Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles
Nanoparticles influence on genome is investigated worldwide. The appearance of somaclonal variation is a cause of great concern for any micropropagation system. Somaclonal variation describes the tissue-culture-induced phenotypic and genotypic variations. This paper shows the results of somaclonal v...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/1676874 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832552385998749696 |
|---|---|
| author | Inese Kokina Ilona Mickeviča Marija Jermaļonoka Linda Bankovska Vjačeslavs Gerbreders Andrejs Ogurcovs Inese Jahundoviča |
| author_facet | Inese Kokina Ilona Mickeviča Marija Jermaļonoka Linda Bankovska Vjačeslavs Gerbreders Andrejs Ogurcovs Inese Jahundoviča |
| author_sort | Inese Kokina |
| collection | DOAJ |
| description | Nanoparticles influence on genome is investigated worldwide. The appearance of somaclonal variation is a cause of great concern for any micropropagation system. Somaclonal variation describes the tissue-culture-induced phenotypic and genotypic variations. This paper shows the results of somaclonal variation in two resistance genes pectin methylesterase and Mlo-like protein in all tissue culture development stages, as donor plant, calluses, and regenerants of Linum usitatissimum induced by gold and silver nanoparticles. In this paper, it was essential to obtain DNA material from all tissue culture development stages from one donor plant to record changes in each nucleotide sequence. Gene region specific primers were developed for resistance genes such as Mlo and Pme3 to define the genetic variability in tissue culture of L. usitatissimum. In recent years, utilization of gold and silver nanoparticles in tissue culture is increased and the mechanisms of changes in genome induced by nanoparticles still remain unclear. Obtained data show the somaclonal variation increase in calluses obtained from one donor plant and grown on medium supplemented by gold nanoparticles (Mlo 14.68±0.98; Pme3 2.07±0.87) or silver nanoparticles (Mlo 12.01±0.43; Pme3 10.04±0.46) and decrease in regenerants. Morphological parameters of calluses showed a number of differences between each investigated culture group. |
| format | Article |
| id | doaj-art-bbfb1abdfe1e4d19be347d8508738784 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-bbfb1abdfe1e4d19be347d85087387842025-02-03T05:58:47ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/16768741676874Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by NanoparticlesInese Kokina0Ilona Mickeviča1Marija Jermaļonoka2Linda Bankovska3Vjačeslavs Gerbreders4Andrejs Ogurcovs5Inese Jahundoviča6Department of Biotechnology, Daugavpils University, Institute of Life Sciences and Technology, Laboratory of Genomics and Biotechnology, Parades Street 1A, Daugavpils LV-5401, LatviaDepartment of Biotechnology, Daugavpils University, Institute of Life Sciences and Technology, Laboratory of Genomics and Biotechnology, Parades Street 1A, Daugavpils LV-5401, LatviaDepartment of Biotechnology, Daugavpils University, Institute of Life Sciences and Technology, Laboratory of Genomics and Biotechnology, Parades Street 1A, Daugavpils LV-5401, LatviaDepartment of Biotechnology, Daugavpils University, Institute of Life Sciences and Technology, Laboratory of Genomics and Biotechnology, Parades Street 1A, Daugavpils LV-5401, LatviaDepartment of Technology, Daugavpils University, Institute of Life Sciences and Technology, G. Liberts’ Center of Innovative Microscopy, Parades street 1A, Daugavpils LV-5401, LatviaDepartment of Technology, Daugavpils University, Institute of Life Sciences and Technology, G. Liberts’ Center of Innovative Microscopy, Parades street 1A, Daugavpils LV-5401, LatviaDepartment of Biotechnology, Daugavpils University, Institute of Life Sciences and Technology, Laboratory of Genomics and Biotechnology, Parades Street 1A, Daugavpils LV-5401, LatviaNanoparticles influence on genome is investigated worldwide. The appearance of somaclonal variation is a cause of great concern for any micropropagation system. Somaclonal variation describes the tissue-culture-induced phenotypic and genotypic variations. This paper shows the results of somaclonal variation in two resistance genes pectin methylesterase and Mlo-like protein in all tissue culture development stages, as donor plant, calluses, and regenerants of Linum usitatissimum induced by gold and silver nanoparticles. In this paper, it was essential to obtain DNA material from all tissue culture development stages from one donor plant to record changes in each nucleotide sequence. Gene region specific primers were developed for resistance genes such as Mlo and Pme3 to define the genetic variability in tissue culture of L. usitatissimum. In recent years, utilization of gold and silver nanoparticles in tissue culture is increased and the mechanisms of changes in genome induced by nanoparticles still remain unclear. Obtained data show the somaclonal variation increase in calluses obtained from one donor plant and grown on medium supplemented by gold nanoparticles (Mlo 14.68±0.98; Pme3 2.07±0.87) or silver nanoparticles (Mlo 12.01±0.43; Pme3 10.04±0.46) and decrease in regenerants. Morphological parameters of calluses showed a number of differences between each investigated culture group.http://dx.doi.org/10.1155/2017/1676874 |
| spellingShingle | Inese Kokina Ilona Mickeviča Marija Jermaļonoka Linda Bankovska Vjačeslavs Gerbreders Andrejs Ogurcovs Inese Jahundoviča Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles International Journal of Genomics |
| title | Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles |
| title_full | Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles |
| title_fullStr | Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles |
| title_full_unstemmed | Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles |
| title_short | Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles |
| title_sort | case study of somaclonal variation in resistance genes mlo and pme3 in flaxseed linum usitatissimum l induced by nanoparticles |
| url | http://dx.doi.org/10.1155/2017/1676874 |
| work_keys_str_mv | AT inesekokina casestudyofsomaclonalvariationinresistancegenesmloandpme3inflaxseedlinumusitatissimumlinducedbynanoparticles AT ilonamickevica casestudyofsomaclonalvariationinresistancegenesmloandpme3inflaxseedlinumusitatissimumlinducedbynanoparticles AT marijajermalonoka casestudyofsomaclonalvariationinresistancegenesmloandpme3inflaxseedlinumusitatissimumlinducedbynanoparticles AT lindabankovska casestudyofsomaclonalvariationinresistancegenesmloandpme3inflaxseedlinumusitatissimumlinducedbynanoparticles AT vjaceslavsgerbreders casestudyofsomaclonalvariationinresistancegenesmloandpme3inflaxseedlinumusitatissimumlinducedbynanoparticles AT andrejsogurcovs casestudyofsomaclonalvariationinresistancegenesmloandpme3inflaxseedlinumusitatissimumlinducedbynanoparticles AT inesejahundovica casestudyofsomaclonalvariationinresistancegenesmloandpme3inflaxseedlinumusitatissimumlinducedbynanoparticles |