Cryopreservation Techniques for Ram Sperm
Germplasm storage and transportation in artificial insemination (AI) and other advanced technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of sperm allows it to be transported across vast distances and used even after the sire’s death. However, the technique of c...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2022/7378379 |
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| author | Amit Saha Mohammad Asaduzzaman Farida Yeasmin Bari |
| author_facet | Amit Saha Mohammad Asaduzzaman Farida Yeasmin Bari |
| author_sort | Amit Saha |
| collection | DOAJ |
| description | Germplasm storage and transportation in artificial insemination (AI) and other advanced technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of sperm allows it to be transported across vast distances and used even after the sire’s death. However, the technique of cryopreservation might damage sperm and limit their activity. Several cryobiological investigations have reported that the integrity of the sperm membrane is frequently involved in the physical and biological elements that affect sperm survival at low temperatures during the cryopreservation process. However, successful cryopreservation of ram sperm is still a work in progress because a considerable percentage of sperm do not survive the freezing and thawing process. Sperms are destroyed during cryopreservation of semen due to varying concentrations of cryoprotective chemicals and if semen is not cooled at optimal cooling rates. Hence, it is crucial to know the optimum cooling rates with freezing and thawing protocols for maximum recovery of viable and functional sperm cells for a successful cryo-freezing of ram spermatozoa. Therefore, the current study compiled and compared the research on the impact of different cryopreservation procedures, cooling rates, equilibration time, and thawing protocols on post-thaw ram semen quality. |
| format | Article |
| id | doaj-art-bb4ac5a0388543f9b7eed04ae3419ef1 |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-bb4ac5a0388543f9b7eed04ae3419ef12025-02-03T01:22:57ZengWileyVeterinary Medicine International2042-00482022-01-01202210.1155/2022/7378379Cryopreservation Techniques for Ram SpermAmit Saha0Mohammad Asaduzzaman1Farida Yeasmin Bari2Department of Surgery and ObstetricsDepartment of Surgery and ObstetricsDepartment of Surgery and ObstetricsGermplasm storage and transportation in artificial insemination (AI) and other advanced technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of sperm allows it to be transported across vast distances and used even after the sire’s death. However, the technique of cryopreservation might damage sperm and limit their activity. Several cryobiological investigations have reported that the integrity of the sperm membrane is frequently involved in the physical and biological elements that affect sperm survival at low temperatures during the cryopreservation process. However, successful cryopreservation of ram sperm is still a work in progress because a considerable percentage of sperm do not survive the freezing and thawing process. Sperms are destroyed during cryopreservation of semen due to varying concentrations of cryoprotective chemicals and if semen is not cooled at optimal cooling rates. Hence, it is crucial to know the optimum cooling rates with freezing and thawing protocols for maximum recovery of viable and functional sperm cells for a successful cryo-freezing of ram spermatozoa. Therefore, the current study compiled and compared the research on the impact of different cryopreservation procedures, cooling rates, equilibration time, and thawing protocols on post-thaw ram semen quality.http://dx.doi.org/10.1155/2022/7378379 |
| spellingShingle | Amit Saha Mohammad Asaduzzaman Farida Yeasmin Bari Cryopreservation Techniques for Ram Sperm Veterinary Medicine International |
| title | Cryopreservation Techniques for Ram Sperm |
| title_full | Cryopreservation Techniques for Ram Sperm |
| title_fullStr | Cryopreservation Techniques for Ram Sperm |
| title_full_unstemmed | Cryopreservation Techniques for Ram Sperm |
| title_short | Cryopreservation Techniques for Ram Sperm |
| title_sort | cryopreservation techniques for ram sperm |
| url | http://dx.doi.org/10.1155/2022/7378379 |
| work_keys_str_mv | AT amitsaha cryopreservationtechniquesforramsperm AT mohammadasaduzzaman cryopreservationtechniquesforramsperm AT faridayeasminbari cryopreservationtechniquesforramsperm |