RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts
Abstract RPOTp is the nuclear‐encoded plastid‐targeted RNA polymerase and plays a crucial role in chloroplast gene expression. Transcripts in plant organelles are altered by the conversion of cytidine (C) to uridine (U) at specific positions through RNA editing. However, whether RPOTp is involved in...
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202405131 |
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| author | Nadia Ahmed Ali Wenjian Song Yayi Zhang Jiani Xing Kexing Su Xingxing Sun Yujia Sun Yizhou Jiang Dianxing Wu Xiaobo Zhao |
| author_facet | Nadia Ahmed Ali Wenjian Song Yayi Zhang Jiani Xing Kexing Su Xingxing Sun Yujia Sun Yizhou Jiang Dianxing Wu Xiaobo Zhao |
| author_sort | Nadia Ahmed Ali |
| collection | DOAJ |
| description | Abstract RPOTp is the nuclear‐encoded plastid‐targeted RNA polymerase and plays a crucial role in chloroplast gene expression. Transcripts in plant organelles are altered by the conversion of cytidine (C) to uridine (U) at specific positions through RNA editing. However, whether RPOTp is involved in chloroplast RNA editing remains unclear. Here, the role of RPOTp in C‐to‐U RNA editing at multiple sites in Arabidopsis chloroplasts is uncovered. Multiple organellar RNA editing factor 2 (MORF2) is required for the editing of most sites in chloroplasts. RPOTp is identified from the co‐immunoprecipitation targets of MORF2. The sca3‐2 mutant, defective in RPOTp, exhibits a pale‐yellow phenotype and alters the RNA editing of nine sites in chloroplasts. It is also shown that RNA editing is uncoupled from chloroplast transcriptional activity. RPOTp directly interacts with chloroplast multiple‐site RNA editing factors, including MORF2, MORF8, MORF9, and ORRM1. It is further shown that RPOTp participates in RNA editing by influencing the dimerization of MORF proteins. The defect in RPOTp impairs the expression of most chloroplast genes, indicating an indispensable role for RPOTp in chloroplast gene expression. These findings reveal that RPOTp not only participates in transcription but also has a novel role in RNA editing of chloroplast transcripts. |
| format | Article |
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| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Advanced Science |
| spelling | doaj-art-bae045b73b674d97b253603e4c85d55e2025-01-29T09:50:19ZengWileyAdvanced Science2198-38442025-01-01124n/an/a10.1002/advs.202405131RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis ChloroplastsNadia Ahmed Ali0Wenjian Song1Yayi Zhang2Jiani Xing3Kexing Su4Xingxing Sun5Yujia Sun6Yizhou Jiang7Dianxing Wu8Xiaobo Zhao9Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaKey Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaKey Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaKey Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaKey Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaKey Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaKey Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaKey Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaKey Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaKey Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs Key Laboratory of Nuclear Agricultural Sciences of Zhejiang Province Institute of Nuclear Agricultural Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 ChinaAbstract RPOTp is the nuclear‐encoded plastid‐targeted RNA polymerase and plays a crucial role in chloroplast gene expression. Transcripts in plant organelles are altered by the conversion of cytidine (C) to uridine (U) at specific positions through RNA editing. However, whether RPOTp is involved in chloroplast RNA editing remains unclear. Here, the role of RPOTp in C‐to‐U RNA editing at multiple sites in Arabidopsis chloroplasts is uncovered. Multiple organellar RNA editing factor 2 (MORF2) is required for the editing of most sites in chloroplasts. RPOTp is identified from the co‐immunoprecipitation targets of MORF2. The sca3‐2 mutant, defective in RPOTp, exhibits a pale‐yellow phenotype and alters the RNA editing of nine sites in chloroplasts. It is also shown that RNA editing is uncoupled from chloroplast transcriptional activity. RPOTp directly interacts with chloroplast multiple‐site RNA editing factors, including MORF2, MORF8, MORF9, and ORRM1. It is further shown that RPOTp participates in RNA editing by influencing the dimerization of MORF proteins. The defect in RPOTp impairs the expression of most chloroplast genes, indicating an indispensable role for RPOTp in chloroplast gene expression. These findings reveal that RPOTp not only participates in transcription but also has a novel role in RNA editing of chloroplast transcripts.https://doi.org/10.1002/advs.202405131chloroplastMORFRNA editingRNA polymeraseRPOTp |
| spellingShingle | Nadia Ahmed Ali Wenjian Song Yayi Zhang Jiani Xing Kexing Su Xingxing Sun Yujia Sun Yizhou Jiang Dianxing Wu Xiaobo Zhao RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts Advanced Science chloroplast MORF RNA editing RNA polymerase RPOTp |
| title | RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts |
| title_full | RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts |
| title_fullStr | RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts |
| title_full_unstemmed | RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts |
| title_short | RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts |
| title_sort | rna polymerase rpotp is involved in c to u rna editing at multiple sites in arabidopsis chloroplasts |
| topic | chloroplast MORF RNA editing RNA polymerase RPOTp |
| url | https://doi.org/10.1002/advs.202405131 |
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