Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducks
The novel duck reovirus (NDRV) disease causes high economic losses, resulting in substantial economic losses in waterfowl industry. However, currently, no commercial vaccines are available to alleviate NDRV infection throughout the world. Here, we developed two subunit vaccine candidates for NDRV ba...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912401143X |
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| author | Yufei Wang Siyi Xu Xia Chen Yunlong Dou Xingzhu Yang Zenglei Hu Shuang Wu Xiaoquan Wang Jiao Hu Xiufan Liu |
| author_facet | Yufei Wang Siyi Xu Xia Chen Yunlong Dou Xingzhu Yang Zenglei Hu Shuang Wu Xiaoquan Wang Jiao Hu Xiufan Liu |
| author_sort | Yufei Wang |
| collection | DOAJ |
| description | The novel duck reovirus (NDRV) disease causes high economic losses, resulting in substantial economic losses in waterfowl industry. However, currently, no commercial vaccines are available to alleviate NDRV infection throughout the world. Here, we developed two subunit vaccine candidates for NDRV based on the insect cell-baculovirus expression system (IC-BEVS). Two recombinant viruses, namely rBac-σB and rBac-σC, were successfully generated based on the consensus sequence of NDRV. Then, the σB and σC subunit vaccine candidates were prepared by directly inactivating the recombinant virus infected-Sf9 cell suspension. The double antibody-sandwich ELISA was used for quantitative of σB or σC protein in the inactivated crude antigen. Protective efficacy results revealed that, compared with the whole virus inactivated vaccine, a single dose of 160 ng σB or σC protein showed advantages in inducing serum antibodies, elevating weight, alleviating liver and spleen injury, restraining viral shedding and viral replication in ducklings. To be noted, the subunit σC or the combination of subunit σB and σC vaccine candidates had better protective efficacies, especially the combined σB and σC vaccine group. Therefore, our study provides useful information for developing effective vaccine against NDRV infection. |
| format | Article |
| id | doaj-art-bb69f7c0ed944e2da917dec2d574d0eb |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-bb69f7c0ed944e2da917dec2d574d0eb2025-01-22T05:40:30ZengElsevierPoultry Science0032-57912025-01-011041104565Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducksYufei Wang0Siyi Xu1Xia Chen2Yunlong Dou3Xingzhu Yang4Zenglei Hu5Shuang Wu6Xiaoquan Wang7Jiao Hu8Xiufan Liu9Key Laboratory of Avian Bioproducts Developmen, Ministry of Agriculture and Rural Affairs, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Avian Bioproducts Developmen, Ministry of Agriculture and Rural Affairs, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Avian Bioproducts Developmen, Ministry of Agriculture and Rural Affairs, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Avian Bioproducts Developmen, Ministry of Agriculture and Rural Affairs, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Avian Bioproducts Developmen, Ministry of Agriculture and Rural Affairs, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, ChinaJiangsu Agri-animal Husbandry Vocational College, Taizhou, 225300, ChinaKey Laboratory of Avian Bioproducts Developmen, Ministry of Agriculture and Rural Affairs, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Avian Bioproducts Developmen, Ministry of Agriculture and Rural Affairs, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China; Corresponding author: Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, 48 East Wenhui Road, Yangzhou, Jiangsu Province 225009, China.Key Laboratory of Avian Bioproducts Developmen, Ministry of Agriculture and Rural Affairs, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, Jiangsu, ChinaThe novel duck reovirus (NDRV) disease causes high economic losses, resulting in substantial economic losses in waterfowl industry. However, currently, no commercial vaccines are available to alleviate NDRV infection throughout the world. Here, we developed two subunit vaccine candidates for NDRV based on the insect cell-baculovirus expression system (IC-BEVS). Two recombinant viruses, namely rBac-σB and rBac-σC, were successfully generated based on the consensus sequence of NDRV. Then, the σB and σC subunit vaccine candidates were prepared by directly inactivating the recombinant virus infected-Sf9 cell suspension. The double antibody-sandwich ELISA was used for quantitative of σB or σC protein in the inactivated crude antigen. Protective efficacy results revealed that, compared with the whole virus inactivated vaccine, a single dose of 160 ng σB or σC protein showed advantages in inducing serum antibodies, elevating weight, alleviating liver and spleen injury, restraining viral shedding and viral replication in ducklings. To be noted, the subunit σC or the combination of subunit σB and σC vaccine candidates had better protective efficacies, especially the combined σB and σC vaccine group. Therefore, our study provides useful information for developing effective vaccine against NDRV infection.http://www.sciencedirect.com/science/article/pii/S003257912401143XNDRVDucksσBσCSubunit vaccineIC-BEVS |
| spellingShingle | Yufei Wang Siyi Xu Xia Chen Yunlong Dou Xingzhu Yang Zenglei Hu Shuang Wu Xiaoquan Wang Jiao Hu Xiufan Liu Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducks Poultry Science NDRV Ducks σB σC Subunit vaccine IC-BEVS |
| title | Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducks |
| title_full | Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducks |
| title_fullStr | Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducks |
| title_full_unstemmed | Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducks |
| title_short | Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducks |
| title_sort | single dose of recombinant baculovirus vaccine expressing sigma b and sigma c genes provides good protection against novel duck reovirus challenge in ducks |
| topic | NDRV Ducks σB σC Subunit vaccine IC-BEVS |
| url | http://www.sciencedirect.com/science/article/pii/S003257912401143X |
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