Topical application of the ethanol extract and the hydroethanolic fraction of ripe Solanum lycocarpum A. St. Hil. fruit improves the healing of excisional wounds
ABSTRACT Purpose: To evaluate the effect of the topical application of the ethanol extract (EESL) and the hydroethanolic fraction (HFSL) of ripe Solanum lycocarpum fruit on the healing of experimentally-induced wounds in mice. Methods: The EESL and HFSL obtained from ripe fruit of the species S. l...
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| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2025-01-01
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| Series: | Acta Cirúrgica Brasileira |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100206&lng=en&tlng=en |
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| author | Elisângela Elduina Ferreira Renan Diniz Ferreira Milena Santos de Almeida Renan Araújo Costa Izabela Caputo Assis Silva Melissa Grazielle Morais Karen Helaine Mendes Bertolin Paula Vieira Teixeira Vidigal Raquel Alves Costa Luciana Alves Rodrigues dos Santos Lima Flávia Carmo Horta Pinto |
| author_facet | Elisângela Elduina Ferreira Renan Diniz Ferreira Milena Santos de Almeida Renan Araújo Costa Izabela Caputo Assis Silva Melissa Grazielle Morais Karen Helaine Mendes Bertolin Paula Vieira Teixeira Vidigal Raquel Alves Costa Luciana Alves Rodrigues dos Santos Lima Flávia Carmo Horta Pinto |
| author_sort | Elisângela Elduina Ferreira |
| collection | DOAJ |
| description | ABSTRACT Purpose: To evaluate the effect of the topical application of the ethanol extract (EESL) and the hydroethanolic fraction (HFSL) of ripe Solanum lycocarpum fruit on the healing of experimentally-induced wounds in mice. Methods: The EESL and HFSL obtained from ripe fruit of the species S. lycocarpum were obtained by percolation with ethanol. They were tested in the healing of excisional wounds in mice, which were induced in anesthetized animals using a 7-mm dermatological punch. They were divided according to the treatment period and group, n = 6, and received topical application of the EESL or HFSL daily or saline solution for one, five, seven, or 21 days. At the end of this period, the animals were euthanized, and the lesions were subjected to histopathological processing and analysis to evaluate macroscopic area of the wound and microscopic analysis by morphometry of the number of leukocytes, mast cells, fibroblasts, re-epithelialization, and matrix deposition. Results: The application of the EESL and HFSL reduced the number of leukocytes after one, five, and seven days of treatment. EESL improved re-epithelialization and tissue proliferation in wound healing from day 7. Conclusion: The study demonstrated that daily administration of EESL and HFSL exhibit wound healing activity, with this effect being more pronounced by EESL. To our knowledge, this is the first report of the anti-inflammatory and healing activity of this species through topical application. |
| format | Article |
| id | doaj-art-bbace7f8053a46c396bd351782559db8 |
| institution | Kabale University |
| issn | 1678-2674 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj-art-bbace7f8053a46c396bd351782559db82025-01-21T07:45:43ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742025-01-014010.1590/acb400825Topical application of the ethanol extract and the hydroethanolic fraction of ripe Solanum lycocarpum A. St. Hil. fruit improves the healing of excisional woundsElisângela Elduina Ferreirahttps://orcid.org/0000-0003-1714-1275Renan Diniz Ferreirahttps://orcid.org/0000-0001-7160-7852Milena Santos de Almeidahttps://orcid.org/0009-0000-4890-9174Renan Araújo Costahttps://orcid.org/0000-0002-6599-4961Izabela Caputo Assis Silvahttps://orcid.org/0000-0001-5100-0690Melissa Grazielle Moraishttps://orcid.org/0000-0002-5825-9897Karen Helaine Mendes Bertolinhttps://orcid.org/0009-0003-8803-9742Paula Vieira Teixeira Vidigalhttps://orcid.org/0000-0002-7003-5159Raquel Alves Costahttps://orcid.org/0000-0003-2111-0527Luciana Alves Rodrigues dos Santos Limahttps://orcid.org/0000-0002-4130-0304Flávia Carmo Horta Pintohttps://orcid.org/0000-0003-3070-3930ABSTRACT Purpose: To evaluate the effect of the topical application of the ethanol extract (EESL) and the hydroethanolic fraction (HFSL) of ripe Solanum lycocarpum fruit on the healing of experimentally-induced wounds in mice. Methods: The EESL and HFSL obtained from ripe fruit of the species S. lycocarpum were obtained by percolation with ethanol. They were tested in the healing of excisional wounds in mice, which were induced in anesthetized animals using a 7-mm dermatological punch. They were divided according to the treatment period and group, n = 6, and received topical application of the EESL or HFSL daily or saline solution for one, five, seven, or 21 days. At the end of this period, the animals were euthanized, and the lesions were subjected to histopathological processing and analysis to evaluate macroscopic area of the wound and microscopic analysis by morphometry of the number of leukocytes, mast cells, fibroblasts, re-epithelialization, and matrix deposition. Results: The application of the EESL and HFSL reduced the number of leukocytes after one, five, and seven days of treatment. EESL improved re-epithelialization and tissue proliferation in wound healing from day 7. Conclusion: The study demonstrated that daily administration of EESL and HFSL exhibit wound healing activity, with this effect being more pronounced by EESL. To our knowledge, this is the first report of the anti-inflammatory and healing activity of this species through topical application.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100206&lng=en&tlng=enWound HealingSkinSolanumAnti-Inflammatory Agents |
| spellingShingle | Elisângela Elduina Ferreira Renan Diniz Ferreira Milena Santos de Almeida Renan Araújo Costa Izabela Caputo Assis Silva Melissa Grazielle Morais Karen Helaine Mendes Bertolin Paula Vieira Teixeira Vidigal Raquel Alves Costa Luciana Alves Rodrigues dos Santos Lima Flávia Carmo Horta Pinto Topical application of the ethanol extract and the hydroethanolic fraction of ripe Solanum lycocarpum A. St. Hil. fruit improves the healing of excisional wounds Acta Cirúrgica Brasileira Wound Healing Skin Solanum Anti-Inflammatory Agents |
| title | Topical application of the ethanol extract and the hydroethanolic fraction of ripe Solanum lycocarpum A. St. Hil. fruit improves the healing of excisional wounds |
| title_full | Topical application of the ethanol extract and the hydroethanolic fraction of ripe Solanum lycocarpum A. St. Hil. fruit improves the healing of excisional wounds |
| title_fullStr | Topical application of the ethanol extract and the hydroethanolic fraction of ripe Solanum lycocarpum A. St. Hil. fruit improves the healing of excisional wounds |
| title_full_unstemmed | Topical application of the ethanol extract and the hydroethanolic fraction of ripe Solanum lycocarpum A. St. Hil. fruit improves the healing of excisional wounds |
| title_short | Topical application of the ethanol extract and the hydroethanolic fraction of ripe Solanum lycocarpum A. St. Hil. fruit improves the healing of excisional wounds |
| title_sort | topical application of the ethanol extract and the hydroethanolic fraction of ripe solanum lycocarpum a st hil fruit improves the healing of excisional wounds |
| topic | Wound Healing Skin Solanum Anti-Inflammatory Agents |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100206&lng=en&tlng=en |
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