DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS
The 3AB-ELISA test-system for detection of antibodies to FMD virus nonstructural proteins in blood sera from cattle and small ruminants was validated. It was discovered that the reaction repeatability and reproducibility exceeded 90%, diagnostic specificity constituted 99,8%, diagnostic sensitivity...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/217 |
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| author | A. S. Yakovleva A. V. Kanshina A. V. Scherbakov Ye. S. Orlova |
| author_facet | A. S. Yakovleva A. V. Kanshina A. V. Scherbakov Ye. S. Orlova |
| author_sort | A. S. Yakovleva |
| collection | DOAJ |
| description | The 3AB-ELISA test-system for detection of antibodies to FMD virus nonstructural proteins in blood sera from cattle and small ruminants was validated. It was discovered that the reaction repeatability and reproducibility exceeded 90%, diagnostic specificity constituted 99,8%, diagnostic sensitivity came up to 96,6%. The high specificity and sensitivity of the 3AB-ELISA test-system was confirmed by international proficiency testings as far as FMD diagnostics and by routine diagnostic and monitoring studies. |
| format | Article |
| id | doaj-art-bb0447082844435d882636cc1c9a2de2 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-bb0447082844435d882636cc1c9a2de22025-02-06T09:52:05ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-01043642217DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTSA. S. Yakovleva0A. V. Kanshina1A. V. Scherbakov2Ye. S. Orlova3FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirThe 3AB-ELISA test-system for detection of antibodies to FMD virus nonstructural proteins in blood sera from cattle and small ruminants was validated. It was discovered that the reaction repeatability and reproducibility exceeded 90%, diagnostic specificity constituted 99,8%, diagnostic sensitivity came up to 96,6%. The high specificity and sensitivity of the 3AB-ELISA test-system was confirmed by international proficiency testings as far as FMD diagnostics and by routine diagnostic and monitoring studies.https://veterinary.arriah.ru/jour/article/view/217вирус ящуранеструктурные белкииммуноферментный анализвалидацияfmd virusnonstructural proteinsenzyme-linked immunosorbent assayvalidation |
| spellingShingle | A. S. Yakovleva A. V. Kanshina A. V. Scherbakov Ye. S. Orlova DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS Ветеринария сегодня вирус ящура неструктурные белки иммуноферментный анализ валидация fmd virus nonstructural proteins enzyme-linked immunosorbent assay validation |
| title | DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS |
| title_full | DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS |
| title_fullStr | DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS |
| title_full_unstemmed | DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS |
| title_short | DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS |
| title_sort | development and validation of 3ab elisatest system for detection of antibodies to fmd virus nonstructural proteins in blood sera from cattle and small ruminants |
| topic | вирус ящура неструктурные белки иммуноферментный анализ валидация fmd virus nonstructural proteins enzyme-linked immunosorbent assay validation |
| url | https://veterinary.arriah.ru/jour/article/view/217 |
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