A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries
Background. gcrR gene acts as a negative regulator related to sucrose-dependent adherence in S. mutans. It is constructive to test the potential capacity of mutans with gcrR gene deficient in bacteria replacement therapy. Methods. In this study, we constructed the mutant by homologous recombination....
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/460202 |
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| author | Wenting Pan Tiantian Mao Qing-an Xu Jin Shao Chang Liu Mingwen Fan |
| author_facet | Wenting Pan Tiantian Mao Qing-an Xu Jin Shao Chang Liu Mingwen Fan |
| author_sort | Wenting Pan |
| collection | DOAJ |
| description | Background. gcrR gene acts as a negative regulator related to sucrose-dependent adherence in S. mutans. It is constructive to test the potential capacity of mutans with gcrR gene deficient in bacteria replacement therapy. Methods. In this study, we constructed the mutant by homologous recombination. The morphological characteristics of biofilms were analyzed by confocal laser scanning microscopy. S. mutans UA159 and the mutant MS-gcrR-def were inoculated, respectively, or together for competitive testing in vitro and in rat model. Results. Adhesion assay showed that the adhesion ability of the mutant increased relative to the wild type, especially in the early stage. MS-gcrR-def out-competed S. mutans UA159 in vitro biofilm, and correspondingly coinfection displayed significantly fewer caries in vivo. The former possessed both a lower level of acid production and a stronger colonization potential than S. mutans UA159. Conclusion. These findings demonstrate that MS-gcrR-def appears to be a good candidate for replacement therapy. |
| format | Article |
| id | doaj-art-bbfa1261e9e44a67be08c4309d338e84 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-bbfa1261e9e44a67be08c4309d338e842025-02-03T01:26:18ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/460202460202A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental CariesWenting Pan0Tiantian Mao1Qing-an Xu2Jin Shao3Chang Liu4Mingwen Fan5The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, ChinaDepartment of Stomatology, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430060, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, ChinaBackground. gcrR gene acts as a negative regulator related to sucrose-dependent adherence in S. mutans. It is constructive to test the potential capacity of mutans with gcrR gene deficient in bacteria replacement therapy. Methods. In this study, we constructed the mutant by homologous recombination. The morphological characteristics of biofilms were analyzed by confocal laser scanning microscopy. S. mutans UA159 and the mutant MS-gcrR-def were inoculated, respectively, or together for competitive testing in vitro and in rat model. Results. Adhesion assay showed that the adhesion ability of the mutant increased relative to the wild type, especially in the early stage. MS-gcrR-def out-competed S. mutans UA159 in vitro biofilm, and correspondingly coinfection displayed significantly fewer caries in vivo. The former possessed both a lower level of acid production and a stronger colonization potential than S. mutans UA159. Conclusion. These findings demonstrate that MS-gcrR-def appears to be a good candidate for replacement therapy.http://dx.doi.org/10.1155/2013/460202 |
| spellingShingle | Wenting Pan Tiantian Mao Qing-an Xu Jin Shao Chang Liu Mingwen Fan A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries The Scientific World Journal |
| title | A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries |
| title_full | A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries |
| title_fullStr | A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries |
| title_full_unstemmed | A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries |
| title_short | A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries |
| title_sort | new gcrr deficient streptococcus mutans mutant for replacement therapy of dental caries |
| url | http://dx.doi.org/10.1155/2013/460202 |
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