Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, China
Hydrangea macrophylla (Thunb.) Ser. is one of the widely cultivated plants in home gardens and scenic areas of China. Anthracnose disease is commonly observed during the normal growth of H. macrophylla, significantly impacting its ornamental and economic values. From 2021 to 2023, an investigation o...
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Frontiers Media S.A.
2025-01-01
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| author | Juan Zhao Juan Zhao Yanli Cheng Yanli Cheng Yayong Liu Yayong Liu Xiaojing Shi Taotao Zhang Taotao Zhang Wentao Qin Wentao Qin |
| author_facet | Juan Zhao Juan Zhao Yanli Cheng Yanli Cheng Yayong Liu Yayong Liu Xiaojing Shi Taotao Zhang Taotao Zhang Wentao Qin Wentao Qin |
| author_sort | Juan Zhao |
| collection | DOAJ |
| description | Hydrangea macrophylla (Thunb.) Ser. is one of the widely cultivated plants in home gardens and scenic areas of China. Anthracnose disease is commonly observed during the normal growth of H. macrophylla, significantly impacting its ornamental and economic values. From 2021 to 2023, an investigation on H. macrophylla anthracnose was carried out in nine parks of Beijing, China, and a total of 114 Colletotrichum isolates were obtained from the diseased leaves with typical anthracnose symptoms. Based on morphological characteristics and phylogenetic analysis of six genomic loci including rDNA-ITS, ACT, TUB2, CAL, CHS-1, and GAPDH, these isolates were identified as belonging to six Colletotrichum species. Among which, C. gloeosporioides was the most abundant (65 isolates, 57.0%), followed by C. fructicola (33 isolates, 28.9%), C. aenigma (8 isolates, 7.0%), C. truncatum (4 isolates, 3.5%), C. subacidae (2 isolates, 1.8%) and C. sojae (2 isolates, 1.8%). Pathogenicity test conducted on detached leaves of H. macrophylla revealed a distinct variation in virulence among isolates from different Colletotrichum species, and wounding was either essential or conducive to successful infection. Specifically, C. gloeosporioides exhibited greater aggressiveness, resulting in larger lesions, while C. subacidae induced lesions most quickly. Fungicide sensitivity assays demonstrated that prochloraz exerted a remarkable inhibitory effect on the mycelial growth of representative isolates belonging to the three predominant Colletotrichum species. In contrast to difenoconazole and tebuconazole, the mean EC50 values for prochloraz against C. gloeosporioides, C. fructicola, and C. aenigma were 0.062, 0.033, and 0.023 μg/ml, respectively. This is the first report of C. aenigma, C. truncatum, C. subacidae and C. sojae causing H. macrophylla anthracnose worldwide including China. These findings have elucidated the Colletotrichum species associated with H. macrophylla anthracnose as well as their fungicides sensitivities in Beijing, China. This provides a scientific foundation for the accurate diagnosis and local management of H. macrophylla anthracnose. |
| format | Article |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-b9c25bcb7f8f48388b12abcdcbf0b93b2025-01-21T08:36:44ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15041351504135Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, ChinaJuan Zhao0Juan Zhao1Yanli Cheng2Yanli Cheng3Yayong Liu4Yayong Liu5Xiaojing Shi6Taotao Zhang7Taotao Zhang8Wentao Qin9Wentao Qin10Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaCollege of Life Sciences, Yangtze University, Jingzhou, Hubei, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaDepartment of Biology, Xinzhou Normal University, Xinzhou, Shanxi, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaHydrangea macrophylla (Thunb.) Ser. is one of the widely cultivated plants in home gardens and scenic areas of China. Anthracnose disease is commonly observed during the normal growth of H. macrophylla, significantly impacting its ornamental and economic values. From 2021 to 2023, an investigation on H. macrophylla anthracnose was carried out in nine parks of Beijing, China, and a total of 114 Colletotrichum isolates were obtained from the diseased leaves with typical anthracnose symptoms. Based on morphological characteristics and phylogenetic analysis of six genomic loci including rDNA-ITS, ACT, TUB2, CAL, CHS-1, and GAPDH, these isolates were identified as belonging to six Colletotrichum species. Among which, C. gloeosporioides was the most abundant (65 isolates, 57.0%), followed by C. fructicola (33 isolates, 28.9%), C. aenigma (8 isolates, 7.0%), C. truncatum (4 isolates, 3.5%), C. subacidae (2 isolates, 1.8%) and C. sojae (2 isolates, 1.8%). Pathogenicity test conducted on detached leaves of H. macrophylla revealed a distinct variation in virulence among isolates from different Colletotrichum species, and wounding was either essential or conducive to successful infection. Specifically, C. gloeosporioides exhibited greater aggressiveness, resulting in larger lesions, while C. subacidae induced lesions most quickly. Fungicide sensitivity assays demonstrated that prochloraz exerted a remarkable inhibitory effect on the mycelial growth of representative isolates belonging to the three predominant Colletotrichum species. In contrast to difenoconazole and tebuconazole, the mean EC50 values for prochloraz against C. gloeosporioides, C. fructicola, and C. aenigma were 0.062, 0.033, and 0.023 μg/ml, respectively. This is the first report of C. aenigma, C. truncatum, C. subacidae and C. sojae causing H. macrophylla anthracnose worldwide including China. These findings have elucidated the Colletotrichum species associated with H. macrophylla anthracnose as well as their fungicides sensitivities in Beijing, China. This provides a scientific foundation for the accurate diagnosis and local management of H. macrophylla anthracnose.https://www.frontiersin.org/articles/10.3389/fpls.2024.1504135/fullHydrangea macrophyllaanthracnoseColletotrichum speciesmulti-loci phylogenyfungicide sensitivity |
| spellingShingle | Juan Zhao Juan Zhao Yanli Cheng Yanli Cheng Yayong Liu Yayong Liu Xiaojing Shi Taotao Zhang Taotao Zhang Wentao Qin Wentao Qin Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, China Frontiers in Plant Science Hydrangea macrophylla anthracnose Colletotrichum species multi-loci phylogeny fungicide sensitivity |
| title | Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, China |
| title_full | Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, China |
| title_fullStr | Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, China |
| title_full_unstemmed | Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, China |
| title_short | Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, China |
| title_sort | characterization and fungicides sensitivity of colletotrichum species causing hydrangea macrophylla anthracnose in beijing china |
| topic | Hydrangea macrophylla anthracnose Colletotrichum species multi-loci phylogeny fungicide sensitivity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1504135/full |
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