Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process
We aimed to establish a quantitative analysis method of six constituents (5-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) in Artemisia capillaris (Yinchen) and its decoction by using HPLC couple...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8950324 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550163188547584 |
|---|---|
| author | Fang Tian Qun-Jia Ruan Ying Zhang Hui Cao Zhi-Guo Ma Gai-Lian Zhou Meng-Hua Wu |
| author_facet | Fang Tian Qun-Jia Ruan Ying Zhang Hui Cao Zhi-Guo Ma Gai-Lian Zhou Meng-Hua Wu |
| author_sort | Fang Tian |
| collection | DOAJ |
| description | We aimed to establish a quantitative analysis method of six constituents (5-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) in Artemisia capillaris (Yinchen) and its decoction by using HPLC coupled with DAD. Besides, the transformation paths of the six constituents were analyzed in decoction preparation processing. The analytical method was fully validated in terms of linearity, sensitivity, precision, repeatability, and recovery and applied to assess the transformation trend and quantitative analysis of the six constituents in Yinchen decoction. The contents of six constituents varied greatly in Yinchen herb and Yinchen decoction, and there were inextricable internal relationships between them. Presumably 3-caffeoylquinic acid was isomerized to generate 5-caffeoylquinic acid and 4-caffeoylquinic acid. Similarly, 1,3-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid were produced by isomerization of 4,5-dicaffeoylquinic acid. In conclusion, this study provides a chemical basis for quality control of Yinchen decoction, and the changes of selected markers in decoction could give us some novel perspectives to study the relationship between substances and drug efficacy. |
| format | Article |
| id | doaj-art-bb1fd3245f254074b68eb488e7aafaa5 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-bb1fd3245f254074b68eb488e7aafaa52025-02-03T06:07:38ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/89503248950324Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation ProcessFang Tian0Qun-Jia Ruan1Ying Zhang2Hui Cao3Zhi-Guo Ma4Gai-Lian Zhou5Meng-Hua Wu6College of Pharmacy, Jinan University, Guangzhou, ChinaCollege of Pharmacy, Jinan University, Guangzhou, ChinaCollege of Pharmacy, Jinan University, Guangzhou, ChinaCollege of Pharmacy, Jinan University, Guangzhou, ChinaCollege of Pharmacy, Jinan University, Guangzhou, ChinaGuangxi University of Chinese Medicine, Nanning, ChinaCollege of Pharmacy, Jinan University, Guangzhou, ChinaWe aimed to establish a quantitative analysis method of six constituents (5-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) in Artemisia capillaris (Yinchen) and its decoction by using HPLC coupled with DAD. Besides, the transformation paths of the six constituents were analyzed in decoction preparation processing. The analytical method was fully validated in terms of linearity, sensitivity, precision, repeatability, and recovery and applied to assess the transformation trend and quantitative analysis of the six constituents in Yinchen decoction. The contents of six constituents varied greatly in Yinchen herb and Yinchen decoction, and there were inextricable internal relationships between them. Presumably 3-caffeoylquinic acid was isomerized to generate 5-caffeoylquinic acid and 4-caffeoylquinic acid. Similarly, 1,3-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid were produced by isomerization of 4,5-dicaffeoylquinic acid. In conclusion, this study provides a chemical basis for quality control of Yinchen decoction, and the changes of selected markers in decoction could give us some novel perspectives to study the relationship between substances and drug efficacy.http://dx.doi.org/10.1155/2020/8950324 |
| spellingShingle | Fang Tian Qun-Jia Ruan Ying Zhang Hui Cao Zhi-Guo Ma Gai-Lian Zhou Meng-Hua Wu Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process Journal of Analytical Methods in Chemistry |
| title | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
| title_full | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
| title_fullStr | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
| title_full_unstemmed | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
| title_short | Quantitative Analysis of Six Phenolic Acids in Artemisia capillaris (Yinchen) by HPLC-DAD and Their Transformation Pathways in Decoction Preparation Process |
| title_sort | quantitative analysis of six phenolic acids in artemisia capillaris yinchen by hplc dad and their transformation pathways in decoction preparation process |
| url | http://dx.doi.org/10.1155/2020/8950324 |
| work_keys_str_mv | AT fangtian quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT qunjiaruan quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT yingzhang quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT huicao quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT zhiguoma quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT gailianzhou quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess AT menghuawu quantitativeanalysisofsixphenolicacidsinartemisiacapillarisyinchenbyhplcdadandtheirtransformationpathwaysindecoctionpreparationprocess |