Synthesis of Functional Polyester Based on Polylactic Acid and Its Effect on PC12 Cells after Coupling with Small Peptides
Polyesters containing functional groups are a suitable candidate matrix for cell culture in tissue engineering. Three types of semicrystalline copolymer poly(L-lactide-co-β-malic acid) [P(LA-co-BMD)] with pendent carboxyl groups were synthesized in this study. The functional monomer 3(S)-[(benzyloxy...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2016/9829757 |
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| _version_ | 1832554311229374464 |
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| author | Na Qiang Shuo Tang Xiang Liao Hao Liang Fang Xie Ji-xiang Zhu |
| author_facet | Na Qiang Shuo Tang Xiang Liao Hao Liang Fang Xie Ji-xiang Zhu |
| author_sort | Na Qiang |
| collection | DOAJ |
| description | Polyesters containing functional groups are a suitable candidate matrix for cell culture in tissue engineering. Three types of semicrystalline copolymer poly(L-lactide-co-β-malic acid) [P(LA-co-BMD)] with pendent carboxyl groups were synthesized in this study. The functional monomer 3(S)-[(benzyloxycarbonyl)methyl]-1,4-dioxane-2,5-dione (BMD) was synthesized using L-aspartic acid. The copolymer P(LA-co-BMD) was then synthesized through ring-opening copolymerization of L-LA and BMD, with dodecanol as initiator and stannous octoate as catalyst. Copolymer structure was characterized by 1H nuclear magnetic resonance (1H NMR), gel permeation chromatography (GPC), and differential scanning calorimetry (DSC) analyses. Results of 1H NMR and GPC analyses showed that the copolymers were synthesized successfully. DSC curves showed that the crystal melting peak and enthalpy decreased with increased BMD. The crystallinity of the copolymer was destroyed by the presence of the functional monomer. After deprotection, carboxyl groups were coupled with the isoleucine-lysine-valine-alanine-valine peptide through N-hydroxysuccinimide/dicyclohexylcarbodiimide method. The small peptide was beneficial to the axon growth of PC12 cells. |
| format | Article |
| id | doaj-art-bc0115fa58464a11a0fee3d459a1a2d3 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-bc0115fa58464a11a0fee3d459a1a2d32025-02-03T05:51:54ZengWileyInternational Journal of Polymer Science1687-94221687-94302016-01-01201610.1155/2016/98297579829757Synthesis of Functional Polyester Based on Polylactic Acid and Its Effect on PC12 Cells after Coupling with Small PeptidesNa Qiang0Shuo Tang1Xiang Liao2Hao Liang3Fang Xie4Ji-xiang Zhu5Department of Chemical Engineering, Huizhou University, Huizhou 516007, ChinaDepartment of Pain Medicine, Nanshan Hospital, Shenzhen 51700, ChinaDepartment of Pain Medicine, Nanshan Hospital, Shenzhen 51700, ChinaDepartment of Chemical Engineering, Huizhou University, Huizhou 516007, ChinaDepartment of Chemical Engineering, Huizhou University, Huizhou 516007, ChinaDepartment of Biomedical Engineering, Guangzhou Medical University, Guangzhou 510182, ChinaPolyesters containing functional groups are a suitable candidate matrix for cell culture in tissue engineering. Three types of semicrystalline copolymer poly(L-lactide-co-β-malic acid) [P(LA-co-BMD)] with pendent carboxyl groups were synthesized in this study. The functional monomer 3(S)-[(benzyloxycarbonyl)methyl]-1,4-dioxane-2,5-dione (BMD) was synthesized using L-aspartic acid. The copolymer P(LA-co-BMD) was then synthesized through ring-opening copolymerization of L-LA and BMD, with dodecanol as initiator and stannous octoate as catalyst. Copolymer structure was characterized by 1H nuclear magnetic resonance (1H NMR), gel permeation chromatography (GPC), and differential scanning calorimetry (DSC) analyses. Results of 1H NMR and GPC analyses showed that the copolymers were synthesized successfully. DSC curves showed that the crystal melting peak and enthalpy decreased with increased BMD. The crystallinity of the copolymer was destroyed by the presence of the functional monomer. After deprotection, carboxyl groups were coupled with the isoleucine-lysine-valine-alanine-valine peptide through N-hydroxysuccinimide/dicyclohexylcarbodiimide method. The small peptide was beneficial to the axon growth of PC12 cells.http://dx.doi.org/10.1155/2016/9829757 |
| spellingShingle | Na Qiang Shuo Tang Xiang Liao Hao Liang Fang Xie Ji-xiang Zhu Synthesis of Functional Polyester Based on Polylactic Acid and Its Effect on PC12 Cells after Coupling with Small Peptides International Journal of Polymer Science |
| title | Synthesis of Functional Polyester Based on Polylactic Acid and Its Effect on PC12 Cells after Coupling with Small Peptides |
| title_full | Synthesis of Functional Polyester Based on Polylactic Acid and Its Effect on PC12 Cells after Coupling with Small Peptides |
| title_fullStr | Synthesis of Functional Polyester Based on Polylactic Acid and Its Effect on PC12 Cells after Coupling with Small Peptides |
| title_full_unstemmed | Synthesis of Functional Polyester Based on Polylactic Acid and Its Effect on PC12 Cells after Coupling with Small Peptides |
| title_short | Synthesis of Functional Polyester Based on Polylactic Acid and Its Effect on PC12 Cells after Coupling with Small Peptides |
| title_sort | synthesis of functional polyester based on polylactic acid and its effect on pc12 cells after coupling with small peptides |
| url | http://dx.doi.org/10.1155/2016/9829757 |
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