Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity
In this study, male Sprague–Dawley (SD) rats were exposed to bisphenol S (BPS) at environmentally relevant concentrations to investigate its reproductive toxicity and evaluate its effects on the gut-blood-testicular axis. After 28 days of exposure to BPS (0.05 and 20 mg/kg), the results showed a red...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324017226 |
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| author | Biran Zhu Zequan Zhang Ying Xie Min Huang Yu Chen Yong Yang Xiongjie Shi Jian Han Lihua Yang Min Zhao |
| author_facet | Biran Zhu Zequan Zhang Ying Xie Min Huang Yu Chen Yong Yang Xiongjie Shi Jian Han Lihua Yang Min Zhao |
| author_sort | Biran Zhu |
| collection | DOAJ |
| description | In this study, male Sprague–Dawley (SD) rats were exposed to bisphenol S (BPS) at environmentally relevant concentrations to investigate its reproductive toxicity and evaluate its effects on the gut-blood-testicular axis. After 28 days of exposure to BPS (0.05 and 20 mg/kg), the results showed a reduction in weight gain and the induction of reproductive toxicity in male rats, including decreased sperm parameters, lower sperm viability, and increased abnormal sperm density and mortality. These observations were made by counting with a hemocytometer under the optical microscope. 16S rRNA and untargeted metabolomic elucidated potential impacts on the gut-blood-testicular axis: BPS impaired the physical barrier, evoked inflammation, and resulted in dysbiosis of the gut microbiota. Additionally, BPS altered serum metabolites, including phosphatidic acid and diacylglycerol, which are involved in Fc gamma R-mediated phagocytosis and linked to inflammation. Furthermore, histopathological analysis, western blot (WB), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence results showed that exposure to BPS led to testicular damage, inflammation, activation of the p38 and ERK MAPK pathways, and disruption of the blood-testis barrier (BTB). Collectively, these findings indicate that BPS impair the intestinal health, disrupt gut microbiome, and ultimately lead to reproductive dysfunction through the gut-blood-testicular axis. |
| format | Article |
| id | doaj-art-bb8ffdabd6c74a549678c69c5138b467 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-bb8ffdabd6c74a549678c69c5138b4672025-01-23T05:25:57ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117646Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrityBiran Zhu0Zequan Zhang1Ying Xie2Min Huang3Yu Chen4Yong Yang5Xiongjie Shi6Jian Han7Lihua Yang8Min Zhao9School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, ChinaHubei Shizhen Laboratory, Wuhan 430061, China; School of Traditional Chinese Medicine, Hubei University of Chinese Medicine, Wuhan 430065, ChinaSpecAlly Life Technology Co., Ltd., Wuhan 430075, ChinaCollege of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Wuhan 430061, China; Corresponding author at: School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China.In this study, male Sprague–Dawley (SD) rats were exposed to bisphenol S (BPS) at environmentally relevant concentrations to investigate its reproductive toxicity and evaluate its effects on the gut-blood-testicular axis. After 28 days of exposure to BPS (0.05 and 20 mg/kg), the results showed a reduction in weight gain and the induction of reproductive toxicity in male rats, including decreased sperm parameters, lower sperm viability, and increased abnormal sperm density and mortality. These observations were made by counting with a hemocytometer under the optical microscope. 16S rRNA and untargeted metabolomic elucidated potential impacts on the gut-blood-testicular axis: BPS impaired the physical barrier, evoked inflammation, and resulted in dysbiosis of the gut microbiota. Additionally, BPS altered serum metabolites, including phosphatidic acid and diacylglycerol, which are involved in Fc gamma R-mediated phagocytosis and linked to inflammation. Furthermore, histopathological analysis, western blot (WB), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence results showed that exposure to BPS led to testicular damage, inflammation, activation of the p38 and ERK MAPK pathways, and disruption of the blood-testis barrier (BTB). Collectively, these findings indicate that BPS impair the intestinal health, disrupt gut microbiome, and ultimately lead to reproductive dysfunction through the gut-blood-testicular axis.http://www.sciencedirect.com/science/article/pii/S0147651324017226Bisphenol SReproductive toxicityIntestinal healthGut-blood-testicular axisInflammation |
| spellingShingle | Biran Zhu Zequan Zhang Ying Xie Min Huang Yu Chen Yong Yang Xiongjie Shi Jian Han Lihua Yang Min Zhao Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity Ecotoxicology and Environmental Safety Bisphenol S Reproductive toxicity Intestinal health Gut-blood-testicular axis Inflammation |
| title | Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity |
| title_full | Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity |
| title_fullStr | Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity |
| title_full_unstemmed | Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity |
| title_short | Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity |
| title_sort | effects of environmental bisphenol s exposure on male rat reproductive health and gut blood testicular axis integrity |
| topic | Bisphenol S Reproductive toxicity Intestinal health Gut-blood-testicular axis Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324017226 |
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