndrome Model |
| title_sort | efficacy of topical mesenchymal stem cell therapy in the treatment of experimental dry eye syndrome model |
| url | http://dx.doi.org/10.1155/2014/250230 |
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