Maternal blood proteomics during relapse of early preeclampsia
Aim: to study the contribution of maternal blood endothelial proteins to developing relapse of early preeclampsia (ePE).Materials and Methods. A proteomic analysis of the peripheral blood of 137 pregnant women was performed. Clinically, three groups were identified at the end of pregnancy: control (...
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IRBIS LLC
2024-01-01
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| Series: | Акушерство, гинекология и репродукция |
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| Online Access: | https://www.gynecology.su/jour/article/view/1843 |
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| author | M. G. Nikolaeva V. Yu. Terekhina A. P. Momot |
| author_facet | M. G. Nikolaeva V. Yu. Terekhina A. P. Momot |
| author_sort | M. G. Nikolaeva |
| collection | DOAJ |
| description | Aim: to study the contribution of maternal blood endothelial proteins to developing relapse of early preeclampsia (ePE).Materials and Methods. A proteomic analysis of the peripheral blood of 137 pregnant women was performed. Clinically, three groups were identified at the end of pregnancy: control (n = 40), patients with favorable course of the current and previous pregnancy; comparison group (n = 59) – patients with a history of еPE episode, but favorable course of ongoing pregnancy, and main group (n = 38) – patients with еPE relapse. Biologically active substances evidencing about impaired endothelial function were subject to dynamic monitoring (11–13, 19–21 and 27–28 weeks): activity of endothelin-1 (ET-1) and metalloproteinase ADAMTS-13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13), von Willebrand factor (vWF) level and homocysteine (HC) concentration. The ADAMTS-13/vWF ratio was evaluated separately.Results. For patients with recurrent еPE, a significant increase in ET-1 is characteristic at all stages of gestation: 0.92; 1.07 and 1.36 pmol/ml vs. 0.29; 0.33 and 0.29 pmol/ml in the control group (p < 0.0001 at all points). Regardless of pregnancy outcome, increasing gestational age was paralleled with elevating vWF level, however, upon еPE relapse, this parameter (Me = 343 IU) is significantly higher (p < 0.0001) than in control group (Me = 260 IU). In all groups, there was a significant decrease in ADAMTS-13 activity, whereas in main group ADAMTS-13 activity at first time point was minimal – 63.4 % (p = 0.0007 relative to control group). With regard to ADAMTS-13/vWF axis in relapsed еPE, significant differences were found compared with control group both at 11–13 weeks (0.32 vs. 0.52; p < 0.0001) and at 27–28 weeks (0.15 vs. 0.22; p < 0.0001) pregnancy. The HC concentration declines with gestational age, but at first time point patients from main group had it (Me = 8.0 µmol/L) at significantly higher level than in control group (Me = 5.9 µmol/L; p < 0.00010).Conclusion. At gestational age of 11–13 weeks, all analyzed biomarkers contribute to developing еPE relapse accounting for an overall impact of 62.3 % of developing ePE risk. During pregnancy at 19–21 weeks, an imbalance in the ADAMTS-13/vWF along with elevated ET-1 level determine the risk of disease relapse in 65.6 % of cases. It was found that at a gestational age of 27–28 weeks, the associated shift in ET-1, vWF and ADAMTS-13 magnitude accounts for 67.9 % of risk for disease relapse. |
| format | Article |
| id | doaj-art-ba2ef28207c84f93bfe1dd55fd6ab708 |
| institution | DOAJ |
| issn | 2313-7347 2500-3194 |
| language | Russian |
| publishDate | 2024-01-01 |
| publisher | IRBIS LLC |
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| series | Акушерство, гинекология и репродукция |
| spelling | doaj-art-ba2ef28207c84f93bfe1dd55fd6ab7082025-08-20T03:19:15ZrusIRBIS LLCАкушерство, гинекология и репродукция2313-73472500-31942024-01-0117671872810.17749/2313-7347/ob.gyn.rep.2023.460843Maternal blood proteomics during relapse of early preeclampsiaM. G. Nikolaeva0V. Yu. Terekhina1A. P. Momot2Altai State Medical University, Health Ministry of Russian Federation; Altay Branch of National Medical Research Center for Hematology, Health Ministry of Russian FederationAltai State Medical University, Health Ministry of Russian FederationAltai State Medical University, Health Ministry of Russian Federation; Altay Branch of National Medical Research Center for Hematology, Health Ministry of Russian FederationAim: to study the contribution of maternal blood endothelial proteins to developing relapse of early preeclampsia (ePE).Materials and Methods. A proteomic analysis of the peripheral blood of 137 pregnant women was performed. Clinically, three groups were identified at the end of pregnancy: control (n = 40), patients with favorable course of the current and previous pregnancy; comparison group (n = 59) – patients with a history of еPE episode, but favorable course of ongoing pregnancy, and main group (n = 38) – patients with еPE relapse. Biologically active substances evidencing about impaired endothelial function were subject to dynamic monitoring (11–13, 19–21 and 27–28 weeks): activity of endothelin-1 (ET-1) and metalloproteinase ADAMTS-13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13), von Willebrand factor (vWF) level and homocysteine (HC) concentration. The ADAMTS-13/vWF ratio was evaluated separately.Results. For patients with recurrent еPE, a significant increase in ET-1 is characteristic at all stages of gestation: 0.92; 1.07 and 1.36 pmol/ml vs. 0.29; 0.33 and 0.29 pmol/ml in the control group (p < 0.0001 at all points). Regardless of pregnancy outcome, increasing gestational age was paralleled with elevating vWF level, however, upon еPE relapse, this parameter (Me = 343 IU) is significantly higher (p < 0.0001) than in control group (Me = 260 IU). In all groups, there was a significant decrease in ADAMTS-13 activity, whereas in main group ADAMTS-13 activity at first time point was minimal – 63.4 % (p = 0.0007 relative to control group). With regard to ADAMTS-13/vWF axis in relapsed еPE, significant differences were found compared with control group both at 11–13 weeks (0.32 vs. 0.52; p < 0.0001) and at 27–28 weeks (0.15 vs. 0.22; p < 0.0001) pregnancy. The HC concentration declines with gestational age, but at first time point patients from main group had it (Me = 8.0 µmol/L) at significantly higher level than in control group (Me = 5.9 µmol/L; p < 0.00010).Conclusion. At gestational age of 11–13 weeks, all analyzed biomarkers contribute to developing еPE relapse accounting for an overall impact of 62.3 % of developing ePE risk. During pregnancy at 19–21 weeks, an imbalance in the ADAMTS-13/vWF along with elevated ET-1 level determine the risk of disease relapse in 65.6 % of cases. It was found that at a gestational age of 27–28 weeks, the associated shift in ET-1, vWF and ADAMTS-13 magnitude accounts for 67.9 % of risk for disease relapse.https://www.gynecology.su/jour/article/view/1843relapse of early preeclampsiaepeendothelin-1et-1von willebrand factorvwfmetalloproteinase adamts-13homocysteinehcendothelium |
| spellingShingle | M. G. Nikolaeva V. Yu. Terekhina A. P. Momot Maternal blood proteomics during relapse of early preeclampsia Акушерство, гинекология и репродукция relapse of early preeclampsia epe endothelin-1 et-1 von willebrand factor vwf metalloproteinase adamts-13 homocysteine hc endothelium |
| title | Maternal blood proteomics during relapse of early preeclampsia |
| title_full | Maternal blood proteomics during relapse of early preeclampsia |
| title_fullStr | Maternal blood proteomics during relapse of early preeclampsia |
| title_full_unstemmed | Maternal blood proteomics during relapse of early preeclampsia |
| title_short | Maternal blood proteomics during relapse of early preeclampsia |
| title_sort | maternal blood proteomics during relapse of early preeclampsia |
| topic | relapse of early preeclampsia epe endothelin-1 et-1 von willebrand factor vwf metalloproteinase adamts-13 homocysteine hc endothelium |
| url | https://www.gynecology.su/jour/article/view/1843 |
| work_keys_str_mv | AT mgnikolaeva maternalbloodproteomicsduringrelapseofearlypreeclampsia AT vyuterekhina maternalbloodproteomicsduringrelapseofearlypreeclampsia AT apmomot maternalbloodproteomicsduringrelapseofearlypreeclampsia |