Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activity

Arctium lappa is a therapeutic plant high in bioactive components, including flavonoids, which have antioxidant, anti-inflammatory, and antibacterial effects. Quercetin, a major flavonoid, is recognized for its pharmacological properties. The purpose of this study was to extract quercetin from A. la...

Full description

Saved in:
Bibliographic Details
Main Authors: Mahmood Zaki Lafta, Rafah Razooq Hameed Al-Samarrai, Mohamed Bouaziz
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Results in Chemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2211715625000116
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832583115010211840
author Mahmood Zaki Lafta
Rafah Razooq Hameed Al-Samarrai
Mohamed Bouaziz
author_facet Mahmood Zaki Lafta
Rafah Razooq Hameed Al-Samarrai
Mohamed Bouaziz
author_sort Mahmood Zaki Lafta
collection DOAJ
description Arctium lappa is a therapeutic plant high in bioactive components, including flavonoids, which have antioxidant, anti-inflammatory, and antibacterial effects. Quercetin, a major flavonoid, is recognized for its pharmacological properties. The purpose of this study was to extract quercetin from A. lappa and use it for the green synthesis of gold (AuNPs) and silver nanoparticles (AgNPs) to evaluate their antioxidant properties. Quercetin was extracted using the Soxhlet method with petroleum ether, chloroform, and methanol. Quercetin was purified using hydrolysis and crystallization before being characterized using HPLC, UV–Vis, FTIR, and NMR methods. Laser ablation was used to synthesize AuNPs and AgNPs, with extracted quercetin acting as a reducing agent. The nanoparticles were examined with SEM, TEM, and XRD. Antioxidant activity was measured using the DPPH free radical scavenging test, and IC50 values were generated to compare to standard ascorbic acid.HPLC indicated that the maximum quantity of quercetin (98.6 %) was achieved using chloroform extraction. AgNPs had a spherical shape via a typical size of 20–40 nm, whereas AuNPs ranged from 80-160 nm. AgNPs had the highest antioxidant capacity (89.59 %), followed by AuNPs (83.32 %) and quercetin (73.22 %). The IC50 values were 100.4 µg/mL, 120.8 µg/mL, and 146.36 µg/mL, respectively.The researchers effectively extracted quercetin from A. lappa and created AuNPs and AgNPs with significant antioxidant characteristics. AgNPs demonstrated superior free radical scavenging activity, indicating their potential use in biomedical applications, notably antioxidant therapy. More research is needed to understand their medicinal potential fully.
format Article
id doaj-art-b9c7ea1da5c6477db9fff0b301e0e30b
institution Kabale University
issn 2211-7156
language English
publishDate 2025-01-01
publisher Elsevier
record_format Article
series Results in Chemistry
spelling doaj-art-b9c7ea1da5c6477db9fff0b301e0e30b2025-01-29T05:01:01ZengElsevierResults in Chemistry2211-71562025-01-0113102028Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activityMahmood Zaki Lafta0Rafah Razooq Hameed Al-Samarrai1Mohamed Bouaziz2Laboratory of Electrochemistry and Environment, National Engineering School of Sfax, University of Sfax, BP 1173, Sfax 3038 Tunisia; Salah al-Din Education Directorate - Samarra Education Department. Samarra, Salah Al-Din, IraqDepartment of applied chemistry-College of applied science, University of Samarra, Samarra, IraqLaboratory of Electrochemistry and Environment, National Engineering School of Sfax, University of Sfax, BP 1173, Sfax 3038 Tunisia; Corresponding author.Arctium lappa is a therapeutic plant high in bioactive components, including flavonoids, which have antioxidant, anti-inflammatory, and antibacterial effects. Quercetin, a major flavonoid, is recognized for its pharmacological properties. The purpose of this study was to extract quercetin from A. lappa and use it for the green synthesis of gold (AuNPs) and silver nanoparticles (AgNPs) to evaluate their antioxidant properties. Quercetin was extracted using the Soxhlet method with petroleum ether, chloroform, and methanol. Quercetin was purified using hydrolysis and crystallization before being characterized using HPLC, UV–Vis, FTIR, and NMR methods. Laser ablation was used to synthesize AuNPs and AgNPs, with extracted quercetin acting as a reducing agent. The nanoparticles were examined with SEM, TEM, and XRD. Antioxidant activity was measured using the DPPH free radical scavenging test, and IC50 values were generated to compare to standard ascorbic acid.HPLC indicated that the maximum quantity of quercetin (98.6 %) was achieved using chloroform extraction. AgNPs had a spherical shape via a typical size of 20–40 nm, whereas AuNPs ranged from 80-160 nm. AgNPs had the highest antioxidant capacity (89.59 %), followed by AuNPs (83.32 %) and quercetin (73.22 %). The IC50 values were 100.4 µg/mL, 120.8 µg/mL, and 146.36 µg/mL, respectively.The researchers effectively extracted quercetin from A. lappa and created AuNPs and AgNPs with significant antioxidant characteristics. AgNPs demonstrated superior free radical scavenging activity, indicating their potential use in biomedical applications, notably antioxidant therapy. More research is needed to understand their medicinal potential fully.http://www.sciencedirect.com/science/article/pii/S2211715625000116QuercetinAuNPsAgNPsAnti-oxidantHPLC
spellingShingle Mahmood Zaki Lafta
Rafah Razooq Hameed Al-Samarrai
Mohamed Bouaziz
Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activity
Results in Chemistry
Quercetin
AuNPs
AgNPs
Anti-oxidant
HPLC
title Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activity
title_full Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activity
title_fullStr Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activity
title_full_unstemmed Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activity
title_short Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activity
title_sort green synthesis of silver and gold nanoparticles using quercetin extracted from arctium lappa by hplc characterization and estimation of antioxidant activity
topic Quercetin
AuNPs
AgNPs
Anti-oxidant
HPLC
url http://www.sciencedirect.com/science/article/pii/S2211715625000116
work_keys_str_mv AT mahmoodzakilafta greensynthesisofsilverandgoldnanoparticlesusingquercetinextractedfromarctiumlappabyhplccharacterizationandestimationofantioxidantactivity
AT rafahrazooqhameedalsamarrai greensynthesisofsilverandgoldnanoparticlesusingquercetinextractedfromarctiumlappabyhplccharacterizationandestimationofantioxidantactivity
AT mohamedbouaziz greensynthesisofsilverandgoldnanoparticlesusingquercetinextractedfromarctiumlappabyhplccharacterizationandestimationofantioxidantactivity