Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube
Bienzymatic acetylcholinesterase (AChE) and choline oxidase (ChOx) immobilized biosensor based on a phenyl carboxylic acid-grafted multiwalled carbon nanotube (MWNT) modified glass carbon electrode (GCE) and carbon-screen printed electrode (SPE) was fabricated for acetylcholine detection in human bl...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Advances in Materials Science and Engineering |
| Online Access: | http://dx.doi.org/10.1155/2014/971942 |
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| author | So-Ra Lee Hyo-Eun Lee Yun Ok Kang Wan-Seok Hwang Seong-Ho Choi |
| author_facet | So-Ra Lee Hyo-Eun Lee Yun Ok Kang Wan-Seok Hwang Seong-Ho Choi |
| author_sort | So-Ra Lee |
| collection | DOAJ |
| description | Bienzymatic acetylcholinesterase (AChE) and choline oxidase (ChOx) immobilized biosensor based on a phenyl carboxylic acid-grafted multiwalled carbon nanotube (MWNT) modified glass carbon electrode (GCE) and carbon-screen printed electrode (SPE) was fabricated for acetylcholine detection in human blood samples. Phenyl carboxylic acid-modified MWNT supports were prepared by electrochemical polymerization of 4-carboxyphenyl diazonium salts, which were synthesized by an amine group and sodium nitrite, on the surface of the MWNT-modified GCE and SPE in 0.1 M PBS. The successful fabrication of the AChE-ChOx-immobilized biosensor was confirmed via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV). The sensing range of the biosensor based on a GCE and SPE was 1.0~10 μM and 10~100 μM, respectively. The interfering effect of 0.1 M L-ascorbic acid, 0.1 M L-cysteine, and 0.1 M uric acid to 0.1 M acetylcholine was 3.00%, 9.00%, and 3.00%, respectively. Acetylcholine in a human blood sample was detected by the AChE-ChOx-immobilized biosensor. |
| format | Article |
| id | doaj-art-b9460a2f8aa44bf48ad0a1fc85e91715 |
| institution | Kabale University |
| issn | 1687-8434 1687-8442 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | Advances in Materials Science and Engineering |
| spelling | doaj-art-b9460a2f8aa44bf48ad0a1fc85e917152025-02-03T01:11:16ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422014-01-01201410.1155/2014/971942971942Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon NanotubeSo-Ra Lee0Hyo-Eun Lee1Yun Ok Kang2Wan-Seok Hwang3Seong-Ho Choi4Department of Chemistry, Hannam University, Daejeon 305-811, Republic of KoreaDepartment of Chemistry, Hannam University, Daejeon 305-811, Republic of KoreaDepartment of Chemistry, Hannam University, Daejeon 305-811, Republic of KoreaRedoxTech, 198-24 Myeonmok-dong, Jungnang-gu, Seoul 131-823, Republic of KoreaDepartment of Chemistry, Hannam University, Daejeon 305-811, Republic of KoreaBienzymatic acetylcholinesterase (AChE) and choline oxidase (ChOx) immobilized biosensor based on a phenyl carboxylic acid-grafted multiwalled carbon nanotube (MWNT) modified glass carbon electrode (GCE) and carbon-screen printed electrode (SPE) was fabricated for acetylcholine detection in human blood samples. Phenyl carboxylic acid-modified MWNT supports were prepared by electrochemical polymerization of 4-carboxyphenyl diazonium salts, which were synthesized by an amine group and sodium nitrite, on the surface of the MWNT-modified GCE and SPE in 0.1 M PBS. The successful fabrication of the AChE-ChOx-immobilized biosensor was confirmed via scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV). The sensing range of the biosensor based on a GCE and SPE was 1.0~10 μM and 10~100 μM, respectively. The interfering effect of 0.1 M L-ascorbic acid, 0.1 M L-cysteine, and 0.1 M uric acid to 0.1 M acetylcholine was 3.00%, 9.00%, and 3.00%, respectively. Acetylcholine in a human blood sample was detected by the AChE-ChOx-immobilized biosensor.http://dx.doi.org/10.1155/2014/971942 |
| spellingShingle | So-Ra Lee Hyo-Eun Lee Yun Ok Kang Wan-Seok Hwang Seong-Ho Choi Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube Advances in Materials Science and Engineering |
| title | Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube |
| title_full | Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube |
| title_fullStr | Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube |
| title_full_unstemmed | Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube |
| title_short | Bienzymatic Acetylcholinesterase and Choline Oxidase Immobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube |
| title_sort | bienzymatic acetylcholinesterase and choline oxidase immobilized biosensor based on a phenyl carboxylic acid grafted multiwalled carbon nanotube |
| url | http://dx.doi.org/10.1155/2014/971942 |
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