Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice
BackgroundInflammatory bowel disease (IBD) has become a global public health problem with complex pathogenesis and limited therapeutic options. We aimed to investigate the potential mechanisms by which Bifidobacterium lactis V9 (V9) alleviated colitis in a dextran sodium sulfate-induced colitis mode...
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Frontiers Media S.A.
2025-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1496023/full |
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| author | Xiaoyan Duan Xiaoyan Duan Xiaoyan Duan Xiaoyan Duan Rilige Wu Jianbo Li Jianbo Li Zeya Li Yanqi Liu Ping Chen Bangmao Wang Bangmao Wang Bangmao Wang |
| author_facet | Xiaoyan Duan Xiaoyan Duan Xiaoyan Duan Xiaoyan Duan Rilige Wu Jianbo Li Jianbo Li Zeya Li Yanqi Liu Ping Chen Bangmao Wang Bangmao Wang Bangmao Wang |
| author_sort | Xiaoyan Duan |
| collection | DOAJ |
| description | BackgroundInflammatory bowel disease (IBD) has become a global public health problem with complex pathogenesis and limited therapeutic options. We aimed to investigate the potential mechanisms by which Bifidobacterium lactis V9 (V9) alleviated colitis in a dextran sodium sulfate-induced colitis model mice.MethodsMice were induced to develop colitis by drinking DSS solution to induce colitis. The expression of the relevant factors in the blood supernatant of the mice was determined by ELISA. RT-qPCR and Western blotting were used to detect mRNA and protein expression of target genes. The fecal microbiota was analyzed by 16S rRNA sequencing. Intestinal metabolites were analyzed by untargeted metabolomics;ResultsV9 effectively improved the overall symptoms of the colitis model mice. H&E showed that V9 re-stored the intestinal tissue structure. ELISA showed that V9 decreased the levels of IL-6, IL-22, and TNF-α and increased IL-10, SP, VIP, and 5-HT. V9 increased the expression of AHR, CYP1A1, MUC2, Claudin-3, Occludin, and ZO-1, and decreased 5-hydroxytryptamine transporter and Claudin-2. V9 increased the abundance of gut microbiota in colitis mice to promote the growth of beneficial bacteria. V9 increased tryptophan metabolites, and short-chain fatty acids, and improved gut inflammation.ConclusionV9 attenuates intestinal inflammation, improves the mucosal barrier, modulates intestinal microecology and exerts a protective effect in a mouse model of DSS-induced colitis. |
| format | Article |
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| institution | Kabale University |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj-art-bb497e4290bc4c53933930e2a0debc892025-01-24T07:13:43ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-01-011210.3389/fmed.2025.14960231496023Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in miceXiaoyan Duan0Xiaoyan Duan1Xiaoyan Duan2Xiaoyan Duan3Rilige Wu4Jianbo Li5Jianbo Li6Zeya Li7Yanqi Liu8Ping Chen9Bangmao Wang10Bangmao Wang11Bangmao Wang12Department of Gastroenterology, General Hospital, Tianjin Medical University, Tianjin, ChinaTianjin Institute of Digestive Diseases, Tianjin, ChinaTianjin Key Laboratory of Digestive Diseases, Tianjin, ChinaDepartment of Gastroenterology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Gastroenterology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Nuclear Medicine, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaInner Mongolia Key Laboratory of Molecular Imaging, Hohhot, ChinaDepartment of Gastroenterology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Gastroenterology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Gastroenterology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Gastroenterology, General Hospital, Tianjin Medical University, Tianjin, ChinaTianjin Institute of Digestive Diseases, Tianjin, ChinaTianjin Key Laboratory of Digestive Diseases, Tianjin, ChinaBackgroundInflammatory bowel disease (IBD) has become a global public health problem with complex pathogenesis and limited therapeutic options. We aimed to investigate the potential mechanisms by which Bifidobacterium lactis V9 (V9) alleviated colitis in a dextran sodium sulfate-induced colitis model mice.MethodsMice were induced to develop colitis by drinking DSS solution to induce colitis. The expression of the relevant factors in the blood supernatant of the mice was determined by ELISA. RT-qPCR and Western blotting were used to detect mRNA and protein expression of target genes. The fecal microbiota was analyzed by 16S rRNA sequencing. Intestinal metabolites were analyzed by untargeted metabolomics;ResultsV9 effectively improved the overall symptoms of the colitis model mice. H&E showed that V9 re-stored the intestinal tissue structure. ELISA showed that V9 decreased the levels of IL-6, IL-22, and TNF-α and increased IL-10, SP, VIP, and 5-HT. V9 increased the expression of AHR, CYP1A1, MUC2, Claudin-3, Occludin, and ZO-1, and decreased 5-hydroxytryptamine transporter and Claudin-2. V9 increased the abundance of gut microbiota in colitis mice to promote the growth of beneficial bacteria. V9 increased tryptophan metabolites, and short-chain fatty acids, and improved gut inflammation.ConclusionV9 attenuates intestinal inflammation, improves the mucosal barrier, modulates intestinal microecology and exerts a protective effect in a mouse model of DSS-induced colitis.https://www.frontiersin.org/articles/10.3389/fmed.2025.1496023/fullBifidobacterium lactis V9ulcerative colitisintestinal barrierintestinal floratryptophan metabolism |
| spellingShingle | Xiaoyan Duan Xiaoyan Duan Xiaoyan Duan Xiaoyan Duan Rilige Wu Jianbo Li Jianbo Li Zeya Li Yanqi Liu Ping Chen Bangmao Wang Bangmao Wang Bangmao Wang Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice Frontiers in Medicine Bifidobacterium lactis V9 ulcerative colitis intestinal barrier intestinal flora tryptophan metabolism |
| title | Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice |
| title_full | Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice |
| title_fullStr | Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice |
| title_full_unstemmed | Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice |
| title_short | Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice |
| title_sort | studies on the alleviating effect of bifidobacterium lactis v9 on dextran sodium sulfate induced colitis in mice |
| topic | Bifidobacterium lactis V9 ulcerative colitis intestinal barrier intestinal flora tryptophan metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1496023/full |
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