Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro
Schwann cells have been found to promote osteogenesis by an unclear molecular mechanism. To better understand how Schwann cells accelerate osteogenesis, RNA-Seq and LC-MS/MS were utilized to explore the transcriptomic and metabolic response of MC3T3-E1 to Schwann cells. Osteogenic differentiation wa...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/4363632 |
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| _version_ | 1832550928925851648 |
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| author | Jun-Qin Li Hui-Jie Jiang Xiu-Yun Su Li Feng Na-Zhi Zhan Shan-Shan Li Zi-Jie Chen Bo-Han Chang Peng-Zhen Cheng Liu Yang Guo-Xian Pei |
| author_facet | Jun-Qin Li Hui-Jie Jiang Xiu-Yun Su Li Feng Na-Zhi Zhan Shan-Shan Li Zi-Jie Chen Bo-Han Chang Peng-Zhen Cheng Liu Yang Guo-Xian Pei |
| author_sort | Jun-Qin Li |
| collection | DOAJ |
| description | Schwann cells have been found to promote osteogenesis by an unclear molecular mechanism. To better understand how Schwann cells accelerate osteogenesis, RNA-Seq and LC-MS/MS were utilized to explore the transcriptomic and metabolic response of MC3T3-E1 to Schwann cells. Osteogenic differentiation was determined by ALP staining. Lentiviruses were constructed to alter the expression of Mif (macrophage migration inhibitory factor) in Schwann cells. Western blot (WB) analysis was employed to detect the protein expression. The results of this study show that Mif is essential for Schwann cells to promote osteogenesis, and its downstream CD74/FOXO1 is also involved in the promotion of Schwann cells on osteogenesis. Further, Schwann cells regulate amino acid metabolism and lipid metabolism in preosteoblasts. These findings unveil the mechanism for Schwann cells to promote osteogenesis where Mif is a key factor. |
| format | Article |
| id | doaj-art-b9443ebd80854e96b5cc5e1475b9073e |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b9443ebd80854e96b5cc5e1475b9073e2025-02-03T06:05:31ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/4363632Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In VitroJun-Qin Li0Hui-Jie Jiang1Xiu-Yun Su2Li Feng3Na-Zhi Zhan4Shan-Shan Li5Zi-Jie Chen6Bo-Han Chang7Peng-Zhen Cheng8Liu Yang9Guo-Xian Pei10Department of OrthopaedicsDepartment of OrthopaedicsSouthern University of Science and Technology HospitalSchool of MedicineSchool of MedicineSchool of MedicineSchool of MedicineSchool of MedicineDepartment of OrthopaedicsDepartment of OrthopaedicsDepartment of OrthopaedicsSchwann cells have been found to promote osteogenesis by an unclear molecular mechanism. To better understand how Schwann cells accelerate osteogenesis, RNA-Seq and LC-MS/MS were utilized to explore the transcriptomic and metabolic response of MC3T3-E1 to Schwann cells. Osteogenic differentiation was determined by ALP staining. Lentiviruses were constructed to alter the expression of Mif (macrophage migration inhibitory factor) in Schwann cells. Western blot (WB) analysis was employed to detect the protein expression. The results of this study show that Mif is essential for Schwann cells to promote osteogenesis, and its downstream CD74/FOXO1 is also involved in the promotion of Schwann cells on osteogenesis. Further, Schwann cells regulate amino acid metabolism and lipid metabolism in preosteoblasts. These findings unveil the mechanism for Schwann cells to promote osteogenesis where Mif is a key factor.http://dx.doi.org/10.1155/2022/4363632 |
| spellingShingle | Jun-Qin Li Hui-Jie Jiang Xiu-Yun Su Li Feng Na-Zhi Zhan Shan-Shan Li Zi-Jie Chen Bo-Han Chang Peng-Zhen Cheng Liu Yang Guo-Xian Pei Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro Stem Cells International |
| title | Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro |
| title_full | Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro |
| title_fullStr | Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro |
| title_full_unstemmed | Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro |
| title_short | Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro |
| title_sort | schwann cells accelerate osteogenesis via the mif cd74 foxo1 signaling pathway in vitro |
| url | http://dx.doi.org/10.1155/2022/4363632 |
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