Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions
Microenvironment plays an important role for stem cell proliferation and differentiation. Macromolecular crowding (MMC) was recently shown to assist stem cells in forming their own matrix microenvironment in vitro. The ability of MMC to support adipose stem cell (ASC) proliferation, metabolism, and m...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/6909163 |
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| author | Mimmi Patrikoski Michelle Hui Ching Lee Laura Mäkinen Xiu Min Ang Bettina Mannerström Michael Raghunath Susanna Miettinen |
| author_facet | Mimmi Patrikoski Michelle Hui Ching Lee Laura Mäkinen Xiu Min Ang Bettina Mannerström Michael Raghunath Susanna Miettinen |
| author_sort | Mimmi Patrikoski |
| collection | DOAJ |
| description | Microenvironment plays an important role for stem cell proliferation and differentiation. Macromolecular crowding (MMC) was recently shown to assist stem cells in forming their own matrix microenvironment in vitro. The ability of MMC to support adipose stem cell (ASC) proliferation, metabolism, and multilineage differentiation was studied under different conditions: fetal bovine serum- (FBS-) and human serum- (HS-) based media and xeno- and serum-free (XF/SF) media. Furthermore, the immunophenotype of ASCs under MMC was evaluated. The proliferative capacity of ASCs under MMC was attenuated in each condition. However, osteogenic differentiation was enhanced under MMC, shown by increased deposition of mineralized matrix in FBS and HS cultures. Likewise, significantly greater lipid droplet accumulation and increased collagen IV deposition indicated enhanced adipogenesis under MMC in FBS and HS cultures. In contrast, chondrogenic differentiation was attenuated in ASCs expanded under MMC. The ASC immunophenotype was maintained under MMC with significantly higher expression of CD54. However, MMC impaired metabolic activity and differentiation capacity of ASCs in XF/SF conditions. Both the supportive and inhibitory effects of MMC on ASC are culture condition dependent. In the presence of serum, MMC maintains ASC immunophenotype and enhances adipogenic and osteogenic differentiation at the cost of reduced proliferation. |
| format | Article |
| id | doaj-art-b9f0296910394190ab1cb46447713ca5 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b9f0296910394190ab1cb46447713ca52025-02-03T05:59:46ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/69091636909163Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free ConditionsMimmi Patrikoski0Michelle Hui Ching Lee1Laura Mäkinen2Xiu Min Ang3Bettina Mannerström4Michael Raghunath5Susanna Miettinen6Adult Stem Cell Group, BioMediTech, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, FinlandNUS Tissue Engineering Program, Life Sciences Institute, National University of Singapore, 117510, SingaporeAdult Stem Cell Group, BioMediTech, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, FinlandNUS Tissue Engineering Program, Life Sciences Institute, National University of Singapore, 117510, SingaporeAdult Stem Cell Group, BioMediTech, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, FinlandNUS Tissue Engineering Program, Life Sciences Institute, National University of Singapore, 117510, SingaporeAdult Stem Cell Group, BioMediTech, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, FinlandMicroenvironment plays an important role for stem cell proliferation and differentiation. Macromolecular crowding (MMC) was recently shown to assist stem cells in forming their own matrix microenvironment in vitro. The ability of MMC to support adipose stem cell (ASC) proliferation, metabolism, and multilineage differentiation was studied under different conditions: fetal bovine serum- (FBS-) and human serum- (HS-) based media and xeno- and serum-free (XF/SF) media. Furthermore, the immunophenotype of ASCs under MMC was evaluated. The proliferative capacity of ASCs under MMC was attenuated in each condition. However, osteogenic differentiation was enhanced under MMC, shown by increased deposition of mineralized matrix in FBS and HS cultures. Likewise, significantly greater lipid droplet accumulation and increased collagen IV deposition indicated enhanced adipogenesis under MMC in FBS and HS cultures. In contrast, chondrogenic differentiation was attenuated in ASCs expanded under MMC. The ASC immunophenotype was maintained under MMC with significantly higher expression of CD54. However, MMC impaired metabolic activity and differentiation capacity of ASCs in XF/SF conditions. Both the supportive and inhibitory effects of MMC on ASC are culture condition dependent. In the presence of serum, MMC maintains ASC immunophenotype and enhances adipogenic and osteogenic differentiation at the cost of reduced proliferation.http://dx.doi.org/10.1155/2017/6909163 |
| spellingShingle | Mimmi Patrikoski Michelle Hui Ching Lee Laura Mäkinen Xiu Min Ang Bettina Mannerström Michael Raghunath Susanna Miettinen Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions Stem Cells International |
| title | Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions |
| title_full | Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions |
| title_fullStr | Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions |
| title_full_unstemmed | Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions |
| title_short | Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum, and Defined Xeno-Free/Serum-Free Conditions |
| title_sort | effects of macromolecular crowding on human adipose stem cell culture in fetal bovine serum human serum and defined xeno free serum free conditions |
| url | http://dx.doi.org/10.1155/2017/6909163 |
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