Saliva-acquired pellicle inspired multifunctional gargle with wet adhesion, photodynamic antimicrobial, and In situ remineralization properties for dental caries prevention
Dental caries is primarily caused by cariogenic bacteria metabolizing carbohydrates to produce acidic substances that erode the dental hard tissues. Traditional remineralization treatments often have limited efficacy due to their lack of antibacterial activity. According to the Interrupting Dental C...
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KeAi Communications Co., Ltd.
2025-05-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X25000088 |
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| author | Jiayi Shi Xuekai Qi Ying Ran Qiang Zhou Yiqin Ding Lujian Li Youyun Zeng Dongchao Qiu Zhibin Cai Xiaojun Cai Yihuai Pan |
| author_facet | Jiayi Shi Xuekai Qi Ying Ran Qiang Zhou Yiqin Ding Lujian Li Youyun Zeng Dongchao Qiu Zhibin Cai Xiaojun Cai Yihuai Pan |
| author_sort | Jiayi Shi |
| collection | DOAJ |
| description | Dental caries is primarily caused by cariogenic bacteria metabolizing carbohydrates to produce acidic substances that erode the dental hard tissues. Traditional remineralization treatments often have limited efficacy due to their lack of antibacterial activity. According to the Interrupting Dental Caries (IDC) theory, ideal caries-preventive materials should possess both antibacterial and remineralizing properties. Furthermore, effective adhesion to dental surfaces is crucial. Inspired by the wet adhesion properties of the salivary acquired pellicle, we developed a multifunctional gargle named Ce6@PDN-SAP (CP-SAP). This formulation employed peptide dendrimer nanogels (PDN) as a carrier for the photosensitizer Ce6, further functionalized with saliva-acquired peptide (SAP) to confer wet adhesion properties. CP-SAP rapidly adhered to the dental surface and remained effective for extended periods. Upon laser irradiation, Ce6 generated reactive oxygen species (ROS), disrupting bacterial outer membrane integrity, causing protein leakage, and reducing ATP levels, thereby achieving potent antibacterial effects. Following this, PDN and SAP acted as nucleation templates to promote in situ remineralization of demineralized dental hard tissues. In vivo studies using rat models demonstrated that CP-SAP provided significantly superior caries-preventive effects compared to chlorhexidine gargle. In conclusion, CP-SAP, as an innovative approach grounded in the IDC theory, holds great promise for the prevention and treatment of dental caries. |
| format | Article |
| id | doaj-art-bac5629c4765423c902af90638b02260 |
| institution | Kabale University |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-bac5629c4765423c902af90638b022602025-01-24T04:45:32ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-05-0147212228Saliva-acquired pellicle inspired multifunctional gargle with wet adhesion, photodynamic antimicrobial, and In situ remineralization properties for dental caries preventionJiayi Shi0Xuekai Qi1Ying Ran2Qiang Zhou3Yiqin Ding4Lujian Li5Youyun Zeng6Dongchao Qiu7Zhibin Cai8Xiaojun Cai9Yihuai Pan10School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, ChinaThe Third Affiliated Hospital of Wenzhou Medical University (Ruian People's Hospital), Wenzhou, 325016, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China; Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, ChinaSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, ChinaDepartment of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China; Corresponding author.School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China; Corresponding author.School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China; Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China; Corresponding author. School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China.Dental caries is primarily caused by cariogenic bacteria metabolizing carbohydrates to produce acidic substances that erode the dental hard tissues. Traditional remineralization treatments often have limited efficacy due to their lack of antibacterial activity. According to the Interrupting Dental Caries (IDC) theory, ideal caries-preventive materials should possess both antibacterial and remineralizing properties. Furthermore, effective adhesion to dental surfaces is crucial. Inspired by the wet adhesion properties of the salivary acquired pellicle, we developed a multifunctional gargle named Ce6@PDN-SAP (CP-SAP). This formulation employed peptide dendrimer nanogels (PDN) as a carrier for the photosensitizer Ce6, further functionalized with saliva-acquired peptide (SAP) to confer wet adhesion properties. CP-SAP rapidly adhered to the dental surface and remained effective for extended periods. Upon laser irradiation, Ce6 generated reactive oxygen species (ROS), disrupting bacterial outer membrane integrity, causing protein leakage, and reducing ATP levels, thereby achieving potent antibacterial effects. Following this, PDN and SAP acted as nucleation templates to promote in situ remineralization of demineralized dental hard tissues. In vivo studies using rat models demonstrated that CP-SAP provided significantly superior caries-preventive effects compared to chlorhexidine gargle. In conclusion, CP-SAP, as an innovative approach grounded in the IDC theory, holds great promise for the prevention and treatment of dental caries.http://www.sciencedirect.com/science/article/pii/S2452199X25000088Saliva-acquired pellicleWet adhesionTooth remineralizationAntimicrobial photodynamic therapyCaries preventionInterrupting dental caries |
| spellingShingle | Jiayi Shi Xuekai Qi Ying Ran Qiang Zhou Yiqin Ding Lujian Li Youyun Zeng Dongchao Qiu Zhibin Cai Xiaojun Cai Yihuai Pan Saliva-acquired pellicle inspired multifunctional gargle with wet adhesion, photodynamic antimicrobial, and In situ remineralization properties for dental caries prevention Bioactive Materials Saliva-acquired pellicle Wet adhesion Tooth remineralization Antimicrobial photodynamic therapy Caries prevention Interrupting dental caries |
| title | Saliva-acquired pellicle inspired multifunctional gargle with wet adhesion, photodynamic antimicrobial, and In situ remineralization properties for dental caries prevention |
| title_full | Saliva-acquired pellicle inspired multifunctional gargle with wet adhesion, photodynamic antimicrobial, and In situ remineralization properties for dental caries prevention |
| title_fullStr | Saliva-acquired pellicle inspired multifunctional gargle with wet adhesion, photodynamic antimicrobial, and In situ remineralization properties for dental caries prevention |
| title_full_unstemmed | Saliva-acquired pellicle inspired multifunctional gargle with wet adhesion, photodynamic antimicrobial, and In situ remineralization properties for dental caries prevention |
| title_short | Saliva-acquired pellicle inspired multifunctional gargle with wet adhesion, photodynamic antimicrobial, and In situ remineralization properties for dental caries prevention |
| title_sort | saliva acquired pellicle inspired multifunctional gargle with wet adhesion photodynamic antimicrobial and in situ remineralization properties for dental caries prevention |
| topic | Saliva-acquired pellicle Wet adhesion Tooth remineralization Antimicrobial photodynamic therapy Caries prevention Interrupting dental caries |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X25000088 |
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