PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION
Background: Filariasis patients can be a source of transmission if their blood still contains microfilariae. One of the Polymerase Chain Reaction (PCR) methods used is High Resolution Melting (HRM), using primary specificity testing. Purpose: To test the specificity of SLX primer. The samples used f...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga
2021-07-01
|
| Series: | Journal of Vocational Health Studies |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JVHS/article/view/26932 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832574184431026176 |
|---|---|
| author | Bagus Muhammad Ihsan Cecep Dani Sucipto Khayan Khayan |
| author_facet | Bagus Muhammad Ihsan Cecep Dani Sucipto Khayan Khayan |
| author_sort | Bagus Muhammad Ihsan |
| collection | DOAJ |
| description | Background: Filariasis patients can be a source of transmission if their blood still contains microfilariae. One of the Polymerase Chain Reaction (PCR) methods used is High Resolution Melting (HRM), using primary specificity testing. Purpose: To test the specificity of SLX primer. The samples used for this test were isolates of Salmonella., Klebsiella, Pseudomonas, negative and positive controls for Brugia malayi and Wuchereria bancrofti. Method: The design in this study is a quasi-experiment by testing the specificity of SLX primer using HRMbased real-time PCR based on the Cycle Threshold (CT) value observed through the amplification curve. Result: The real-time PCR results showed that no CT was released in the bacterial samples, and there was a CT value in the positive control. The results of this study indicate that specific SLX primer can be used in identifying microfilariae. Conclusion: SLX primer have a reasonable specificity because they cannot detect the existence of microorganisms in the samples other than microfilariae. |
| format | Article |
| id | doaj-art-ba5a22275f8048afb77df9bc65a77030 |
| institution | Kabale University |
| issn | 2580-7161 2580-717X |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Vocational Health Studies |
| spelling | doaj-art-ba5a22275f8048afb77df9bc65a770302025-02-02T00:38:29ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2021-07-0151263010.20473/jvhs.V5.I1.2021.26-3013159PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATIONBagus Muhammad Ihsan0Cecep Dani Sucipto1Khayan Khayan2Poltekkes Kemenkes BantenPoltekkes Kemenkes BantenPoltekkes Kemenkes BantenBackground: Filariasis patients can be a source of transmission if their blood still contains microfilariae. One of the Polymerase Chain Reaction (PCR) methods used is High Resolution Melting (HRM), using primary specificity testing. Purpose: To test the specificity of SLX primer. The samples used for this test were isolates of Salmonella., Klebsiella, Pseudomonas, negative and positive controls for Brugia malayi and Wuchereria bancrofti. Method: The design in this study is a quasi-experiment by testing the specificity of SLX primer using HRMbased real-time PCR based on the Cycle Threshold (CT) value observed through the amplification curve. Result: The real-time PCR results showed that no CT was released in the bacterial samples, and there was a CT value in the positive control. The results of this study indicate that specific SLX primer can be used in identifying microfilariae. Conclusion: SLX primer have a reasonable specificity because they cannot detect the existence of microorganisms in the samples other than microfilariae.https://e-journal.unair.ac.id/JVHS/article/view/26932mikrofilariareal-time pcrspesifitasprimer slx |
| spellingShingle | Bagus Muhammad Ihsan Cecep Dani Sucipto Khayan Khayan PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION Journal of Vocational Health Studies mikrofilaria real-time pcr spesifitas primer slx |
| title | PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION |
| title_full | PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION |
| title_fullStr | PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION |
| title_full_unstemmed | PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION |
| title_short | PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION |
| title_sort | primary slx test using real time pcr based on high resolution melting hrm on microfilaria examination |
| topic | mikrofilaria real-time pcr spesifitas primer slx |
| url | https://e-journal.unair.ac.id/JVHS/article/view/26932 |
| work_keys_str_mv | AT bagusmuhammadihsan primaryslxtestusingrealtimepcrbasedonhighresolutionmeltinghrmonmicrofilariaexamination AT cecepdanisucipto primaryslxtestusingrealtimepcrbasedonhighresolutionmeltinghrmonmicrofilariaexamination AT khayankhayan primaryslxtestusingrealtimepcrbasedonhighresolutionmeltinghrmonmicrofilariaexamination |