Epidemiological monitoring of avian influenza in the Republic of Crimea in 2019–2020
The paper presents results of avian influenza epidemiological monitoring in the Republic of Crimea in 2019–2020. The attention was focused on the study of water basins of the Azov and Black Seas, the Sivash Lagoon and freshwater lakes in the Feodosia Urban Okrug, Leninsky, Sovetsky, Nizhnegorsky, Ch...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2021-11-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/592 |
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| author | D. V. Gadzevich S. I. Danylchenko N. V. Vorotilova M. A. Pasunkina V. A. Uppe V. N. Irza M. S. Volkov N. G. Zinyakov |
| author_facet | D. V. Gadzevich S. I. Danylchenko N. V. Vorotilova M. A. Pasunkina V. A. Uppe V. N. Irza M. S. Volkov N. G. Zinyakov |
| author_sort | D. V. Gadzevich |
| collection | DOAJ |
| description | The paper presents results of avian influenza epidemiological monitoring in the Republic of Crimea in 2019–2020. The attention was focused on the study of water basins of the Azov and Black Seas, the Sivash Lagoon and freshwater lakes in the Feodosia Urban Okrug, Leninsky, Sovetsky, Nizhnegorsky, Chernomorsky and Saksky Raions to detect the avian influenza virus circulation. Examination of the above mentioned areas showed that some freshwater reservoirs became shallow and dry, and aquatic vegetation degraded. The natural biotope analysis conducted in 2019 and 2020 showed a decreased number of semiaquatic wild birds. The pathological material was sampled from semiaquatic and migratory wild birds, as well as from poultry kept in poultry farms and backyards. The collected samples were tested using real-time RT-PCR. In 2019, the AIV type A (H9) genome was detected in one fecal sample taken from wild birds near Kuchuk-Adzhigol Lake in Feodosia Urban Okrug. The AIV type A (H5) genome was detected in 2020 during laboratory testing of pathological material taken from the remains of a mute swan within the shoreline of a freshwater lake near the Ermakovo settlement of the Dzhankoysky Raion. The genetic analysis was performed in the FGBI “ARRIAH” (Vladimir), and the N8 subtype neuraminidase of the influenza virus isolate was determined. The comparative genetic analysis of 258 bp nucleic acid sequences of the AIV H gene fragment showed that the identified isolate belongs to the Asian genetic lineage of highly pathogenic AIV subtype H5 (clade 2.3.4.4) associated with the epidemic spread in Asia, Europe, the Middle East and Africa in 2016–2020. |
| format | Article |
| id | doaj-art-ba7b14739db146908866fb0439e6fcf5 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2021-11-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-ba7b14739db146908866fb0439e6fcf52025-02-06T09:52:09ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592021-11-0110430831610.29326/2304-196X-2021-10-4-308-316535Epidemiological monitoring of avian influenza in the Republic of Crimea in 2019–2020D. V. Gadzevich0S. I. Danylchenko1N. V. Vorotilova2M. A. Pasunkina3V. A. Uppe4V. N. Irza5M. S. Volkov6N. G. Zinyakov7FGBI “ARRIAH” Branch in the Republic of CrimeaFGBI “ARRIAH” Branch in the Republic of CrimeaFGBI “ARRIAH” Branch in the Republic of CrimeaFGBI “ARRIAH” Branch in the Republic of CrimeaFGBI “ARRIAH” Branch in the Republic of CrimeaFGBI “Federal Centre for Animal Health”FGBI “Federal Centre for Animal Health”FGBI “Federal Centre for Animal Health”The paper presents results of avian influenza epidemiological monitoring in the Republic of Crimea in 2019–2020. The attention was focused on the study of water basins of the Azov and Black Seas, the Sivash Lagoon and freshwater lakes in the Feodosia Urban Okrug, Leninsky, Sovetsky, Nizhnegorsky, Chernomorsky and Saksky Raions to detect the avian influenza virus circulation. Examination of the above mentioned areas showed that some freshwater reservoirs became shallow and dry, and aquatic vegetation degraded. The natural biotope analysis conducted in 2019 and 2020 showed a decreased number of semiaquatic wild birds. The pathological material was sampled from semiaquatic and migratory wild birds, as well as from poultry kept in poultry farms and backyards. The collected samples were tested using real-time RT-PCR. In 2019, the AIV type A (H9) genome was detected in one fecal sample taken from wild birds near Kuchuk-Adzhigol Lake in Feodosia Urban Okrug. The AIV type A (H5) genome was detected in 2020 during laboratory testing of pathological material taken from the remains of a mute swan within the shoreline of a freshwater lake near the Ermakovo settlement of the Dzhankoysky Raion. The genetic analysis was performed in the FGBI “ARRIAH” (Vladimir), and the N8 subtype neuraminidase of the influenza virus isolate was determined. The comparative genetic analysis of 258 bp nucleic acid sequences of the AIV H gene fragment showed that the identified isolate belongs to the Asian genetic lineage of highly pathogenic AIV subtype H5 (clade 2.3.4.4) associated with the epidemic spread in Asia, Europe, the Middle East and Africa in 2016–2020.https://veterinary.arriah.ru/jour/article/view/592wild migratory birdssemiaquatic birdsavian influenzamigrationbird faunaepidemiological monitoring |
| spellingShingle | D. V. Gadzevich S. I. Danylchenko N. V. Vorotilova M. A. Pasunkina V. A. Uppe V. N. Irza M. S. Volkov N. G. Zinyakov Epidemiological monitoring of avian influenza in the Republic of Crimea in 2019–2020 Ветеринария сегодня wild migratory birds semiaquatic birds avian influenza migration bird fauna epidemiological monitoring |
| title | Epidemiological monitoring of avian influenza in the Republic of Crimea in 2019–2020 |
| title_full | Epidemiological monitoring of avian influenza in the Republic of Crimea in 2019–2020 |
| title_fullStr | Epidemiological monitoring of avian influenza in the Republic of Crimea in 2019–2020 |
| title_full_unstemmed | Epidemiological monitoring of avian influenza in the Republic of Crimea in 2019–2020 |
| title_short | Epidemiological monitoring of avian influenza in the Republic of Crimea in 2019–2020 |
| title_sort | epidemiological monitoring of avian influenza in the republic of crimea in 2019 2020 |
| topic | wild migratory birds semiaquatic birds avian influenza migration bird fauna epidemiological monitoring |
| url | https://veterinary.arriah.ru/jour/article/view/592 |
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