Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone (Haliotis discus hannai Ino) muscle
A water-soluble polysaccharide from abalone muscle (AMPP) was isolated. The contents of carbohydrate, protein, uronic acid, and sulfate in AMPP were 83.5 %, 0.5 %, 2.7 %, and 2.6 %, respectively. High-performance liquid chromatography analysis indicated that AMPP was homogeneous and had an average m...
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Tsinghua University Press
2023-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302200163X |
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| author | Linfan Shi Gengxin Hao Jun Chen Junling Wang Wuyin Weng |
| author_facet | Linfan Shi Gengxin Hao Jun Chen Junling Wang Wuyin Weng |
| author_sort | Linfan Shi |
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| description | A water-soluble polysaccharide from abalone muscle (AMPP) was isolated. The contents of carbohydrate, protein, uronic acid, and sulfate in AMPP were 83.5 %, 0.5 %, 2.7 %, and 2.6 %, respectively. High-performance liquid chromatography analysis indicated that AMPP was homogeneous and had an average molecular weight of approximately 3.2 kDa. The main monosaccharides of AMPP were glucose (Glc) and mannose with a molar ratio of 99.7:0.3. The structural characteristics of AMPP were elucidated through methylation analysis, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. The linkages of AMPP consisted of terminal, 1,4-linked, 1,6-linked, and 1,4,6-linked Glcp with a molar ratio of 3.1:7.2:1.0:2.5. In one repeat unit of the proposed AMPP structure, the backbone chain was composed of eight 1→4 glycosidic bonds and one 1→6 glycosidic bond, with three branch chains linked by 1→6 glycosidic bond. In addition, AMPP was found to possess potent immunostimulatory activity via rising phagocytosis of RAW264.7 cells and promoting secretion of TNF-α. |
| format | Article |
| id | doaj-art-b95b84fa549949fabd93b68f3df966c6 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b95b84fa549949fabd93b68f3df966c62025-02-02T23:24:43ZengTsinghua University PressFood Science and Human Wellness2213-45302023-03-01122495502Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone (Haliotis discus hannai Ino) muscleLinfan Shi0Gengxin Hao1Jun Chen2Junling Wang3Wuyin Weng4College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Xiamen 361021, China; Corresponding author at: College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaA water-soluble polysaccharide from abalone muscle (AMPP) was isolated. The contents of carbohydrate, protein, uronic acid, and sulfate in AMPP were 83.5 %, 0.5 %, 2.7 %, and 2.6 %, respectively. High-performance liquid chromatography analysis indicated that AMPP was homogeneous and had an average molecular weight of approximately 3.2 kDa. The main monosaccharides of AMPP were glucose (Glc) and mannose with a molar ratio of 99.7:0.3. The structural characteristics of AMPP were elucidated through methylation analysis, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. The linkages of AMPP consisted of terminal, 1,4-linked, 1,6-linked, and 1,4,6-linked Glcp with a molar ratio of 3.1:7.2:1.0:2.5. In one repeat unit of the proposed AMPP structure, the backbone chain was composed of eight 1→4 glycosidic bonds and one 1→6 glycosidic bond, with three branch chains linked by 1→6 glycosidic bond. In addition, AMPP was found to possess potent immunostimulatory activity via rising phagocytosis of RAW264.7 cells and promoting secretion of TNF-α.http://www.sciencedirect.com/science/article/pii/S221345302200163XPolysaccharideAbalone muscleStructural characterizationImmunostimulatory activityNuclear magnetic resonance spectra |
| spellingShingle | Linfan Shi Gengxin Hao Jun Chen Junling Wang Wuyin Weng Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone (Haliotis discus hannai Ino) muscle Food Science and Human Wellness Polysaccharide Abalone muscle Structural characterization Immunostimulatory activity Nuclear magnetic resonance spectra |
| title | Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone (Haliotis discus hannai Ino) muscle |
| title_full | Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone (Haliotis discus hannai Ino) muscle |
| title_fullStr | Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone (Haliotis discus hannai Ino) muscle |
| title_full_unstemmed | Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone (Haliotis discus hannai Ino) muscle |
| title_short | Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone (Haliotis discus hannai Ino) muscle |
| title_sort | structural characterization and immunostimulatory activity of a water soluble polysaccharide from abalone haliotis discus hannai ino muscle |
| topic | Polysaccharide Abalone muscle Structural characterization Immunostimulatory activity Nuclear magnetic resonance spectra |
| url | http://www.sciencedirect.com/science/article/pii/S221345302200163X |
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