The interaction of Adipose Derived Stem Cells and Breast Cancer
Introduction: Mesenchymal stem cells are adult stem cells capable of self-renewal and multilineage differentiation (Schweizer et al., 2015). Adipose derived stem cells have been used in breast reconstruction following surgical intervention in breast cancer patients. MicroRNAs have been linked to gen...
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| Format: | Article |
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Technological University Dublin
2022-12-01
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| Series: | SURE Journal: (Science Undergraduate Research Experience Journal) |
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| Online Access: | https://arrow.tudublin.ie/sure_j/vol4/iss1/6 |
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| author | Natasha Cunningham Ava O'Meara Cathy L. Brougham |
| author_facet | Natasha Cunningham Ava O'Meara Cathy L. Brougham |
| author_sort | Natasha Cunningham |
| collection | DOAJ |
| description | Introduction: Mesenchymal stem cells are adult stem cells capable of self-renewal and multilineage differentiation (Schweizer et al., 2015). Adipose derived stem cells have been used in breast reconstruction following surgical intervention in breast cancer patients. MicroRNAs have been linked to gene regulation essential in oncogenic, and tumour suppression as well as cell signalling pathways in BC.
Aim: To research the hypothesis of ADSCs and their therapeutic properties in BC patients.
Methods: Proliferation assays were carried out to demonstrate how ADSC conditioned media influenced BC cell lines MDA-MB-231, SKBR3, and T47D. The expression of six miRNAs (miR-21, miR-133, miR-222, miR-146, miR-221, and miR-A) and three cytokines (TGF-β, RANTES, TNF- α) was determined using a variety of functional assays. Statistical analysis was performed using Minitab 20.1.0.
Findings: Upregulation of miRNA expression all miR-21 co-culture samples, miR-222 T47D co-culture, and both miR-146, and miR-221’s SKBR3 BC co-culture cell line. All co-culture cells within miR-133 expression displayed downregulation of high significance, and co-culture cells lines MDA-MB-231 and SKBR3 expressing miR-222. Down-regulation was observed in all cytokine samples (p) of BC cell co-cultures, apart from RANTES concentration within SKBR3 (p). This research demonstrated how ADSCs have properties as a double-edged sword by providing insight upon ADSCs influence on BC malignancy properties. Which in future could be a target for novel treatment therapies, thus giving patients a better prognosis and survival rate by providing a personalised molecular approach of treatment. |
| format | Article |
| id | doaj-art-b9dd48f064404c988fb5f2723e19d952 |
| institution | Kabale University |
| issn | 2990-8167 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Technological University Dublin |
| record_format | Article |
| series | SURE Journal: (Science Undergraduate Research Experience Journal) |
| spelling | doaj-art-b9dd48f064404c988fb5f2723e19d9522025-01-31T10:28:14ZengTechnological University DublinSURE Journal: (Science Undergraduate Research Experience Journal)2990-81672022-12-014110.21427/x6hh-0w87The interaction of Adipose Derived Stem Cells and Breast CancerNatasha Cunningham0Ava O'Meara1Cathy L. Brougham2Technological University of the Shannon: MidlandsTechnological University of the Shannon: MidlandsTechnological University of the Shannon: MidlandsIntroduction: Mesenchymal stem cells are adult stem cells capable of self-renewal and multilineage differentiation (Schweizer et al., 2015). Adipose derived stem cells have been used in breast reconstruction following surgical intervention in breast cancer patients. MicroRNAs have been linked to gene regulation essential in oncogenic, and tumour suppression as well as cell signalling pathways in BC. Aim: To research the hypothesis of ADSCs and their therapeutic properties in BC patients. Methods: Proliferation assays were carried out to demonstrate how ADSC conditioned media influenced BC cell lines MDA-MB-231, SKBR3, and T47D. The expression of six miRNAs (miR-21, miR-133, miR-222, miR-146, miR-221, and miR-A) and three cytokines (TGF-β, RANTES, TNF- α) was determined using a variety of functional assays. Statistical analysis was performed using Minitab 20.1.0. Findings: Upregulation of miRNA expression all miR-21 co-culture samples, miR-222 T47D co-culture, and both miR-146, and miR-221’s SKBR3 BC co-culture cell line. All co-culture cells within miR-133 expression displayed downregulation of high significance, and co-culture cells lines MDA-MB-231 and SKBR3 expressing miR-222. Down-regulation was observed in all cytokine samples (p) of BC cell co-cultures, apart from RANTES concentration within SKBR3 (p). This research demonstrated how ADSCs have properties as a double-edged sword by providing insight upon ADSCs influence on BC malignancy properties. Which in future could be a target for novel treatment therapies, thus giving patients a better prognosis and survival rate by providing a personalised molecular approach of treatment.https://arrow.tudublin.ie/sure_j/vol4/iss1/6adscsbreast cancermicroenvironmentexosomesmirnadouble-edged swordoncological safetycytokinesproliferation |
| spellingShingle | Natasha Cunningham Ava O'Meara Cathy L. Brougham The interaction of Adipose Derived Stem Cells and Breast Cancer SURE Journal: (Science Undergraduate Research Experience Journal) adscs breast cancer microenvironment exosomes mirna double-edged sword oncological safety cytokines proliferation |
| title | The interaction of Adipose Derived Stem Cells and Breast Cancer |
| title_full | The interaction of Adipose Derived Stem Cells and Breast Cancer |
| title_fullStr | The interaction of Adipose Derived Stem Cells and Breast Cancer |
| title_full_unstemmed | The interaction of Adipose Derived Stem Cells and Breast Cancer |
| title_short | The interaction of Adipose Derived Stem Cells and Breast Cancer |
| title_sort | interaction of adipose derived stem cells and breast cancer |
| topic | adscs breast cancer microenvironment exosomes mirna double-edged sword oncological safety cytokines proliferation |
| url | https://arrow.tudublin.ie/sure_j/vol4/iss1/6 |
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