Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation
Coprinus comatus polysaccharide (CCP) has significant hepatoprotective effect. To explore hepatoprotective mechanism of CCP, the study analyzed preventive effect of CCP on acute alcoholic liver injury in mice by histopathological examination and biochemical analysis. Simultaneously, hepatoprotective...
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Tsinghua University Press
2024-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001209 |
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| author | Jinyan Yu Jianguang Sun Min Sun Weidong Li Dongmei Qi Yongqing Zhang Chunchao Han |
| author_facet | Jinyan Yu Jianguang Sun Min Sun Weidong Li Dongmei Qi Yongqing Zhang Chunchao Han |
| author_sort | Jinyan Yu |
| collection | DOAJ |
| description | Coprinus comatus polysaccharide (CCP) has significant hepatoprotective effect. To explore hepatoprotective mechanism of CCP, the study analyzed preventive effect of CCP on acute alcoholic liver injury in mice by histopathological examination and biochemical analysis. Simultaneously, hepatoprotective mechanism was also analyzed in conjunction with metabolomics and proliferation of gut microbiota. The results showed that CCP significantly decreased alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) levels in serum of alcoholic liver disease (ALD) mice. Histopathological examination showed that CCP can significantly improve liver damage. Metabolomics results showed that there were significant differences in the level of metabolites in liver tissue of control group, ALD group and CCP group, including taurine, xanthosine, fumaric acid and arachidonic acid, among others. Metabolites pathways analysis showed that hepatoprotective effect of CCP was related to energy metabolism, biosynthesis of unsaturated fatty acids, amino acids metabolism and lipid metabolism. Additionally, CCP inhibited an increase in the number of Clostridium perfringens, Enterobacteriaceae and Enterococcus, and a decrease in the number of Lactobacillus and Bifidobacterium in the gut of ALD mice. All these findings suggested that CCP treatment reversed the phenotype of ethanol-induced liver injury and the associated metabolites pathways. |
| format | Article |
| id | doaj-art-bac47f754ec9429da63b2eb8a6712e5b |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-bac47f754ec9429da63b2eb8a6712e5b2025-02-03T04:09:17ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131401413Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigationJinyan Yu0Jianguang Sun1Min Sun2Weidong Li3Dongmei Qi4Yongqing Zhang5Chunchao Han6School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, ChinaHepatology Department of Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250355, ChinaSchool of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, ChinaSchool of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, ChinaExperimental center, Shandong University of Traditional Chinese Medicine, Jinan, 250355, ChinaSchool of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, ChinaSchool of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China; Corresponding author at: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.Coprinus comatus polysaccharide (CCP) has significant hepatoprotective effect. To explore hepatoprotective mechanism of CCP, the study analyzed preventive effect of CCP on acute alcoholic liver injury in mice by histopathological examination and biochemical analysis. Simultaneously, hepatoprotective mechanism was also analyzed in conjunction with metabolomics and proliferation of gut microbiota. The results showed that CCP significantly decreased alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) levels in serum of alcoholic liver disease (ALD) mice. Histopathological examination showed that CCP can significantly improve liver damage. Metabolomics results showed that there were significant differences in the level of metabolites in liver tissue of control group, ALD group and CCP group, including taurine, xanthosine, fumaric acid and arachidonic acid, among others. Metabolites pathways analysis showed that hepatoprotective effect of CCP was related to energy metabolism, biosynthesis of unsaturated fatty acids, amino acids metabolism and lipid metabolism. Additionally, CCP inhibited an increase in the number of Clostridium perfringens, Enterobacteriaceae and Enterococcus, and a decrease in the number of Lactobacillus and Bifidobacterium in the gut of ALD mice. All these findings suggested that CCP treatment reversed the phenotype of ethanol-induced liver injury and the associated metabolites pathways.http://www.sciencedirect.com/science/article/pii/S2213453023001209Coprinus comatusPolysaccharideAlcoholic liver diseaseMetabolomicsGut microbiota |
| spellingShingle | Jinyan Yu Jianguang Sun Min Sun Weidong Li Dongmei Qi Yongqing Zhang Chunchao Han Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation Food Science and Human Wellness Coprinus comatus Polysaccharide Alcoholic liver disease Metabolomics Gut microbiota |
| title | Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation |
| title_full | Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation |
| title_fullStr | Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation |
| title_full_unstemmed | Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation |
| title_short | Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation |
| title_sort | protective mechanism of coprinus comatus polysaccharide on acute alcoholic liver injury in mice the metabolomics and gut microbiota investigation |
| topic | Coprinus comatus Polysaccharide Alcoholic liver disease Metabolomics Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001209 |
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