An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture
A procedure for rapid clonal propagation of Passiflora edulis Sims. f. flavicarpa Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL−1 6-benzylaminopurine (BAP) to MS...
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Wiley
2015-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2015/578676 |
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| author | Mahipal S. Shekhawat M. Manokari C. P. Ravindran |
| author_facet | Mahipal S. Shekhawat M. Manokari C. P. Ravindran |
| author_sort | Mahipal S. Shekhawat |
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| description | A procedure for rapid clonal propagation of Passiflora edulis Sims. f. flavicarpa Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL−1 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21±1.13) primordial from the nodal meristems. Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL−1 of each BAP and Kinetin (Kin) was successful for the multiplication of the shoots in vitro with maximum numbers of shoots (25.73±0.06) within four weeks of incubation. Shoots were rooted best (7.13±0.56 roots/shoots) on half strength MS medium supplemented with 2.0 mgL−1 indole-3 butyric acid (IBA). All in vitro regenerated shoots were rooted by ex vitro method, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL−1 IBA. The plantlets were hardened in the greenhouse for 4-5 weeks. The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate. |
| format | Article |
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| language | English |
| publishDate | 2015-01-01 |
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| spelling | doaj-art-bb602892c68f4e8fa01747ffdfeaa8e62025-02-03T06:00:59ZengWileyScientifica2090-908X2015-01-01201510.1155/2015/578676578676An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment CultureMahipal S. Shekhawat0M. Manokari1C. P. Ravindran2Biotechnology Laboratory, Department of Plant Science, M.G.G.A.C., Mahe, Pondicherry 673311, IndiaBiotechnology Laboratory, Department of Plant Science, M.G.G.A.C., Mahe, Pondicherry 673311, IndiaBiotechnology Laboratory, Department of Plant Science, M.G.G.A.C., Mahe, Pondicherry 673311, IndiaA procedure for rapid clonal propagation of Passiflora edulis Sims. f. flavicarpa Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL−1 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21±1.13) primordial from the nodal meristems. Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL−1 of each BAP and Kinetin (Kin) was successful for the multiplication of the shoots in vitro with maximum numbers of shoots (25.73±0.06) within four weeks of incubation. Shoots were rooted best (7.13±0.56 roots/shoots) on half strength MS medium supplemented with 2.0 mgL−1 indole-3 butyric acid (IBA). All in vitro regenerated shoots were rooted by ex vitro method, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL−1 IBA. The plantlets were hardened in the greenhouse for 4-5 weeks. The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.http://dx.doi.org/10.1155/2015/578676 |
| spellingShingle | Mahipal S. Shekhawat M. Manokari C. P. Ravindran An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture Scientifica |
| title | An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture |
| title_full | An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture |
| title_fullStr | An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture |
| title_full_unstemmed | An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture |
| title_short | An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture |
| title_sort | improved micropropagation protocol by ex vitro rooting of passiflora edulis sims f flavicarpa deg through nodal segment culture |
| url | http://dx.doi.org/10.1155/2015/578676 |
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