Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues
1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3), the active metabolite of vitamin D (Vit D), increases intestinal absorption of calcium and phosphate, maintaining a correct balance of bone remodeling. Vit D has an anabolic effect on the skeletal system and is key in promoting osteoblastic differentiation of...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/9150819 |
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| author | Francesca Posa Adriana Di Benedetto Graziana Colaianni Elisabetta A. Cavalcanti-Adam Giacomina Brunetti Chiara Porro Teresa Trotta Maria Grano Giorgio Mori |
| author_facet | Francesca Posa Adriana Di Benedetto Graziana Colaianni Elisabetta A. Cavalcanti-Adam Giacomina Brunetti Chiara Porro Teresa Trotta Maria Grano Giorgio Mori |
| author_sort | Francesca Posa |
| collection | DOAJ |
| description | 1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3), the active metabolite of vitamin D (Vit D), increases intestinal absorption of calcium and phosphate, maintaining a correct balance of bone remodeling. Vit D has an anabolic effect on the skeletal system and is key in promoting osteoblastic differentiation of human Mesenchymal Stem Cells (hMSCs) from bone marrow. MSCs can be also isolated from the immature form of the tooth, the dental bud: Dental Bud Stem Cells (DBSCs) are adult stem cells that can effectively undergo osteoblastic differentiation. In this work we investigated the effect of Vit D on DBSCs differentiation into osteoblasts. Our data demonstrate that DBSCs, cultured in an opportune osteogenic medium, differentiate into osteoblast-like cells; Vit D treatment stimulates their osteoblastic features, increasing the expression of typical markers of osteoblastogenesis like RUNX2 and Collagen I (Coll I) and, in a more important way, determining a higher production of mineralized matrix nodules. |
| format | Article |
| id | doaj-art-b93b79670a6445a2bc030964ab5377d4 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b93b79670a6445a2bc030964ab5377d42025-02-03T07:24:12ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/91508199150819Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental TissuesFrancesca Posa0Adriana Di Benedetto1Graziana Colaianni2Elisabetta A. Cavalcanti-Adam3Giacomina Brunetti4Chiara Porro5Teresa Trotta6Maria Grano7Giorgio Mori8Department of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, ItalyDepartment of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, ItalySection of Human Anatomy and Histology, Department of Basic and Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, ItalyInstitute of Physical Chemistry, Department of Biophysical Chemistry, University of Heidelberg, Heidelberg, GermanySection of Human Anatomy and Histology, Department of Basic and Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, ItalyDepartment of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, ItalyDepartment of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, ItalySection of Human Anatomy and Histology, Department of Basic and Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, ItalyDepartment of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, Italy1α,25-Dihydroxyvitamin D3 (1,25(OH)2D3), the active metabolite of vitamin D (Vit D), increases intestinal absorption of calcium and phosphate, maintaining a correct balance of bone remodeling. Vit D has an anabolic effect on the skeletal system and is key in promoting osteoblastic differentiation of human Mesenchymal Stem Cells (hMSCs) from bone marrow. MSCs can be also isolated from the immature form of the tooth, the dental bud: Dental Bud Stem Cells (DBSCs) are adult stem cells that can effectively undergo osteoblastic differentiation. In this work we investigated the effect of Vit D on DBSCs differentiation into osteoblasts. Our data demonstrate that DBSCs, cultured in an opportune osteogenic medium, differentiate into osteoblast-like cells; Vit D treatment stimulates their osteoblastic features, increasing the expression of typical markers of osteoblastogenesis like RUNX2 and Collagen I (Coll I) and, in a more important way, determining a higher production of mineralized matrix nodules.http://dx.doi.org/10.1155/2016/9150819 |
| spellingShingle | Francesca Posa Adriana Di Benedetto Graziana Colaianni Elisabetta A. Cavalcanti-Adam Giacomina Brunetti Chiara Porro Teresa Trotta Maria Grano Giorgio Mori Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues Stem Cells International |
| title | Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues |
| title_full | Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues |
| title_fullStr | Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues |
| title_full_unstemmed | Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues |
| title_short | Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues |
| title_sort | vitamin d effects on osteoblastic differentiation of mesenchymal stem cells from dental tissues |
| url | http://dx.doi.org/10.1155/2016/9150819 |
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