In-vitro effectiveness of aqueous extract of organic compost against Helminthosporium turcicum (cryptogamic disease pathogen of maize)
Sustainable agriculture requires the application of biofertilizers and biopesticides in farming systems. The aim of the study was to analyze in-vitro the effect of aqueous extracts from organic compost on the development of cryptogamic disease in maize plants for yield improvement. The study was car...
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IJARIT Research Foundation
2024-12-01
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| Series: | International Journal of Agricultural Research, Innovation and Technology |
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| Online Access: | https://www.banglajol.info/index.php/IJARIT/article/view/79401/52049 |
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| author | Daouda Kutnjem Anne Ayo Ecclèsiaste Marien Ambombo Onguene Carole Christèle Egono Ndemé Ndongo Bekolo Tofel Haman Katamssadan Asseng Charles Carnot |
| author_facet | Daouda Kutnjem Anne Ayo Ecclèsiaste Marien Ambombo Onguene Carole Christèle Egono Ndemé Ndongo Bekolo Tofel Haman Katamssadan Asseng Charles Carnot |
| author_sort | Daouda Kutnjem |
| collection | DOAJ |
| description | Sustainable agriculture requires the application of biofertilizers and biopesticides in farming systems. The aim of the study was to analyze in-vitro the effect of aqueous extracts from organic compost on the development of cryptogamic disease in maize plants for yield improvement. The study was carried out in the laboratory using a pure strain of Helminthosporium turcicum previously isolated from infected maize leaves showing symptoms of the disease. Aqueous substrates of unsterilized and sterilized composts, with the concentrations 0.5, 1.0 and 2.0 mL were respectively mixed with liquid PDA at 19.5; 19 and 18 mL for a final volume of 20 ml, then aseptically poured into 90 mm diameter Petri dishes under the laminar flow hood. After solidification, 7 mm-diameter mycelial discs from young H. turcicum cultures were deposited on the agar pellets. 48 hours after incubation, mycelial growth was measured 4 times until the positive control Petri dish was invaded by mycelial filaments. The results showed that the mycelial growth of the H. turcicum strain in the presence of the sterilized aqueous compost extract was significantly reduced. Increasing the concentration significantly inhibited strain growth to the order of 95.89% at a concentration of 2 mL sterilized aqueous compost extract. Similarly, in the presence of the unsterilized aqueous compost extract, the mycelial growth of H. turcicum was also reduced. The Increasing concentration inhibited the pathogen growth by up to 76.92%, at the concentration of 2 mL. These results showed that the aqueous extract of organic compost contains compounds that could be responsible for producing suppressive substances accountable for the mechanisms inhibiting the mycelial growth of H. turcicum. |
| format | Article |
| id | doaj-art-bc078a16d5d84019957a98a3b229e081 |
| institution | Kabale University |
| issn | 2224-0616 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | IJARIT Research Foundation |
| record_format | Article |
| series | International Journal of Agricultural Research, Innovation and Technology |
| spelling | doaj-art-bc078a16d5d84019957a98a3b229e0812025-02-02T16:19:58ZengIJARIT Research FoundationInternational Journal of Agricultural Research, Innovation and Technology2224-06162024-12-011426273https://doi.org/10.3329/ijarit.v14i2.79401In-vitro effectiveness of aqueous extract of organic compost against Helminthosporium turcicum (cryptogamic disease pathogen of maize)Daouda Kutnjem0https://orcid.org/0000-0002-5501-1837Anne Ayo1https://orcid.org/0000-0001-7786-4235Ecclèsiaste Marien Ambombo Onguene2https://orcid.org/0000-0003-4448-0467Carole Christèle Egono Ndemé3Ndongo Bekolo4https://orcid.org/0000-0002-2479-8382Tofel Haman Katamssadan5https://orcid.org/0000-0003-1791-3888Asseng Charles Carnot6Department of Phytosanitary Protection, ISABEE, University of Bertoua, PO Box 60 Belabo, CameroonLaboratory of Biotechnology and Environment, Department of Plant Biology, Faculty of Science, University of Yaoundé I, PO Box 812, Yaoundé, CameroonDepartment of Plant Production, ISABEE, University of Bertoua, PO Box 60 Belabo, CameroonDepartment of Environmental Sciences, ISABEE, University of Bertoua, PO Box 60 Belabo, CameroonLaboratory of Phytopathology, Biotechnology and Environment, Department of Plant Biology, University of Yaoundé I, PO Box 812, Yaoundé, CameroonDepartment of Phytosanitary Protection, ISABEE, University of Bertoua, PO Box 60 Belabo, CameroonDepartment of Plant Production, ISABEE, University of Bertoua, PO Box 60 Belabo, CameroonSustainable agriculture requires the application of biofertilizers and biopesticides in farming systems. The aim of the study was to analyze in-vitro the effect of aqueous extracts from organic compost on the development of cryptogamic disease in maize plants for yield improvement. The study was carried out in the laboratory using a pure strain of Helminthosporium turcicum previously isolated from infected maize leaves showing symptoms of the disease. Aqueous substrates of unsterilized and sterilized composts, with the concentrations 0.5, 1.0 and 2.0 mL were respectively mixed with liquid PDA at 19.5; 19 and 18 mL for a final volume of 20 ml, then aseptically poured into 90 mm diameter Petri dishes under the laminar flow hood. After solidification, 7 mm-diameter mycelial discs from young H. turcicum cultures were deposited on the agar pellets. 48 hours after incubation, mycelial growth was measured 4 times until the positive control Petri dish was invaded by mycelial filaments. The results showed that the mycelial growth of the H. turcicum strain in the presence of the sterilized aqueous compost extract was significantly reduced. Increasing the concentration significantly inhibited strain growth to the order of 95.89% at a concentration of 2 mL sterilized aqueous compost extract. Similarly, in the presence of the unsterilized aqueous compost extract, the mycelial growth of H. turcicum was also reduced. The Increasing concentration inhibited the pathogen growth by up to 76.92%, at the concentration of 2 mL. These results showed that the aqueous extract of organic compost contains compounds that could be responsible for producing suppressive substances accountable for the mechanisms inhibiting the mycelial growth of H. turcicum.https://www.banglajol.info/index.php/IJARIT/article/view/79401/52049aqueous compost extractmycelial growthhelminthosporium turcicumpda middlemaize |
| spellingShingle | Daouda Kutnjem Anne Ayo Ecclèsiaste Marien Ambombo Onguene Carole Christèle Egono Ndemé Ndongo Bekolo Tofel Haman Katamssadan Asseng Charles Carnot In-vitro effectiveness of aqueous extract of organic compost against Helminthosporium turcicum (cryptogamic disease pathogen of maize) International Journal of Agricultural Research, Innovation and Technology aqueous compost extract mycelial growth helminthosporium turcicum pda middle maize |
| title | In-vitro effectiveness of aqueous extract of organic compost against Helminthosporium turcicum (cryptogamic disease pathogen of maize) |
| title_full | In-vitro effectiveness of aqueous extract of organic compost against Helminthosporium turcicum (cryptogamic disease pathogen of maize) |
| title_fullStr | In-vitro effectiveness of aqueous extract of organic compost against Helminthosporium turcicum (cryptogamic disease pathogen of maize) |
| title_full_unstemmed | In-vitro effectiveness of aqueous extract of organic compost against Helminthosporium turcicum (cryptogamic disease pathogen of maize) |
| title_short | In-vitro effectiveness of aqueous extract of organic compost against Helminthosporium turcicum (cryptogamic disease pathogen of maize) |
| title_sort | in vitro effectiveness of aqueous extract of organic compost against helminthosporium turcicum cryptogamic disease pathogen of maize |
| topic | aqueous compost extract mycelial growth helminthosporium turcicum pda middle maize |
| url | https://www.banglajol.info/index.php/IJARIT/article/view/79401/52049 |
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