Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae)
Enzyme immobilization is a recovery technique that has been studied in several years, using support as a media to help enzyme dissolutions to the reaction substrate. Immobilization method used in this study was adsorption method, using specific lipase from Aspergillus oryzae. Lipase was partially pu...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/608075 |
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| author | Seniwati Dali A. B. D. Rauf Patong M. Noor Jalaluddin Pirman Baharuddin Hamzah |
| author_facet | Seniwati Dali A. B. D. Rauf Patong M. Noor Jalaluddin Pirman Baharuddin Hamzah |
| author_sort | Seniwati Dali |
| collection | DOAJ |
| description | Enzyme immobilization is a recovery technique that has been studied in several years, using support as a media to help enzyme dissolutions to the reaction substrate. Immobilization method used in this study was adsorption method, using specific lipase from Aspergillus oryzae. Lipase was partially purified from the culture supernatant of Aspergillus oryzae. Enzyme was immobilized by adsorbed on silica gel. Studies on free and immobilized lipase systems for determination of optimum pH, optimum temperature, thermal stability and reusability were carried out. The results showed that free lipase had optimum pH 8,2 and optimum temperature 35 °C while the immobilized lipase had optimum 8,2 and optimum temperature 45 °C. The thermal stability of the immobilized lipase, relative to that of the free lipase, was markedly increased. The immobilized lipase can be reused for at least six times. |
| format | Article |
| id | doaj-art-b9e35093ece94caa802b58e77cc8a470 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-b9e35093ece94caa802b58e77cc8a4702025-02-03T06:11:25ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-018289690210.1155/2011/608075Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae)Seniwati Dali0A. B. D. Rauf Patong1M. Noor Jalaluddin2Pirman3Baharuddin Hamzah4Chemistry Department, Faculty of Mathematics and Natural SciencesHasanuddin University, Makassar, IndonesiaChemistry Department, Faculty of Mathematics and Natural SciencesHasanuddin University, Makassar, IndonesiaChemistry Department, Faculty of Mathematics and Natural SciencesHasanuddin University, Makassar, IndonesiaMakassar Indonesia, Chemical Engineering State of Polytechnic Ujung Pandang, Makassar IndonesiaChemistry Department, Faculty of Teacher Training and Education, IndonesiaTadulako University, Palu, IndonesiaEnzyme immobilization is a recovery technique that has been studied in several years, using support as a media to help enzyme dissolutions to the reaction substrate. Immobilization method used in this study was adsorption method, using specific lipase from Aspergillus oryzae. Lipase was partially purified from the culture supernatant of Aspergillus oryzae. Enzyme was immobilized by adsorbed on silica gel. Studies on free and immobilized lipase systems for determination of optimum pH, optimum temperature, thermal stability and reusability were carried out. The results showed that free lipase had optimum pH 8,2 and optimum temperature 35 °C while the immobilized lipase had optimum 8,2 and optimum temperature 45 °C. The thermal stability of the immobilized lipase, relative to that of the free lipase, was markedly increased. The immobilized lipase can be reused for at least six times.http://dx.doi.org/10.1155/2011/608075 |
| spellingShingle | Seniwati Dali A. B. D. Rauf Patong M. Noor Jalaluddin Pirman Baharuddin Hamzah Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae) E-Journal of Chemistry |
| title | Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae) |
| title_full | Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae) |
| title_fullStr | Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae) |
| title_full_unstemmed | Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae) |
| title_short | Immobilization of Isolated Lipase From Moldy Copra (Aspergillus Oryzae) |
| title_sort | immobilization of isolated lipase from moldy copra aspergillus oryzae |
| url | http://dx.doi.org/10.1155/2011/608075 |
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