Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens
Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera, bovine hemorrhagic septicemia, enzoonotic pneumonia and swine atropic rhinitis. Two filamentous hemagglutinin genes, fhaB1 and fhaB2, are the potential virulence factors. In this study, an inactiv...
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KeAi Communications Co., Ltd.
2014-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311914608443 |
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| author | Dong-chun GUO Yan SUN Ai-qin ZHANG Jia-sen LIU Yan LU Pei-xin LIU Dong-wei YUAN Qian JIANG Chang-de SI Lian-dong QU |
| author_facet | Dong-chun GUO Yan SUN Ai-qin ZHANG Jia-sen LIU Yan LU Pei-xin LIU Dong-wei YUAN Qian JIANG Chang-de SI Lian-dong QU |
| author_sort | Dong-chun GUO |
| collection | DOAJ |
| description | Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera, bovine hemorrhagic septicemia, enzoonotic pneumonia and swine atropic rhinitis. Two filamentous hemagglutinin genes, fhaB1 and fhaB2, are the potential virulence factors. In this study, an inactivation fhaB1 mutant of P. multocida in avian strain C48-102 was constructed by a kanamycin-resistance cassette. The virulence of the fhaB1 mutant and the wild type strain was assessed in chickens by intranasal and intramuscular challenge. The inactivation of fhaB1 resulted in a high degree of attenuation when the chickens were challenged intranasally and a lesser degree when challenged intramuscularly. The fhaB1 mutant and the wild type strain were investigated their sensitivity to the antibody-dependent classical complement-mediated killing pathway in 90% convalescent chicken serum. The fhaB1 mutant was serum sensitive as the viability has reduced between untreated serum and heat inactivated chicken serum (P<0.007). These results confirmed that FhaB1 played the critical roles in the bacterial pathogenesis and further studies were needed to investigate the mechanism which caused reduced virulence of the fhaB1 mutant. |
| format | Article |
| id | doaj-art-b94d8dae7e4f41e28e4fa1d5bb66b5fa |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2014-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-b94d8dae7e4f41e28e4fa1d5bb66b5fa2025-08-20T03:57:47ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192014-10-0113102268227510.1016/S2095-3119(14)60844-3Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in ChickensDong-chun GUO0Yan SUN1Ai-qin ZHANG2Jia-sen LIU3Yan LU4Pei-xin LIU5Dong-wei YUAN6Qian JIANG7Chang-de SI8Lian-dong QU9State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.China; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 159001, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.China; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 159001, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.China; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 159001, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.China; Correspondence QU Lian-dong, Tel: +86-451-51997165Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera, bovine hemorrhagic septicemia, enzoonotic pneumonia and swine atropic rhinitis. Two filamentous hemagglutinin genes, fhaB1 and fhaB2, are the potential virulence factors. In this study, an inactivation fhaB1 mutant of P. multocida in avian strain C48-102 was constructed by a kanamycin-resistance cassette. The virulence of the fhaB1 mutant and the wild type strain was assessed in chickens by intranasal and intramuscular challenge. The inactivation of fhaB1 resulted in a high degree of attenuation when the chickens were challenged intranasally and a lesser degree when challenged intramuscularly. The fhaB1 mutant and the wild type strain were investigated their sensitivity to the antibody-dependent classical complement-mediated killing pathway in 90% convalescent chicken serum. The fhaB1 mutant was serum sensitive as the viability has reduced between untreated serum and heat inactivated chicken serum (P<0.007). These results confirmed that FhaB1 played the critical roles in the bacterial pathogenesis and further studies were needed to investigate the mechanism which caused reduced virulence of the fhaB1 mutant.http://www.sciencedirect.com/science/article/pii/S2095311914608443Pasteurella multocidafilamentous hemagglutinin B1pathogenicityvirulence |
| spellingShingle | Dong-chun GUO Yan SUN Ai-qin ZHANG Jia-sen LIU Yan LU Pei-xin LIU Dong-wei YUAN Qian JIANG Chang-de SI Lian-dong QU Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens Journal of Integrative Agriculture Pasteurella multocida filamentous hemagglutinin B1 pathogenicity virulence |
| title | Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens |
| title_full | Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens |
| title_fullStr | Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens |
| title_full_unstemmed | Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens |
| title_short | Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens |
| title_sort | construction and virulence of filamentous hemagglutinin protein b1 mutant of pasteurella multocida in chickens |
| topic | Pasteurella multocida filamentous hemagglutinin B1 pathogenicity virulence |
| url | http://www.sciencedirect.com/science/article/pii/S2095311914608443 |
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