Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida
LexA is a well-known transcriptional repressor of DNA repair genes induced by DNA damage in Escherichia coli and other bacterial species. Recently, this paradigm—that LexA solely regulates the SOS response—has been challenged as studies reveal its involvement in various biological functions linked t...
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Elsevier
2025-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517425000136 |
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| author | Rongchao He Yanfei Zuo Qiu Li Qingpi Yan Lixing Huang |
| author_facet | Rongchao He Yanfei Zuo Qiu Li Qingpi Yan Lixing Huang |
| author_sort | Rongchao He |
| collection | DOAJ |
| description | LexA is a well-known transcriptional repressor of DNA repair genes induced by DNA damage in Escherichia coli and other bacterial species. Recently, this paradigm—that LexA solely regulates the SOS response—has been challenged as studies reveal its involvement in various biological functions linked to virulence. Pseudomonas plecoglossicida, a major pathogen in mariculture, causes substantial economic losses annually in China. Our previous research suggested that LexA might collaboratively regulate virulence gene expression with HtpG during infection. This study aims to elucidate the molecular mechanism by which LexA controls virulence gene expression. We employed an array of methods including molecular dynamics simulations, molecular docking, ChIP-seq, RNA-seq, mass spectrometry, gene mutagenesis, LacZ reporter assays, electrophoretic mobility shift assays, co-immunoprecipitation, and in vitro LexA degradation experiments. Our findings identified 36 downstream virulence genes regulated by LexA, define three critical LexA binding motifs, and provide an in-depth analysis of LexA's recognition and binding to promoters, thereby regulating virulence gene expression. Additionally, we confirm the cooperative regulatory roles of HtpG, RecA, and LexA in virulence gene modulation. This is the first report of an endogenous accessory factor aiding in the binding of LexA to DNA. This study enhances our understanding of LexA's role in virulence regulation and offers a valuable theoretical and practical foundation for disease prevention and control. |
| format | Article |
| id | doaj-art-bbca060b69cc473eb97d3690de30a18e |
| institution | Kabale University |
| issn | 2666-5174 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-bbca060b69cc473eb97d3690de30a18e2025-02-03T04:16:58ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-018100351Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicidaRongchao He0Yanfei Zuo1Qiu Li2Qingpi Yan3Lixing Huang4Fisheries College, Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Jimei University, Xiamen, Fujian, PR ChinaFisheries College, Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Jimei University, Xiamen, Fujian, PR ChinaFisheries College, Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Jimei University, Xiamen, Fujian, PR ChinaFisheries College, Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Jimei University, Xiamen, Fujian, PR China; State Key Laboratory of Mariculture Breeding, Fisheries college of Jimei university, Xiamen, Fujian, PR China; Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, Fujian 361021, PR China; Correspondence authors at: Fisheries College, Jimei University, Yindou Street 43, Xiamen, Fujian 361021, PR China.Fisheries College, Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Jimei University, Xiamen, Fujian, PR China; State Key Laboratory of Mariculture Breeding, Fisheries college of Jimei university, Xiamen, Fujian, PR China; Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, Fujian 361021, PR China; Correspondence authors at: Fisheries College, Jimei University, Yindou Street 43, Xiamen, Fujian 361021, PR China.LexA is a well-known transcriptional repressor of DNA repair genes induced by DNA damage in Escherichia coli and other bacterial species. Recently, this paradigm—that LexA solely regulates the SOS response—has been challenged as studies reveal its involvement in various biological functions linked to virulence. Pseudomonas plecoglossicida, a major pathogen in mariculture, causes substantial economic losses annually in China. Our previous research suggested that LexA might collaboratively regulate virulence gene expression with HtpG during infection. This study aims to elucidate the molecular mechanism by which LexA controls virulence gene expression. We employed an array of methods including molecular dynamics simulations, molecular docking, ChIP-seq, RNA-seq, mass spectrometry, gene mutagenesis, LacZ reporter assays, electrophoretic mobility shift assays, co-immunoprecipitation, and in vitro LexA degradation experiments. Our findings identified 36 downstream virulence genes regulated by LexA, define three critical LexA binding motifs, and provide an in-depth analysis of LexA's recognition and binding to promoters, thereby regulating virulence gene expression. Additionally, we confirm the cooperative regulatory roles of HtpG, RecA, and LexA in virulence gene modulation. This is the first report of an endogenous accessory factor aiding in the binding of LexA to DNA. This study enhances our understanding of LexA's role in virulence regulation and offers a valuable theoretical and practical foundation for disease prevention and control.http://www.sciencedirect.com/science/article/pii/S2666517425000136AdhesionBiofilm formationLexAPseudomonas plecoglossicidaVirulence regulation |
| spellingShingle | Rongchao He Yanfei Zuo Qiu Li Qingpi Yan Lixing Huang Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida Current Research in Microbial Sciences Adhesion Biofilm formation LexA Pseudomonas plecoglossicida Virulence regulation |
| title | Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida |
| title_full | Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida |
| title_fullStr | Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida |
| title_full_unstemmed | Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida |
| title_short | Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida |
| title_sort | cooperative mechanisms of lexa and htpg in the regulation of virulence gene expression in pseudomonas plecoglossicida |
| topic | Adhesion Biofilm formation LexA Pseudomonas plecoglossicida Virulence regulation |
| url | http://www.sciencedirect.com/science/article/pii/S2666517425000136 |
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