Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment
This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity fo...
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Asosiasi Sains Akuakultur Indonesia
2022-08-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/36898 |
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| author | Munti Yuhana Andreas Tambun Widanarni Widanarni Usamah Afiff |
| author_facet | Munti Yuhana Andreas Tambun Widanarni Widanarni Usamah Afiff |
| author_sort | Munti Yuhana |
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This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity for prevention against co-infection with WSSV (White Spot Syndrome Virus) and Vibrio harveyi. The synbiotic was microencapsulated by the spray dryer method. Shrimps were reared in the floating net cages in the pond. Treatments included the administration of MS at different frequencies i.e, daily (A), twice a week (B), once a week (C), and without MS supplementation (consisted of negative and positive controls) with a feeding rate of 6% of shrimp biomass (5 times a day). During the challenge trial, shrimps were removed and further reared in plastic tanks, for 7 days. The shrimps (except negative control treatment) were intramuscularly injected by WSSV filtrate at the infective dosage of 10-4 copies.ml-1. Twenty four hours after WSSV injection the shrimps were immersed in the water-containing cells suspension of V. harveyi at the cell’s population dosage of 106 CFU.ml-1. Immune responses were observed for 7 days after experimental infection. The shrimps that have been treated with daily MS supplementation (A) showed better immune responses i.e., total haemocyte counts, phenoloxidase, respiratory burst, and the lower pathogenic cells abundance in the intestine compared to other treatments groups.
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| format | Article |
| id | doaj-art-b99900601de14d33911d382ec6a8dcb1 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-b99900601de14d33911d382ec6a8dcb12025-01-24T17:56:14ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002022-08-0121210.19027/jai.21.2.125-13236898Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experimentMunti Yuhana0Andreas Tambun1Widanarni Widanarni2Usamah Afiff3Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Dramaga, West Java, Indonesia 16680Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Dramaga, West Java, Indonesia 16680Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Dramaga, West Java, Indonesia 16680Department of Animal Disease and Veterinary Public Health, IPB University This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity for prevention against co-infection with WSSV (White Spot Syndrome Virus) and Vibrio harveyi. The synbiotic was microencapsulated by the spray dryer method. Shrimps were reared in the floating net cages in the pond. Treatments included the administration of MS at different frequencies i.e, daily (A), twice a week (B), once a week (C), and without MS supplementation (consisted of negative and positive controls) with a feeding rate of 6% of shrimp biomass (5 times a day). During the challenge trial, shrimps were removed and further reared in plastic tanks, for 7 days. The shrimps (except negative control treatment) were intramuscularly injected by WSSV filtrate at the infective dosage of 10-4 copies.ml-1. Twenty four hours after WSSV injection the shrimps were immersed in the water-containing cells suspension of V. harveyi at the cell’s population dosage of 106 CFU.ml-1. Immune responses were observed for 7 days after experimental infection. The shrimps that have been treated with daily MS supplementation (A) showed better immune responses i.e., total haemocyte counts, phenoloxidase, respiratory burst, and the lower pathogenic cells abundance in the intestine compared to other treatments groups. https://journal.ipb.ac.id/index.php/jai/article/view/36898 |
| spellingShingle | Munti Yuhana Andreas Tambun Widanarni Widanarni Usamah Afiff Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment Jurnal Akuakultur Indonesia |
| title | Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment |
| title_full | Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment |
| title_fullStr | Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment |
| title_full_unstemmed | Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment |
| title_short | Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment |
| title_sort | synbiotic microcapsule dietary supplementation for prevention against co infection diseases in pacific white shrimp a limited field experiment |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/36898 |
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