The Impact of Gold Nanoparticle Susceptibility on Drug Resistance Phenotypes in Uropathogenic Escherichia coli
Introduction: The widespread use of antibiotics has contributed to the dissemination of multidrug-resistant pathogens. This study aimed to assess the prevalence of Escherichia coli strains associated with urinary tract infections, characterized by diverse drug-resistance phenotypes. Additionally...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2023-09-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | https://jommid.pasteur.ac.ir/article-1-593-en.html |
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| Summary: | Introduction: The widespread use of antibiotics has contributed to the
dissemination of multidrug-resistant pathogens. This study aimed to assess
the prevalence of Escherichia coli strains associated with urinary tract
infections, characterized by diverse drug-resistance phenotypes.
Additionally, the antibacterial properties of gold nanoparticles were
examined against each phenotype to determine their effectiveness.
Methods: This cross-sectional study was conducted on 170 E. coli strains
isolated from 250 urine samples collected from symptomatic and
asymptomatic patients with urinary tract infections (UTIs) between August
2022 and July 2023. The antibiotic susceptibility profiles across various
classes of antibiotics were determined using the Kirby-Bauer method. At the
same time, the minimum inhibitory concentrations of gold nanoparticles
were assessed through the microdilution broth test. Results: Among E. coli
isolates, 91 (53.53%), 45 (26.47%), and 17 (10%) isolates were identified as
multidrug-resistant (MDR), extensively drug-resistant (XDR), and pandrugresistant
(PDR), respectively. The MIC of gold nanoparticles that inhibited
the growth of 90% of PDR isolates (MIC90=200 ppm) was two times higher
than the MIC90 against XDR isolates (MIC90=100 ppm) and four times
higher than the MIC90 against MDR isolates (MIC90=50 ppm). There was
also a significant difference between the MIC90 and the MIC of gold
nanoparticles that inhibited the growth of 50% of PDR and XDR bacteria
(P<0.05). Conclusion: The emergence of MDR, XDR, and PDR
uropathogenic E. coli isolates represents a significant societal health
concern. Considering the favorable in vitro antimicrobial potential of gold
nanoparticles against uropathogenic E. coli isolates, it is recommended to
further analyze their applicability as antibiotic alternatives by conducting in
vivo studies. |
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| ISSN: | 2345-5349 2345-5330 |