Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va
Leishmaniasis is a neglected tropical disease with no effective vaccines. Actin, microtubules and the actin-based molecular motor myosin Va were investigated for their involvement in Leishmania braziliensis macrophage interactions. Results showed a decrease in the association index when macrophages...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2012/275436 |
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| author | Elisama Azevedo Leandro Teixeira Oliveira Ana Karina Castro Lima Rodrigo Terra Patrícia Maria Lourenço Dutra Verônica P. Salerno |
| author_facet | Elisama Azevedo Leandro Teixeira Oliveira Ana Karina Castro Lima Rodrigo Terra Patrícia Maria Lourenço Dutra Verônica P. Salerno |
| author_sort | Elisama Azevedo |
| collection | DOAJ |
| description | Leishmaniasis is a neglected tropical disease with no effective vaccines. Actin, microtubules and the actin-based molecular motor myosin Va were investigated for their involvement in Leishmania braziliensis macrophage interactions. Results showed a decrease in the association index when macrophages were without F-actin or microtubules regardless of the activation state of the macrophage. In the absence of F-actin, the production of NO in non-activated cells increased, while in activated cells, the production of NO was reduced independent of parasites. The opposite effect of an increased NO production was observed in the absence of microtubules. In activated cells, the loss of cytoskeletal components inhibited the release of IL-10 during parasite interactions. The production of IL-10 also decreased in the absence of actin or microtubules in non-activated macrophages. Only the disruption of actin altered the production of TNF-α in activated macrophages. The expression of myosin Va tail resulted in an acute decrease in the association index between transfected macrophages and L. braziliensis promastigotes. These data reveal the importance of F-actin, microtubules, and myosin-Va suggesting that modulation of the cytoskeleton may be a mechanism used by L. braziliensis to overcome the natural responses of macrophages to establish infections. |
| format | Article |
| id | doaj-art-bc14216a8cf545049df85a35e2b28bb1 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-bc14216a8cf545049df85a35e2b28bb12025-02-03T05:59:12ZengWileyJournal of Parasitology Research2090-00232090-00312012-01-01201210.1155/2012/275436275436Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin VaElisama Azevedo0Leandro Teixeira Oliveira1Ana Karina Castro Lima2Rodrigo Terra3Patrícia Maria Lourenço Dutra4Verônica P. Salerno5Laboratório de Imunologia e Bioquímica de Protozoários, Departamento de Microbiologia, Imunologia e Parasitologia, FCM, UERJ, Avenida Professor Manuel de Abreu 444 5∘ andar. Vila Isabel, 20550-170 Rio de Janeiro, RJ, BrazilDepartamento Biociências, Escola de Educação Física e Desportos, Universidade Federal do Rio de Janeiro, 21941-599 Rio de Janerio, RJ, BrazilLaboratório de Imunologia e Bioquímica de Protozoários, Departamento de Microbiologia, Imunologia e Parasitologia, FCM, UERJ, Avenida Professor Manuel de Abreu 444 5∘ andar. Vila Isabel, 20550-170 Rio de Janeiro, RJ, BrazilLaboratório de Imunologia e Bioquímica de Protozoários, Departamento de Microbiologia, Imunologia e Parasitologia, FCM, UERJ, Avenida Professor Manuel de Abreu 444 5∘ andar. Vila Isabel, 20550-170 Rio de Janeiro, RJ, BrazilLaboratório de Imunologia e Bioquímica de Protozoários, Departamento de Microbiologia, Imunologia e Parasitologia, FCM, UERJ, Avenida Professor Manuel de Abreu 444 5∘ andar. Vila Isabel, 20550-170 Rio de Janeiro, RJ, BrazilDepartamento Biociências, Escola de Educação Física e Desportos, Universidade Federal do Rio de Janeiro, 21941-599 Rio de Janerio, RJ, BrazilLeishmaniasis is a neglected tropical disease with no effective vaccines. Actin, microtubules and the actin-based molecular motor myosin Va were investigated for their involvement in Leishmania braziliensis macrophage interactions. Results showed a decrease in the association index when macrophages were without F-actin or microtubules regardless of the activation state of the macrophage. In the absence of F-actin, the production of NO in non-activated cells increased, while in activated cells, the production of NO was reduced independent of parasites. The opposite effect of an increased NO production was observed in the absence of microtubules. In activated cells, the loss of cytoskeletal components inhibited the release of IL-10 during parasite interactions. The production of IL-10 also decreased in the absence of actin or microtubules in non-activated macrophages. Only the disruption of actin altered the production of TNF-α in activated macrophages. The expression of myosin Va tail resulted in an acute decrease in the association index between transfected macrophages and L. braziliensis promastigotes. These data reveal the importance of F-actin, microtubules, and myosin-Va suggesting that modulation of the cytoskeleton may be a mechanism used by L. braziliensis to overcome the natural responses of macrophages to establish infections.http://dx.doi.org/10.1155/2012/275436 |
| spellingShingle | Elisama Azevedo Leandro Teixeira Oliveira Ana Karina Castro Lima Rodrigo Terra Patrícia Maria Lourenço Dutra Verônica P. Salerno Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va Journal of Parasitology Research |
| title | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
| title_full | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
| title_fullStr | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
| title_full_unstemmed | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
| title_short | Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va |
| title_sort | interactions between leishmania braziliensis and macrophages are dependent on the cytoskeleton and myosin va |
| url | http://dx.doi.org/10.1155/2012/275436 |
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