Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids
<i>Background and Objectives:</i> Insulin-like growth factor-1 (IGF-1) plays a vital role in various cellular processes, including those involving stem cells. This study evaluated the effects of IGF-1 on cell survival, osteogenic differentiation, and mRNA expression in gingiva-derived me...
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2025-01-01
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| author | Somyeong Hwa Hyun-Jin Lee Youngkyung Ko Jun-Beom Park |
| author_facet | Somyeong Hwa Hyun-Jin Lee Youngkyung Ko Jun-Beom Park |
| author_sort | Somyeong Hwa |
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| description | <i>Background and Objectives:</i> Insulin-like growth factor-1 (IGF-1) plays a vital role in various cellular processes, including those involving stem cells. This study evaluated the effects of IGF-1 on cell survival, osteogenic differentiation, and mRNA expression in gingiva-derived mesenchymal stem cell spheroids. <i>Materials and Methods:</i> Using concave microwells, spheroids were generated in the presence of IGF-1 at concentrations of 0, 10, and 100 ng/mL. Cellular vitality was qualitatively assessed using microscopy, while a water-soluble tetrazolium salt–based assay kit quantified cellular viability. Osteogenic differentiation was evaluated via alkaline phosphatase activity and an anthraquinone dye test to measure calcium deposition. Additionally, quantitative polymerase chain reaction (qPCR) analysis was performed to determine the expression of RUNX2 and COL1A1. <i>Results:</i> By day 1, the stem cell spheroids had successfully formed, and their morphology remained stable over the following 7 days. The IGF-1 concentrations tested showed no significant differences in cell viability. Similarly, alkaline phosphatase activity on day 7 revealed no observable changes. However, on day 7, the incorporation of IGF-1 led to an increase in Alizarin Red staining, indicative of enhanced calcium deposition. Notably, an IGF-1 concentration of 100 ng/mL significantly upregulated the expression of COL1A1. <i>Conclusions:</i> These findings suggest that IGF-1 supports the maintenance of cell viability and promotes the expression of COL1A1 in gingiva-derived mesenchymal stem cell spheroids, highlighting its potential role in enhancing osteogenic differentiation. Future research should include long-term studies to evaluate the sustainability of IGF-1-induced effects on stem cell spheroids. |
| format | Article |
| id | doaj-art-bb819e747de8428dac98f4e092b0a798 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Medicina |
| spelling | doaj-art-bb819e747de8428dac98f4e092b0a7982025-01-24T13:40:29ZengMDPI AGMedicina1010-660X1648-91442025-01-016117610.3390/medicina61010076Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell SpheroidsSomyeong Hwa0Hyun-Jin Lee1Youngkyung Ko2Jun-Beom Park3Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea<i>Background and Objectives:</i> Insulin-like growth factor-1 (IGF-1) plays a vital role in various cellular processes, including those involving stem cells. This study evaluated the effects of IGF-1 on cell survival, osteogenic differentiation, and mRNA expression in gingiva-derived mesenchymal stem cell spheroids. <i>Materials and Methods:</i> Using concave microwells, spheroids were generated in the presence of IGF-1 at concentrations of 0, 10, and 100 ng/mL. Cellular vitality was qualitatively assessed using microscopy, while a water-soluble tetrazolium salt–based assay kit quantified cellular viability. Osteogenic differentiation was evaluated via alkaline phosphatase activity and an anthraquinone dye test to measure calcium deposition. Additionally, quantitative polymerase chain reaction (qPCR) analysis was performed to determine the expression of RUNX2 and COL1A1. <i>Results:</i> By day 1, the stem cell spheroids had successfully formed, and their morphology remained stable over the following 7 days. The IGF-1 concentrations tested showed no significant differences in cell viability. Similarly, alkaline phosphatase activity on day 7 revealed no observable changes. However, on day 7, the incorporation of IGF-1 led to an increase in Alizarin Red staining, indicative of enhanced calcium deposition. Notably, an IGF-1 concentration of 100 ng/mL significantly upregulated the expression of COL1A1. <i>Conclusions:</i> These findings suggest that IGF-1 supports the maintenance of cell viability and promotes the expression of COL1A1 in gingiva-derived mesenchymal stem cell spheroids, highlighting its potential role in enhancing osteogenic differentiation. Future research should include long-term studies to evaluate the sustainability of IGF-1-induced effects on stem cell spheroids.https://www.mdpi.com/1648-9144/61/1/76cell survivalcell differentiationcellular spheroidsinsulin-like growth factor Iosteogenesisstem cells |
| spellingShingle | Somyeong Hwa Hyun-Jin Lee Youngkyung Ko Jun-Beom Park Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids Medicina cell survival cell differentiation cellular spheroids insulin-like growth factor I osteogenesis stem cells |
| title | Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids |
| title_full | Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids |
| title_fullStr | Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids |
| title_full_unstemmed | Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids |
| title_short | Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids |
| title_sort | effects of insulin like growth factor 1 on the maintenance of cell viability and osteogenic differentiation of gingiva derived mesenchymal stem cell spheroids |
| topic | cell survival cell differentiation cellular spheroids insulin-like growth factor I osteogenesis stem cells |
| url | https://www.mdpi.com/1648-9144/61/1/76 |
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