Integrated approach to explore Anemonia viridis regeneration under a climate change scenario
Abstract This study investigates the mechanisms of regeneration in Anemonia viridis under natural conditions and thermal stress, addressing the question: “Does an anthozoan subjected to thermal stress regenerate similarly to one experiencing only a wound?“. Oxidative stress markers (protein carbonyl...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-11041-7 |
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| author | Claudia La Corte Stephanie Barnay-Verdier Paola Furla Luca Bisanti Mariano Dara Gabriele Rizzuto Salvatrice Vizzini Maria Giovanna Parisi Matteo Cammarata |
| author_facet | Claudia La Corte Stephanie Barnay-Verdier Paola Furla Luca Bisanti Mariano Dara Gabriele Rizzuto Salvatrice Vizzini Maria Giovanna Parisi Matteo Cammarata |
| author_sort | Claudia La Corte |
| collection | DOAJ |
| description | Abstract This study investigates the mechanisms of regeneration in Anemonia viridis under natural conditions and thermal stress, addressing the question: “Does an anthozoan subjected to thermal stress regenerate similarly to one experiencing only a wound?“. Oxidative stress markers (protein carbonylation, total antioxidant capacity) and symbiont photosynthetic efficiency (via Pulse Amplitude Modulation) were analyzed. Key proteins related to inflammation and tissue regeneration, including toll-like receptor, nuclear factor kappa B, heat shock proteins, and interleukin-1β, were examined using blotting techniques. Observations revealed higher antioxidant capacity at 20 °C than 27 °C after 6- and 24-hours post-injury. Thermal stress disrupted redox balance, as indicated by decreased symbiont photosynthetic efficiency. Protein expression analyses (proliferating cell nuclear antigen, heat shock protein 90, collagen Type XXIV α1) showed activation of compensatory mechanisms, but oxidative stress biomarkers highlighted significant cellular stress. These results suggest that elevated temperatures may impair regeneration in Anemonia viridis, highlighting a potential vulnerability of anthozoans to thermal stress associated with climate change. |
| format | Article |
| id | doaj-art-bab3b5c1c18f4c1da69b0c2a1b5bcd4c |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-bab3b5c1c18f4c1da69b0c2a1b5bcd4c2025-08-20T03:04:30ZengNature PortfolioScientific Reports2045-23222025-07-0115111610.1038/s41598-025-11041-7Integrated approach to explore Anemonia viridis regeneration under a climate change scenarioClaudia La Corte0Stephanie Barnay-Verdier1Paola Furla2Luca Bisanti3Mariano Dara4Gabriele Rizzuto5Salvatrice Vizzini6Maria Giovanna Parisi7Matteo Cammarata8Marine Immunobiology Laboratory, Department of Earth and Marine Sciences (DiSTeM), University of PalermoSorbonne Université UFR 927LIA ROPSE, Laboratoire International Associé Université Côte d’Azur-Centre Scientifique de MonacoMarine Immunobiology Laboratory, Department of Earth and Marine Sciences (DiSTeM), University of PalermoMarine Immunobiology Laboratory, Department of Earth and Marine Sciences (DiSTeM), University of PalermoDepartment of Earth and Marine Sciences (DiSTeM), University of PalermoDepartment of Earth and Marine Sciences (DiSTeM), University of PalermoMarine Immunobiology Laboratory, Department of Earth and Marine Sciences (DiSTeM), University of PalermoMarine Immunobiology Laboratory, Department of Earth and Marine Sciences (DiSTeM), University of PalermoAbstract This study investigates the mechanisms of regeneration in Anemonia viridis under natural conditions and thermal stress, addressing the question: “Does an anthozoan subjected to thermal stress regenerate similarly to one experiencing only a wound?“. Oxidative stress markers (protein carbonylation, total antioxidant capacity) and symbiont photosynthetic efficiency (via Pulse Amplitude Modulation) were analyzed. Key proteins related to inflammation and tissue regeneration, including toll-like receptor, nuclear factor kappa B, heat shock proteins, and interleukin-1β, were examined using blotting techniques. Observations revealed higher antioxidant capacity at 20 °C than 27 °C after 6- and 24-hours post-injury. Thermal stress disrupted redox balance, as indicated by decreased symbiont photosynthetic efficiency. Protein expression analyses (proliferating cell nuclear antigen, heat shock protein 90, collagen Type XXIV α1) showed activation of compensatory mechanisms, but oxidative stress biomarkers highlighted significant cellular stress. These results suggest that elevated temperatures may impair regeneration in Anemonia viridis, highlighting a potential vulnerability of anthozoans to thermal stress associated with climate change.https://doi.org/10.1038/s41598-025-11041-7Thermal stressRegenerationInnate immunityMolecules expressionOxidative stress |
| spellingShingle | Claudia La Corte Stephanie Barnay-Verdier Paola Furla Luca Bisanti Mariano Dara Gabriele Rizzuto Salvatrice Vizzini Maria Giovanna Parisi Matteo Cammarata Integrated approach to explore Anemonia viridis regeneration under a climate change scenario Scientific Reports Thermal stress Regeneration Innate immunity Molecules expression Oxidative stress |
| title | Integrated approach to explore Anemonia viridis regeneration under a climate change scenario |
| title_full | Integrated approach to explore Anemonia viridis regeneration under a climate change scenario |
| title_fullStr | Integrated approach to explore Anemonia viridis regeneration under a climate change scenario |
| title_full_unstemmed | Integrated approach to explore Anemonia viridis regeneration under a climate change scenario |
| title_short | Integrated approach to explore Anemonia viridis regeneration under a climate change scenario |
| title_sort | integrated approach to explore anemonia viridis regeneration under a climate change scenario |
| topic | Thermal stress Regeneration Innate immunity Molecules expression Oxidative stress |
| url | https://doi.org/10.1038/s41598-025-11041-7 |
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