Urinary Microvesicle-Bound Uromodulin: A Potential Molecular Biomarker in Diabetic Kidney Disease
This study was designed to investigate the changes of urinary microvesicle-bound uromodulin and total urinary uromodulin levels in human urine and the correlations with the severity of diabetic kidney disease (DKD). 31 healthy subjects without diabetes and 100 patients with type 2 diabetes mellitus...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/3918681 |
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| author | Neng-jun Lou Yi-hong Ni Hong-ying Jia Jing-ti Deng Lu Jiang Feng-jie Zheng Ai-li Sun |
| author_facet | Neng-jun Lou Yi-hong Ni Hong-ying Jia Jing-ti Deng Lu Jiang Feng-jie Zheng Ai-li Sun |
| author_sort | Neng-jun Lou |
| collection | DOAJ |
| description | This study was designed to investigate the changes of urinary microvesicle-bound uromodulin and total urinary uromodulin levels in human urine and the correlations with the severity of diabetic kidney disease (DKD). 31 healthy subjects without diabetes and 100 patients with type 2 diabetes mellitus (T2DM) were included in this study. The patients with T2DM were divided into three groups based on the urinary albumin/creatinine ratio (UACR): normoalbuminuria group (DM, n=46); microalbuminuria group (DN1, n=32); and macroalbuminuria group (DN2, n=22). We use a specific monoclonal antibody AD-1 to capture the urinary microvesicles. Urinary microvesicle-bound uromodulin and total urinary uromodulin levels were determined by enzyme-linked immunosorbent assay (ELISA). Our results showed that the levels of urinary microvesicle-bound uromodulin in DN1 and DN2 groups were significantly higher than those in control group and DM group (P<0.01). Multiple stepwise linear regression analysis showed that UACR was independent determinant for urinary microvesicle-bound uromodulin (P<0.05) but not for total urinary uromodulin. These findings suggest that the levels of urinary microvesicle-bound uromodulin are associated with the severity of DKD. The uromodulin in urinary microvesicles may be a specific marker of DKD and potentially may be used to predict the onset and/or monitor the progression of DKD. |
| format | Article |
| id | doaj-art-b99cdc2514ee46e5a4e95dcc5b725d8b |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-b99cdc2514ee46e5a4e95dcc5b725d8b2025-02-03T01:11:14ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/39186813918681Urinary Microvesicle-Bound Uromodulin: A Potential Molecular Biomarker in Diabetic Kidney DiseaseNeng-jun Lou0Yi-hong Ni1Hong-ying Jia2Jing-ti Deng3Lu Jiang4Feng-jie Zheng5Ai-li Sun6The Second Hospital of Shandong University, 247 Beiyuan Street, Ji’nan, Shandong 250033, ChinaThe Second Hospital of Shandong University, 247 Beiyuan Street, Ji’nan, Shandong 250033, ChinaThe Second Hospital of Shandong University, 247 Beiyuan Street, Ji’nan, Shandong 250033, ChinaDepartment of Biochemistry, School of Medicine of Shandong University, Shandong, ChinaThe Second Hospital of Shandong University, 247 Beiyuan Street, Ji’nan, Shandong 250033, ChinaThe Second Hospital of Shandong University, 247 Beiyuan Street, Ji’nan, Shandong 250033, ChinaThe Second Hospital of Shandong University, 247 Beiyuan Street, Ji’nan, Shandong 250033, ChinaThis study was designed to investigate the changes of urinary microvesicle-bound uromodulin and total urinary uromodulin levels in human urine and the correlations with the severity of diabetic kidney disease (DKD). 31 healthy subjects without diabetes and 100 patients with type 2 diabetes mellitus (T2DM) were included in this study. The patients with T2DM were divided into three groups based on the urinary albumin/creatinine ratio (UACR): normoalbuminuria group (DM, n=46); microalbuminuria group (DN1, n=32); and macroalbuminuria group (DN2, n=22). We use a specific monoclonal antibody AD-1 to capture the urinary microvesicles. Urinary microvesicle-bound uromodulin and total urinary uromodulin levels were determined by enzyme-linked immunosorbent assay (ELISA). Our results showed that the levels of urinary microvesicle-bound uromodulin in DN1 and DN2 groups were significantly higher than those in control group and DM group (P<0.01). Multiple stepwise linear regression analysis showed that UACR was independent determinant for urinary microvesicle-bound uromodulin (P<0.05) but not for total urinary uromodulin. These findings suggest that the levels of urinary microvesicle-bound uromodulin are associated with the severity of DKD. The uromodulin in urinary microvesicles may be a specific marker of DKD and potentially may be used to predict the onset and/or monitor the progression of DKD.http://dx.doi.org/10.1155/2017/3918681 |
| spellingShingle | Neng-jun Lou Yi-hong Ni Hong-ying Jia Jing-ti Deng Lu Jiang Feng-jie Zheng Ai-li Sun Urinary Microvesicle-Bound Uromodulin: A Potential Molecular Biomarker in Diabetic Kidney Disease Journal of Diabetes Research |
| title | Urinary Microvesicle-Bound Uromodulin: A Potential Molecular Biomarker in Diabetic Kidney Disease |
| title_full | Urinary Microvesicle-Bound Uromodulin: A Potential Molecular Biomarker in Diabetic Kidney Disease |
| title_fullStr | Urinary Microvesicle-Bound Uromodulin: A Potential Molecular Biomarker in Diabetic Kidney Disease |
| title_full_unstemmed | Urinary Microvesicle-Bound Uromodulin: A Potential Molecular Biomarker in Diabetic Kidney Disease |
| title_short | Urinary Microvesicle-Bound Uromodulin: A Potential Molecular Biomarker in Diabetic Kidney Disease |
| title_sort | urinary microvesicle bound uromodulin a potential molecular biomarker in diabetic kidney disease |
| url | http://dx.doi.org/10.1155/2017/3918681 |
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