Molecular Motors in Blood–Brain Barrier Maintenance by Astrocytes

The blood–brain barrier (BBB) comprises distinct cell types, including endothelial cells, pericytes, and astrocytes, and is essential for central nervous system (CNS) homeostasis by selectively regulating molecular transport and maintaining integrity. In particular, astrocytes are essential for BBB...

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Main Authors: Ana Filipa Sobral, Inês Costa, Vanessa Teixeira, Renata Silva, Daniel José Barbosa
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Brain Sciences
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Online Access:https://www.mdpi.com/2076-3425/15/3/279
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author Ana Filipa Sobral
Inês Costa
Vanessa Teixeira
Renata Silva
Daniel José Barbosa
author_facet Ana Filipa Sobral
Inês Costa
Vanessa Teixeira
Renata Silva
Daniel José Barbosa
author_sort Ana Filipa Sobral
collection DOAJ
description The blood–brain barrier (BBB) comprises distinct cell types, including endothelial cells, pericytes, and astrocytes, and is essential for central nervous system (CNS) homeostasis by selectively regulating molecular transport and maintaining integrity. In particular, astrocytes are essential for BBB function, as they maintain BBB integrity through their end-feet, which form a physical and biochemical interface that enhances endothelial cell function and barrier selectivity. Moreover, they secrete growth factors like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-β), which regulate tight junction (TJ) proteins (e.g., claudins and occludins) crucial for limiting paracellular permeability. Molecular motors like kinesins, dynein, and myosins are essential for these astrocyte functions. By facilitating vesicular trafficking and protein transport, they are essential for various functions, including trafficking of junctional proteins to support BBB integrity, the proper mitochondria localization within astrocyte processes for efficient energy supply, the polarized distribution of aquaporin (AQP)-4 at astrocyte end-feet for regulating water homeostasis across the BBB, and the modulation of neuroinflammatory responses. Moreover, myosin motors modulate actomyosin dynamics to regulate astrocyte process outgrowth, adhesion, migration, and morphology, facilitating their functional roles. Thus, motor protein dysregulation in astrocytes can compromise BBB function and integrity, increasing the risk of neurodegeneration. This review explores the complex interplay between astrocytes and molecular motors in regulating BBB homeostasis, which represents an attractive but poorly explored area of research.
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spelling doaj-art-b9480ca00a8d4400a3e05c5ed5a2b1032025-08-20T02:42:42ZengMDPI AGBrain Sciences2076-34252025-03-0115327910.3390/brainsci15030279Molecular Motors in Blood–Brain Barrier Maintenance by AstrocytesAna Filipa Sobral0Inês Costa1Vanessa Teixeira2Renata Silva3Daniel José Barbosa4Associate Laboratory i4HB—Institute for Health and Bioeconomy, University Institute of Health Sciences—CESPU, 4585-116 Gandra, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalAssociate Laboratory i4HB—Institute for Health and Bioeconomy, University Institute of Health Sciences—CESPU, 4585-116 Gandra, PortugalThe blood–brain barrier (BBB) comprises distinct cell types, including endothelial cells, pericytes, and astrocytes, and is essential for central nervous system (CNS) homeostasis by selectively regulating molecular transport and maintaining integrity. In particular, astrocytes are essential for BBB function, as they maintain BBB integrity through their end-feet, which form a physical and biochemical interface that enhances endothelial cell function and barrier selectivity. Moreover, they secrete growth factors like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-β), which regulate tight junction (TJ) proteins (e.g., claudins and occludins) crucial for limiting paracellular permeability. Molecular motors like kinesins, dynein, and myosins are essential for these astrocyte functions. By facilitating vesicular trafficking and protein transport, they are essential for various functions, including trafficking of junctional proteins to support BBB integrity, the proper mitochondria localization within astrocyte processes for efficient energy supply, the polarized distribution of aquaporin (AQP)-4 at astrocyte end-feet for regulating water homeostasis across the BBB, and the modulation of neuroinflammatory responses. Moreover, myosin motors modulate actomyosin dynamics to regulate astrocyte process outgrowth, adhesion, migration, and morphology, facilitating their functional roles. Thus, motor protein dysregulation in astrocytes can compromise BBB function and integrity, increasing the risk of neurodegeneration. This review explores the complex interplay between astrocytes and molecular motors in regulating BBB homeostasis, which represents an attractive but poorly explored area of research.https://www.mdpi.com/2076-3425/15/3/279molecular motorskinesinsdyneinmyosinsintracellular transportjunctional components
spellingShingle Ana Filipa Sobral
Inês Costa
Vanessa Teixeira
Renata Silva
Daniel José Barbosa
Molecular Motors in Blood–Brain Barrier Maintenance by Astrocytes
Brain Sciences
molecular motors
kinesins
dynein
myosins
intracellular transport
junctional components
title Molecular Motors in Blood–Brain Barrier Maintenance by Astrocytes
title_full Molecular Motors in Blood–Brain Barrier Maintenance by Astrocytes
title_fullStr Molecular Motors in Blood–Brain Barrier Maintenance by Astrocytes
title_full_unstemmed Molecular Motors in Blood–Brain Barrier Maintenance by Astrocytes
title_short Molecular Motors in Blood–Brain Barrier Maintenance by Astrocytes
title_sort molecular motors in blood brain barrier maintenance by astrocytes
topic molecular motors
kinesins
dynein
myosins
intracellular transport
junctional components
url https://www.mdpi.com/2076-3425/15/3/279
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